Church: Improving Cas9 Nature Biotech ref: http://t.co/i8H6umYzt8 Now showing 3 to 83kb deletions #ncistg14
9:10am March 21st 2014 via Hootsuite
Church:CRISPR was in '87 considered junk DNA. As of 2013: 20 organisms modified #ncistg14
9:08am March 21st 2014 via Hootsuite
Church: Slide with many ways of genome targeting - 8 methods illustrated. Meganuclease, Recombinase, lamba bet/exo MAGE, RecA CAGE #ncistg14
9:07am March 21st 2014 via Hootsuite
Church: Making double-null CCR5 with ZFN - ex-vivo deletion for HIV resistance #ncistg14
9:06am March 21st 2014 via Hootsuite
Church: Gene therapy now has been revived - ~1970 clinical trials going on. Using ZnF nuclease (ZFN, Sangamo) using FokI dimers #ncistg14
Church: 3D imaging using OligoPaints method, like x-ray crystallography 2012 PNAS ref: http://t.co/7BTThiNwpE #ncistg14
9:05am March 21st 2014 via Hootsuite
Church: Technique enables resolution to go from 400nm to 10nm #ncistg14
9:03am March 21st 2014 via Hootsuite
Church: 10nm resolution uses small labeled nt with barcoded tags to get more color space. Ref here: http://t.co/m2CeSDNoxq #ncistg14
Church: Stratification uses a subset of RNAs - not a random primer (select out certain subset of RNAs) 1/4 or 1/16th the pop. #ncistg14
9:01am March 21st 2014 via Hootsuite
Church: Resolving crowded RNA sequences - thin sections, deconvolution, super-res (10nm), molecular stratification #ncistg14
Church: Applied to iPS cells, brain tissues, fibroblast, embryos #ncistg14
9:00am March 21st 2014 via Hootsuite
Church: Depth of fibronectin example is 0 to 15 reads along the exons; 8.9kb and 527 total reads #ncistg14
Church: Not looking at 3' end, it is full-length; showed data from fibronectin mRNA incl. alt splicing and alleles #ncistg14
8:59am March 21st 2014 via Hootsuite
Church: <50% from rRNA, per-base error 0.07% shows good reprod. via r-value vs # of observations #ncistg14
8:58am March 21st 2014 via Hootsuite
Church: Entire section is now a hydrogel; rigid enough to hyb over an over, 10nm resolution. Does SOLiD sequencing, by ligation #ncistg14
8:56am March 21st 2014 via Hootsuite
Church: FISSEQ - just came out in Science (here: http://t.co/BV5W1gGp0I ) 3D RNA in-situ sequencing. Diagrammed out method #ncistg14
8:55am March 21st 2014 via Hootsuite
Church: Done in 10 cells in 384 wells; the right number to use. What about 1 cell? <1 cell? #ncistg14
8:54am March 21st 2014 via Hootsuite
Church: Real accuracy: "clinical diplotype accuracy", pointing to the CGI/Harvard paper Nature July 2012 '1 error per 10M bp' #ncistg14
Church: Oops, that should be Jay Shendure Science 2005 #ncistg14
8:52am March 21st 2014 via Hootsuite
Church: Photo of Rob Mitra, Jay Shurdure, Drmanac and their seminal contributions to NGS #ncistg14
Church: Chart with exponential quality improvement - 10^9 (Q90 quality). Haplotype phase length - on the order of 3E06 #ncistg14
8:51am March 21st 2014 via Hootsuite
Church: HGP costs and Moore's law - Affordable genome would have been 60y from 2004. Turned out to be 6y. #ncistg14
8:50am March 21st 2014 via Hootsuite
Church: "What's next for sequencing?" single-cell and sub-cellular genetics. #ncistg14
8:49am March 21st 2014 via Hootsuite
Church: Cohort with NIST+FDA http://t.co/xbCiP2kWEe project (8 trios), along with ENCODE & GTEx perfectly consented etc. #ncistg14
Church: Personal Genomes project - http://t.co/1OI2rypeha is the only open access #WGS dataset + stem cell biobank #ncistg14
8:47am March 21st 2014 via Hootsuite
Church: Pivot to CRISPR organ-on-a-chip at Wyss. Referred to Ingber's work http://t.co/JBQPe3zmtn #ncistg14
8:46am March 21st 2014 via Hootsuite
Church: Rare alleles myostatin double null example. N=1, MSTN, can do causality analysis in bovine, canine, low atheroclerosis #ncistg14
8:44am March 21st 2014 via Hootsuite
Church: Supercentenarian study - listed 9 rare protected gene variants for low coronary, low Alzhemiers, low T2D, many double-null #ncistg14
8:43am March 21st 2014 via Hootsuite
Church: Began by referencing mutations that confer extreme radiation resistance in eLife http://t.co/LL9nfH11eC #ncistg14
8:42am March 21st 2014 via Hootsuite
George Church (Harvard) at the #NCI Translational Genomics #ncistg14 meeting http://t.co/1z2XfAAzGT
8:40am March 21st 2014 via Hootsuite
George Church, Harvard: New Technologies for Disease Prevention #ncistg14
8:31am March 21st 2014 via Hootsuite
Today's agenda at the #NCI 3rd Symposia on Translational Genomics #ncistg14 http://t.co/Da2qaZUWdG Church, Staudt, Baylin
8:16am March 21st 2014 via Hootsuite
Gene silencing instructions acquired through 'molecular memory' tags on chromatin | Indiana University http://t.co/QBXRfa4E4M
7:25am March 21st 2014 via Hootsuite
RT @RWW: How I Moved Away From The Mac After Leaving Apple http://t.co/Toddg6hR8a @ocracokewaves
6:25am March 21st 2014 via Hootsuite
RT @SPACEdotcom: .@NASA’s Hubble has captured an amazing view of a distant starbirth factory. http://t.co/gdgyHjfyiU http://t.co/8Fw346g0gZ
5:25am March 21st 2014 via Hootsuite in reply to
Loblolly pine's immense genome conquered - Eureka http://t.co/x3bJAx8o2S
3:25am March 21st 2014 via Hootsuite
RT @genome_gov: New tool pinpoints genetic sources of disease, describes “overlay” of genetic and epigenetic maps http://t.co/59seDnQlCa
2:25am March 21st 2014 via Hootsuite
RT @verge: Humans can distinguish more than 1 trillion odors http://t.co/1EZF7BhNbR
1:25am March 21st 2014 via Hootsuite
RT @MicroWavesSci: In a great comment to @BioMickWatson’s post, @UseqMiseq details her computational biology journey. http://t.co/5Znylo4pZu
12:25am March 21st 2014 via Hootsuite in reply to
No triflers indeed. RT @mental_floss: 16 Ways to Find Love in the Personal Ads (in 1900) — http://t.co/88s255PDXr
11:25pm March 20th 2014 via Hootsuite
RT @ASCOPost: Immune Modulation May Aid Some Breast Cancer Subtypes http://t.co/28mf2xsySL #bcsm
10:25pm March 20th 2014 via Hootsuite
RT @DiscoverMag: The word coffee comes from an Arab word meaning “that which prevents sleep.” http://t.co/jNFbwFlJ9C
9:25pm March 20th 2014 via Hootsuite
“Chicken from hell” - nice name RT @PLOSONE: Anzu wyliei: Scientists announce new dinosaur discovery http://t.co/SpHjmwmthP via @bbcnews
8:25pm March 20th 2014 via Hootsuite in reply to
Some unexpected ones too RT @HarvardBiz: The Daily Routines of Geniuses http://t.co/QY0RvBulTA
7:25pm March 20th 2014 via Hootsuite in reply to
RT @AcademyofGrief: Grief and healing: Mom adds sandbox to baby’s grave so son can ‘play’ with brother - Today http://t.co/AIqVf2JkOi
6:25pm March 20th 2014 via Hootsuite in reply to
.@thecancergeek Here are a few of Edison Liu's publications - using ChIA-PET NGS http://t.co/tmij5mB4WI http://t.co/T5vrGmrkq1
5:38pm March 20th 2014 via Hootsuite in reply to
.@thecancergeek Yes - sorry I didn't catch Ed's reference when it was on the screen. (Alas my Windows XP machine is dying!) I'll look for it
4:13pm March 20th 2014 via Hootsuite in reply to
Farnham: CRISPRs "are so much quicker and easier to clone" (Available from @LIFECorporation http://t.co/s1CUsefq6C )#ncistg14
4:11pm March 20th 2014 via Twitter Web Client
Farnham: Now look at stable repression - of 3K promoters, 30 bound promoters stay repressed, one of which was SOX2 #ncistg14
4:10pm March 20th 2014 via Twitter Web Client
Farnham: Showed by turning on the switch, ~3K promoters methylated. But H3K4me3 unaffected (!) #ncistg14
4:08pm March 20th 2014 via Twitter Web Client
