Fleming: Mechanism illustrated from this review: http://t.co/BlLxQWYw9u Showed in-prep data (I won't tweet these) #ASM2014
2:57pm May 18th 2014 via Hootsuite
Fleming: Est 1K cells produce 2-3m of sheath/day. Able to get single-cell genome. With JGI got 40% of the genome #ASM2014
2:54pm May 18th 2014 via Hootsuite
Fleming: Described in1888 by Winogradsky. Lithotrophy and autotrophy going on in parallel. #ASM2014
2:53pm May 18th 2014 via Hootsuite
Fleming: Leptothrix ochracea sheathed filamentous FeOB, less than10% of the sheaths are occupied. Uncultured http://t.co/MVwHxBJsmk #ASM2014
2:51pm May 18th 2014 via Hootsuite
Fleming: Iron oxidizing bacteria (FeOB) - found everywhere. Biocorrosion, ditches & streams, biofouling. Iron mats form #ASM2014
2:49pm May 18th 2014 via Hootsuite
Next: Emily J. Fleming (Bigelow Laboratory of Ocean Sciences, ME) “Resolving Winogradsky's Rusty Puzzle” #ASM2014
2:47pm May 18th 2014 via Hootsuite
Popcat: Diagram from Bird et al 2011 Trends Microbiol http://t.co/mrG1ysyj9R Possibility of different e-acceptors #ASM2014
2:43pm May 18th 2014 via Hootsuite
Popcat: Extracellular changes 'in a matter of minutes'. Lower current but higher voltage by the cell; optimized for transport #ASM2014
2:41pm May 18th 2014 via Hootsuite
Popcat: Showed figure of proposed pathway from this 2008 Lovley review: http://t.co/OReuWSlyDC Shows data on possibly alt. pathways #ASM2014
2:40pm May 18th 2014 via Hootsuite
Popcat: They grow bugs at one potential, then change the redox potential. Geobacter sulfurreducens: long-range extracel. e-transfer #ASM2014
2:37pm May 18th 2014 via Hootsuite
Popcat: Lab bench: wires and bottles. They use electrochemical real-time measurement of respiration rate in mSec #ASM2014
2:34pm May 18th 2014 via Hootsuite
Sudeep Popcat, Arizona State. "Unraveling... Energy Metabolism In Members of Geobacteraceae During Extracellular Respiration" #ASM2014
2:32pm May 18th 2014 via Hootsuite
Nealson: Connected to corrosion, bioremediation, bioenergy, electrosynthesis, syntrophy... "It's all about the metals" #ASM2014
2:31pm May 18th 2014 via Hootsuite
Nealson: How electrons flow back? Controversial: nanowires, reduced electron acceptors, electrodes. A major problem to solve. #ASM2014
2:30pm May 18th 2014 via Hootsuite
Nealson:SPUDs: Self-Powered Useful Devices - not MFCs. It's not about energy, it's about water, waste removal. #ASM2014
2:29pm May 18th 2014 via Hootsuite
Nealson: Other metal reducers don't have multi-heme cytochromes, also are gram+ with no outer membrane wall. Hard to fund new study #ASM2014
2:28pm May 18th 2014 via Hootsuite
Nealson: Left anaerobic metabolism, right aerobic: 'bacterial schizo'. Similar to a biofilm if moved to its side. #ASM2014
2:27pm May 18th 2014 via Hootsuite
Nealson: A microbial fuel cell: electrons flow to cathode, protons arrive via diffusion. Same in a biofilm. #ASM2014
2:26pm May 18th 2014 via Hootsuite
Nealson: One of several pubs listed by S. Ishii, this one in ISME http://t.co/JeG49uFjCS Many bugs are not known electrogens #ASM2014
2:24pm May 18th 2014 via Hootsuite
Nealson: Change the surface potential of a 5-spp community: 3 spp change 500 genes in 100mV difference. Change back? Genes off. #ASM2014
2:21pm May 18th 2014 via Hootsuite
Nealson: Movie of a biofilm forming: Shewanella prefer the 400mV charged electrodes. What about other microbial ecology? #ASM2014
2:19pm May 18th 2014 via Hootsuite
Nealson: Use an electode - not about metal metabolism but electron flow Kim KIST #ASM2014
2:17pm May 18th 2014 via Hootsuite
Nealson: The corresponding Lovley paper for Geobacter and Fe reduction in 1988: http://t.co/FuD9HNJ9hI #ASM2014
2:15pm May 18th 2014 via Hootsuite
Nealson: 10^6-10^7 electrons / s / cell. A few fAmps/cell/s. #ASM2014
2:14pm May 18th 2014 via Hootsuite
Nealson: 10-12 hemes: to get electrons from the inside of the cell to the outside. Solves an impossible problem 10K complexes/cell #ASM2014
Nealson: Time-lapse movie in 18h a process that normally takes 10,000 years. Having cytochromes on the outside of the cell #ASM2014
2:13pm May 18th 2014 via Hootsuite
Nealson: 1988 paper in Science published at the same time as the Geobacter one http://t.co/2giMrud5Be #ASM2014
2:12pm May 18th 2014 via Hootsuite
Nealson: If it's a solid, or forms a solid, the bug will use EET to reduce the metal outside the cell #ASM2014
2:11pm May 18th 2014 via Hootsuite
Nealson: Organism was Shewanella oneidensis MR-1; corresponding Fe organism is Geobacter #ASM2014
2:10pm May 18th 2014 via Hootsuite
Nealson: Metal-rich lake sediments, from insol black to soluble MnCl2 completely clear. Repeat for pure culture #ASM2014
2:09pm May 18th 2014 via Hootsuite
Nealson: Mn reduction accounted for 50-75% of C turnover (!) in Oneida Lake #ASM2014
2:08pm May 18th 2014 via Hootsuite
Nealson: Geobiology: explaining a geochemical enigma. Oneida Lake NY had 1000x too fast electron flux. Solid MnO2 to Mn(II) #ASM2014
Nealson: Late 80's: bacteria donates electrons to solid substrates, called Extracellular Electron Transport. Thought impossible #ASM2014
2:06pm May 18th 2014 via Hootsuite
Nealson: Fuel of life: Pe(W) - electron potential in water, diagrams of donors / acceptors. methanogens at top, aerobes at bottom #ASM2014
2:04pm May 18th 2014 via Hootsuite
Nealson: Study of metals is study of electrons; virtually all electrons are carried by metals of some kind in the cell #ASM2014
2:03pm May 18th 2014 via Hootsuite
Nealson: Why electrons? "Electron flow is one of the basic features of life". Not soluble - they must be carried (conducted). con't #ASM2014
First up: Ken Nealson (USC) “Bacterial Electricians: Moving Electrons in and out of Cells” #ASM2014
2:01pm May 18th 2014 via Hootsuite
#ASM2014 Symposium: “Metals and Microbes” session organized by Ken Nealson (Univ So Calif) and Jennifer Glass (Georgia Tech)
2:00pm May 18th 2014 via Hootsuite
Interesting conversation with @T2bio - system for sepsis detection using MRI and oligo-derivatized mag beads http://t.co/qLX0xM24Ee #ASM2014
1:57pm May 18th 2014 via Hootsuite
At #ASM2014 #ASM2014 a grad student described his experiments as 'the samples were mixed with my tears'. I certainly know the feeling.
1:48pm May 18th 2014 via Hootsuite
#ASMLive Perhaps metatranscriptomics? Feel that it is more the community population rather than which genes are active. #ASM2014
11:28am May 18th 2014 via Hootsuite
#ASMLive Saliva sampling: simple expectoration into a tube, 55uL volume. Currently uses same regions as for the HMP #ASM2014
11:27am May 18th 2014 via Hootsuite
#ASMLive Demographic of their samples? Ave age 60y.o. Periodontal data? Data not available Samples are saliva #ASM2014
11:23am May 18th 2014 via Hootsuite
.@pathogenomenick From what the Loyola group said it was clear they use expanded culturing and show live bacteria. #ASM2014 #ASMLive
11:22am May 18th 2014 via Hootsuite in reply to
#ASMLive Connection to periodontal disease? They are checking saliva; shift is dramatic for panc ca; periodontal disease is complex #ASM2014
11:20am May 18th 2014 via Hootsuite
#ASMLive How to envision a molecular test? Could be as simple as a PCR-based test for a particular bacterial sp. #ASM2014
11:18am May 18th 2014 via Hootsuite
#ASMLive Human vars effect on oral microbiome e.g. Crohn's? No genotypes for their data, but signal across all affected individ's #ASM2014
11:15am May 18th 2014 via Hootsuite
#ASMLive Likely Leptotrichia shift more an effect than a cause; for colorectal cancer more a direct connection. #ASM2014
11:13am May 18th 2014 via Hootsuite
#ASMLive Leptotrichia spp. and its association - how clear the 16S data? 97% homology, they are confident of its association #ASM2014
11:12am May 18th 2014 via Hootsuite
#ASMLive Following both pancreatitis and pancreatic cancer - comparing oral microbiome over 3y. Too early to report #ASM2014
11:09am May 18th 2014 via Hootsuite
