Santangelo: Some portions are nucleosome free: on the ribosomal operon, may be due to pace of movement; 40min repl. rate #ASM2014
9:19am May 18th 2014 via Hootsuite
Santangelo: Was able to recapitulate pattern in-vitro, has histones and get same patterns. Showed promoters histone-free #ASM2014
9:18am May 18th 2014 via Hootsuite
Santangelo: T. Kodakarensis digestion of chromatin, ~60bp fragments 2013 publication: http://t.co/Y68iGpcxs0 #ASM2014
9:16am May 18th 2014 via Hootsuite
Santangelo: Archaeal transcription machinery: nucleosomes affect it; working on 3D structure #ASM2014
9:15am May 18th 2014 via Hootsuite
.@ilyachorny I reported the ~6 spammers, my feed now cleaned up. #ASM2014
9:13am May 18th 2014 via Hootsuite in reply to
Santangelo: Movie of clamp and jaw - move in synchrony. Rotation out of the way, universal movement (conserved hinge region) #ASM2014
9:11am May 18th 2014 via Hootsuite
Santangelo: Generated 75 mutants; now looking at motions of RNAP: jaw/lobe, swinging clamp, swinging stalk #ASM2014
9:10am May 18th 2014 via Hootsuite
Santangelo: Comparing Eury, Cren, Euk. Pol.I and Pol.II: uniqueness of T. kodakarensis, the simplest of the 4 #ASM2014
9:09am May 18th 2014 via Hootsuite
Santangelo: Thermococcus kodakarensis RNAP: able to get crystal structure, distinct open and closed states #ASM2014
9:08am May 18th 2014 via Hootsuite
Santangelo: Showing figure from 2009 Review Hirata Murakami http://t.co/tEGxP9AkUD about RNA polymerase and their relatedness #ASM2014
9:06am May 18th 2014 via Hootsuite
Next: Thomas Santangelo (Colorado State) "Regulated Archaeal Transcription" #ASM2014
9:00am May 18th 2014 via Hootsuite
Schleper: Finished w/ a figure from her review PubMed http://t.co/qXtCfSIYRu #ASM2014
8:59am May 18th 2014 via Hootsuite
Schleper: Bovine methanogen paper Poulsen 2013 Nature Comm PubMed: http://t.co/GNqGOwBbdD #ASM2014
8:58am May 18th 2014 via Hootsuite
Schleper: Perhaps Archaebiotics could reduce trimethylamine in vivo? #ASM2014
8:56am May 18th 2014 via Hootsuite
Schleper: Cows with rapeseed oil, transcriptomics made it clear the RCC cluster reduced methane#ASM2014
8:53am May 18th 2014 via Hootsuite
Schleper: Looking at mitigation in rumenants: methanobaceriales, also Thermoplamata looked at more closely #ASM2014
8:52am May 18th 2014 via Hootsuite
Schleper: 7th order of methanogens - started in bovine rumen. 17% of global methane output #ASM2014
Schleper: On the skin: 2013 PLOS paper http://t.co/wsTIJgup2X how could these have been overlooked in the HMP? #ASM2014
8:51am May 18th 2014 via Hootsuite
Schleper: Acidic peat evidence of same mechanism at work in recent Nature Geosci paper http://t.co/7O53NLAdKe #ASM2014
8:50am May 18th 2014 via Hootsuite
Schleper: De-convoluting the mechanism: NO2- and NH4+ contribute equally to N2O production under hi/lo O2; hybrid formaton likely #ASM2014
8:48am May 18th 2014 via Hootsuite
Schleper: Archaea also produce N2O, shown from soil microorg's, different org's produce under different O2 conditions (hi/low) #ASM2014
8:47am May 18th 2014 via Hootsuite
Schleper: Human impact on the N-cycle: chemical fixation by the Haber-Bosch process to prod fertilizer. #ASM2014
8:45am May 18th 2014 via Hootsuite
Schleper: Figure from http://t.co/Qms5Vtn6uf review on phylogeny of ammonia oxidizing archaea; enrichments & pure cultures #ASM2014
8:41am May 18th 2014 via Hootsuite
Schleper: Even found in clean rooms at NASA, wastewater plants, on leaves #ASM2014
8:39am May 18th 2014 via Hootsuite
Schleper: Ammonia oxidation important in the nitrogen cycle, now 'massively studied' Thaumarchaeota found all over #ASM2014
8:38am May 18th 2014 via Hootsuite
Schleper: Not so many model org's for coastal marine plantktonic archaea. Thaumarchaeota, in 2005 Cenarchaeum ammonium oxidation #ASM2014
8:37am May 18th 2014 via Hootsuite
Schleper: Photo of bachelor students collecting hot spring samples and going to HT sequencing for analysis #ASM2014
8:35am May 18th 2014 via Hootsuite
Schleper: Many trained in southern Germany; photo in 2005 Bavaria - going outside E. coli #ASM2014
Schleper: Discovering unusual life forms, rule breakers, and dispelling dobmas. 100C, extremely low pH, virus changing shape #ASM2014
8:34am May 18th 2014 via Hootsuite
Schleper: RNA polymerases from Archaea isolated from Iceland - where it all began. "I found it rather boring" when first began work #ASM2014
8:32am May 18th 2014 via Hootsuite
Schleper: Two Germans inspired by Woese: Zillig (Munich) and Stetter (Regensburg) the 'first generation' #ASM2014
8:31am May 18th 2014 via Hootsuite
Schleper: "No one could imagine that there could be anything microbial that was not phylogenetically a 'eubacteria'" Woese #ASM2014
8:29am May 18th 2014 via Hootsuite
Schleper: Has been working with Archaea for 25y. From communities, sulfobales, CRISPR-virus defens, methanogens... #ASM2014
Up next: Christa Schleper, Univ Vienna, "The Archaea Among Us: Thaumarchaeota and Others that Affect Our Daily Life" #ASM2014
8:27am May 18th 2014 via Hootsuite
Pace: 'Heterotachy' is a variable rate of change among sequences "a statistical killer" causing problems with phylogeny #ASM2014
8:24am May 18th 2014 via Hootsuite
Pace: Crenarchaeota, Euryarchaeota 'split' goes deep; different opposing models Lake 1988 'Eocyte' model; Embley 2008 model #ASM2014
8:17am May 18th 2014 via Hootsuite
Pace: Found 3 domains but 'no root' until 1989 and PNAS 1990 PubMed http://t.co/zY1DC4prK0 divided Archaea into 2 #ASM2014
8:15am May 18th 2014 via Hootsuite
Pace: 'six-mer or longer' has low random probabilty: used as homologues, common ancestry, infer relationships. 16S rRNA #ASM2014
8:13am May 18th 2014 via Hootsuite
Pace: 2D electrophoresis of 32P-labeled oligos - small subunit phylogeny, photo of Carl studying x-ray autorads. Before sequencing #ASM2014
8:12am May 18th 2014 via Hootsuite
Pace: PNAS paper: http://t.co/PmwjDxzKlx The only data was arcane (without sequences). Showed 2D fingerprint of RNAse T1 digest #ASM2014
8:11am May 18th 2014 via Hootsuite
Plenary at #ASM2014: "A Tribute to Carl Woese", Norman Pace (UC Boulder) "Carl Woese: Legacy, Archaea and a New Microbiology"
8:08am May 18th 2014 via Hootsuite
Plenary at #ASM2014:
8:07am May 18th 2014 via Hootsuite
Interesting how an #ASM2014 app makes you feel super-busy on a Sunday morning. http://t.co/pnwSCw8NVp
7:47am May 18th 2014 via Hootsuite
RT @markowenmartin: Microbes on the big screen! Biology in Boston happening now #asm2014 http://t.co/XgGCIlnJF1
7:37am May 18th 2014 via Hootsuite
Warning to twitter followers: please put me on mute if you don’t want to hear about #ASM2014 the next 3d... http://t.co/nQLhtaYCLC
7:35am May 18th 2014 via Hootsuite
Interesting resume experiment: the college summers made the difference | WSJ http://t.co/hYah13Lt27
9:08pm May 17th 2014 via Hootsuite
RT @Eukaryotes: Hmmm... back cover of #ASM2014 program... http://t.co/MLjL6SJspz
6:40pm May 17th 2014 via Hootsuite
RT @rvaughnmd: Ground breaking hip and stem cell surgery completed using 3D printed implant | Science Daily http://t.co/GeqSbpchdU
5:35pm May 17th 2014 via Hootsuite
Headed to #ESHG14? The abstracts are now 'live' with an itinerary planner too. http://t.co/vbZ17aT1y6
4:35pm May 17th 2014 via Hootsuite
.@kennamshaw Pre-check has been such a godsend for me too! (MCO for business is always such a contrast with all the Mickey ears...)
3:48pm May 17th 2014 via Hootsuite in reply to
