Joachim Messing (Rutgers Univ) (yes, >that< Messing): “Sequencing Long DNA Molecules and Modifying Them” #ASM2014 http://t.co/Q9RSGvr8

8:05am May 20th 2014 via Hootsuite

.@iontorrent PGM and 16S at #ASM2014 noon in the Product Theater http://t.co/57HnFOG9aF

7:36am May 20th 2014 via Hootsuite

RT @federicacocco: Independent article how the internet is changing journalism doesn't have a single link in it. :( http://t.co/LoTXKM1sc3

7:10am May 20th 2014 via Hootsuite

.@archaeal @kcwrighton Thanks for the reference!

5:59am May 20th 2014 via Hootsuite in reply to

.@drgitlin Yesterday with NASA folks at #ASM2014 I discovered there was a difference in stds for 'robotic' vs. 'human-weighted craft'

5:58am May 20th 2014 via Hootsuite in reply to

RT @kareynlo: Storified tweets #asm2014: Jo Handelsman & Graham Hatfull about SWI and SEA-PHAGES programs. http://t.co/lXbe5y0HeB

10:45pm May 19th 2014 via Hootsuite

RT @ARKgenome: Dogs can detect prostate cancer with 98% accuracy: http://t.co/0J1a986Rkf Maybe this will help develop new #MDx.

8:25pm May 19th 2014 via Hootsuite in reply to

Reported at #ASM2014 @phylo_game: Microbial stowaways to Mars identified http://t.co/QMGS9hmIHl

6:58pm May 19th 2014 via Hootsuite

RT @scopedbylarry: CDC developed MERS diagnostic kits now dispatched to 41 countries around the world #asm2014

6:40pm May 19th 2014 via Hootsuite

.@kareynlo ...the first part of the session due to an overly-long conference call!

6:22pm May 19th 2014 via Hootsuite in reply to

.@kareynlo thanks for putting the Storify together. I regret missing

6:21pm May 19th 2014 via Hootsuite in reply to

Glaven: Fe oxidizing bact. for electron xfer at electrode: looking at Chromatiaceae, others #ASM2014

3:48pm May 19th 2014 via Hootsuite

Glaven: Only 16-17 genome clusters of the biofilm; publication in prep. #ASM2014

3:42pm May 19th 2014 via Hootsuite

Glaven: Naval research lab: proteomics work was most informative. Electrode biofilm was communicated via protein #ASM2014

3:39pm May 19th 2014 via Hootsuite

Glaven: Biofilm enriched 5y.o. from seawater; low diversity, stable, lives on electrode, CO2 and O2. 2013 http://t.co/lVRNBF30DN #ASM2014

3:37pm May 19th 2014 via Hootsuite

Glaven: A biocathode - single organisms live on the opposite side, drawing electrons out, microbes acting as catalysts #ASM2014

3:34pm May 19th 2014 via Hootsuite

Sarah Strycharz-Glaven: Electric Marine Biofilms For Bioenergy: Systems Approach To Drive Synthetic Biology Of Microbial Consortia #ASM2014

3:33pm May 19th 2014 via Hootsuite

Jin: Co-fermentation of cellobiose + xylose, presented figure from this 2011 PNAS http://t.co/0CKvi2GBvT #ASM2014

3:16pm May 19th 2014 via Hootsuite

Jin: If glucose + xylose together in fermentation, glucose preferred source. Described in 2009 PNAS http://t.co/HoLTrAHbiL #ASM2014

3:13pm May 19th 2014 via Hootsuite

Jin: Cloning 3 enzymes from Pichia to breakdown xylose; many iterations to increase yield PLOS One: http://t.co/b7kqzSHVjT #ASM2014

3:10pm May 19th 2014 via Hootsuite

Jin: S. cerevisiae: long history and many tools but can't use xylose; not suitable for cellulosic fermentation #ASM2014

3:07pm May 19th 2014 via Hootsuite

Jin: Sugar fermentation from corn glucose different than cellulosic biomass gives glucose, xylose + acetate, mixed ferment. #ASM2014

3:06pm May 19th 2014 via Hootsuite

Yong-su Jin (Univ IL at Urbana-Champaign): Synthetic Biological Widgets: Engineered Yeasts for Producing Fuels and Chemicals #ASM2014

3:02pm May 19th 2014 via Hootsuite

Bistué: Q: orientation matter for s10? A: Had to keep orientation colinear with the origin of replication to prevent collision #ASM2014

3:00pm May 19th 2014 via Hootsuite

Bistué: Of 4 isogenic strains, further away = slower replication (showed video evidence). Via qPCR: lower gene dosage #ASM2014

2:55pm May 19th 2014 via Hootsuite

Bistué: Project to change S10 location within V cholerae genome, even on secondary chromosome #ASM2014

2:52pm May 19th 2014 via Hootsuite

Bistué: V. cholerae a fast-grower, genes close to oriC1; ribosomal genes very close to s10 locus. Conserved universally #ASM2014

2:51pm May 19th 2014 via Hootsuite

Bistué: Obsv: fast-growing bact. have genes closer to replication origin, increasing gene dosage #ASM2014

2:50pm May 19th 2014 via Hootsuite

Bistué: Synthetic biology won't advance until genome organization / chromosome structure is better understood #ASM2014

2:49pm May 19th 2014 via Hootsuite

A. Soler-Bistué: s10-spec-α Repositioning in Vibrio cholerae Provides Insight into Genome Organization Rules of Fast-Growers #ASM2014

2:48pm May 19th 2014 via Hootsuite

RT @JesusAlvelo: Lots of great microbiology from across the globe at the #ASM2014. Loving the internationalization of the conference.

2:46pm May 19th 2014 via Hootsuite

Beisel: Used E.coli, promoter upstream of GFP, minimal M9 media, look at cells via flow cytometry. #ASM2014

2:36pm May 19th 2014 via Hootsuite

Beisel: Go back to the lac operon - positive feedback, an all-or-none response as a model of sugar utilization #ASM2014

2:34pm May 19th 2014 via Hootsuite

Chase L. Beisel: "Diverse Single-Cell Behaviors in Microbial Sugar Utilization" #ASM2014

2:33pm May 19th 2014 via Hootsuite

Handelsman: Bring microbiol. to students of all types; credits to Helmsley Charitable Trust, HHMI, Yale, and ASM #ASM2014

1:35pm May 19th 2014 via Hootsuite

Handelsman: Expanding: going int'l, also e-training this summer to scale. Next is chemical analysis; open access to materials #ASM2014

1:34pm May 19th 2014 via Hootsuite

Handelsman: Much higher completion rate in the SWI group with FL students #ASM2014

1:30pm May 19th 2014 via Hootsuite

Handelsman: One example from State Coll. of FL: Eric Warrick did a shared lecture format, Trad. vs. SWI labs. Meas. improvement #ASM2014

1:29pm May 19th 2014 via Hootsuite

Handelsman: 900 students from Spring 2014; 4-7w compressed format; student posters ended up here at this meeting #ASM2014

1:28pm May 19th 2014 via Hootsuite

Handelsman: Spring 2014 26 institutions launched courses; variations on content to enrich protocols; tested tools / resources #ASM2014

1:27pm May 19th 2014 via Hootsuite

Handelsman: 6d 2013 Summer workshop, lab exp., pedagogical principles, design methods, courses to implement #ASM2014

1:26pm May 19th 2014 via Hootsuite

Handelsman: A Siberian student interacts with a Yale one. SWI pilot partners across the US map shown #ASM2014

1:25pm May 19th 2014 via Hootsuite

Handelsman: Small World Initiative (SWI): the potential to inspire to solve a real global problem. Engagement & network & interact #

1:24pm May 19th 2014 via Hootsuite

Handelsman: Choose an environmental sample, isolate and prioritize microbes, char organism, extract antibiotics, purify & char #ASM2014

1:23pm May 19th 2014 via Hootsuite

Handelsman: New habitats; small world is crowdsourcing discovery. Put the problem of antibiotic discovery, let freshmen solve it #ASM2014

1:22pm May 19th 2014 via Hootsuite

Handelsman: Antibiotic development: 75% come from soil bacteria; 100K natural products from Streptomyces spp #ASM2014

1:21pm May 19th 2014 via Hootsuite

Handelsman: MRSA: 64% mortality; 15x increase in mortality since 1993 #ASM2014

1:19pm May 19th 2014 via Hootsuite

Jo Handelsman (Yale): "The Small World Initiative: A Global Community of Undergraduate Researchers" #ASM2014

1:18pm May 19th 2014 via Hootsuite

RT @YinzMicrobes: Go to http://t.co/7cwgGoDn6O now for Fall 2015 SEA-PHAGES cohort applications #asm2014

1:16pm May 19th 2014 via Hootsuite