Hatfull: Annual symposium at HHMI Janelia Farm photo; application for Fall 2015 here http://t.co/wsyASFNxNC #ASM2014
1:15pm May 19th 2014 via Hootsuite
MT @shannonegreene: Hatfull: 2068 freshmen in SEA-PHAGE project with 6800 touched overall. Future @ASMicrobiology members! #asm2014
1:13pm May 19th 2014 via Hootsuite
RT @shannonegreene: Love it! Seeing phage names like "twister" and " barnyard." Science isn't all capital letters and numbers ;) #asm2014
A cool @BioFireDX ‘fire’ on the #ASM2014 exhibit hall. http://t.co/hGWgkuoSMl
12:21pm May 19th 2014 via Hootsuite
Wrighton: Instead of Fe reduction, a much different model for acetate; diagram in this ISME pub: http://t.co/Z0wDyM8ukE #ASM2014
9:55am May 19th 2014 via Hootsuite
Wrighton: ACD20 also observed in gut in this 2013 pub: http://t.co/wfJVE6y8UQ #ASM2014
9:53am May 19th 2014 via Hootsuite
Wrighton: aa auxotrophy, stable consortia, "I suspect many have a hydrogen economy" that requires a consortia of organisms #ASM2014
9:51am May 19th 2014 via Hootsuite
Wrighton: Looked at <0.2um samples that are 'division candidates'. Hypotheses: small size, G+-like walls, hydrogen economy #ASM2014
9:50am May 19th 2014 via Hootsuite
Wrighton: Pointed to this paper Nature 2013 that Tanya Woyke pointed to just an hour ago: http://t.co/n6dInwSZAC #ASM2014
9:48am May 19th 2014 via Hootsuite
Wrighton: 'Archaeal-like sulf-hydrogenase', still needs verification. #ASM2014
9:47am May 19th 2014 via Hootsuite
Wrighton: No cell wall, didn't find any sulfur, nor electron transport chain, expressing protein in ecosystem #ASM2014
9:46am May 19th 2014 via Hootsuite
Wrighton: 10 near-complete OD1 genomes; notable have small genome sizes (0.7 - 1.2Mb). Plot by GC. "No the cells aren't purple" #ASM2014
9:45am May 19th 2014 via Hootsuite
Wrighton: Presented the superphyla in this 2012 Science paper: http://t.co/7dMIEMmwII #ASM2014
9:43am May 19th 2014 via Hootsuite
Wrighton: Recovered 81 genomic bins, 74 bacteria, no archaea; 16S superphylum identified #ASM2014
9:42am May 19th 2014 via Hootsuite
Wrighton: Went to uranium ore mine tailing site, bioremediation effort (Old Rifle site) Geobacter spp, effect of acetate on system #ASM2014
9:40am May 19th 2014 via Hootsuite
Wrighton: (she's @kcwrighton ) Dark matter defined: what we know little about. Taxonomy fig Baker Dick 2013 http://t.co/0zRgyBKcVi #ASM2014
9:37am May 19th 2014 via Hootsuite
Kelly Wrighton (Ohio State): Illuminating Microbial Dark Matters: New Ecosystem Roles for Uncultivated Bacterial Phyla #ASM2014
9:33am May 19th 2014 via Hootsuite
Brady: Q: Efficiency of expression of clones? A: A majority, 50-60% with a standard promoter #ASM2014
9:32am May 19th 2014 via Hootsuite
@danudwary @bioinformer Interested in your reply. (Standing on my head didn't help.) :o)
9:31am May 19th 2014 via Hootsuite in reply to
Brady: eSNaPD software available online http://t.co/MDvus6C5lL Lab website: http://t.co/E6RhwxkAy1 #ASM2014
9:29am May 19th 2014 via Hootsuite
Brady: Shows Tetarimycin A, active against MRSA. Publication in J ACS 2013 here: http://t.co/cfXtZihmJ7 #ASM2014
9:27am May 19th 2014 via Hootsuite
Brady: Isolated aromatic polyketides: can show phylogeny and evolutionary ancestor. #ASM2014
9:26am May 19th 2014 via Hootsuite
.@danudwary Got to love rhetorical questions during presentations - the answer is usually yes...
9:25am May 19th 2014 via Hootsuite in reply to
Brady: Novel sequences: ~98% have no close relatives. Can we use a clade-based strategy instead? #ASM2014
9:23am May 19th 2014 via Hootsuite
Brady: Antracyclines (anti-cancer agent) can look for sequence tags in their libraries, sequence, express; assay (Arimetamycin A) #ASM2014
9:22am May 19th 2014 via Hootsuite
Brady: Is tracing chemical properties with geography, in the environment. Resistance mechanisms, promising leads #ASM2014
9:20am May 19th 2014 via Hootsuite
Brady: Some of this work appeared in his 2013 PNAS paper here: http://t.co/CAuvLwqHzH #ASM2014
9:17am May 19th 2014 via Hootsuite
Brady: Can go back to clone well to sequence the clone. 80% of the time the 400bp fragment works #ASM2014
9:14am May 19th 2014 via Hootsuite
Brady: First six samples: found 444 seq tags from TX soil, new erythromycin etc. Believable? 400bp from 100kb pathway? #ASM2014
9:13am May 19th 2014 via Hootsuite
Brady: Like with 16S - look for ketosynthase domains, adenylation domains #ASM2014
9:12am May 19th 2014 via Hootsuite
Brady: Natural product biosynth - only 6 major pathways with incredible diversity #ASM2014
9:11am May 19th 2014 via Hootsuite
Brady: Probe the sequences for novel chemistry: new glycopeptide biosynthetic pathways #ASM2014
9:09am May 19th 2014 via Hootsuite
Brady: Now: capture HMW DNA, make cosmids, 10M-20M clone 'mega libraries'. The phenotype screening also problematic #ASM2014
9:07am May 19th 2014 via Hootsuite
Brady: Goal is to find new natural products (small molecules). But starts with culture (only 1%), after culturing ~10-20% genes #ASM2014
9:06am May 19th 2014 via Hootsuite
Sean Brady (Rockefeller Univ): "Watch Where You Step, There Is New Chemistry Everywhere" #ASM2014
9:03am May 19th 2014 via Hootsuite
RT @microbegrrl: Rosenberg: “Sometimes stochastic only really means that we don’t know how it works” #asm2014
9:02am May 19th 2014 via Hootsuite
Hatzenpichler: Can also enrich for newly synth proteins: use alkyne-labeled biotin tags; affinity chromotography; prot detect #ASM2014
9:01am May 19th 2014 via Hootsuite
Hatzenpichler: Future directions include use of L-Homopropargyglycine (HPG) as another Met substitute; also sort based on activity #ASM2014
8:58am May 19th 2014 via Hootsuite
Hatzenpichler: Plot of Met content per predicted proteome; stark difference between archaea and bact. Few have zero Met #ASM2014
8:47am May 19th 2014 via Hootsuite
Hatzenpichler: Shows no effect on growth rate, protein updake w/AHA; data from this 2014 paper http://t.co/WEeThrPCfY #ASM2014
8:43am May 19th 2014 via Hootsuite
RT @JimDunnIt1: Oooo using an analog of Met with an azide group to tag proteins during synthesis, combining biochemistry and microbio...
8:41am May 19th 2014 via Hootsuite
Hatzenpichler: AHA has minimal structural disturbance, non-toxic to euk, bact. and archaea. Only one azide-containing biomolecule #ASM2014
Hatzenpichler: Azidohomoalanine (Aha, subst for Met) for uncultured organisms About 390:1 activation rate Met:Aha #ASM2014
8:40am May 19th 2014 via Hootsuite
Hatzenpichler: Azide with an alkyne group; explanation here (Wikipedia) http://t.co/OF2DsKRFH2 Studies used for pathogens #ASM2014
8:39am May 19th 2014 via Hootsuite
Hatzenpichler: AA such as Se-Met, Aha, Hpg, Hag for naturally-occuring Met. Click chemistry: a name for sth. done for decades #ASM2014
8:37am May 19th 2014 via Hootsuite
Hatzenpichler: Propose Bioorthogonal non-canonical amino acid tagging (Boncaat). Uses translational promiscuity for unnatural aa #ASM2014
8:36am May 19th 2014 via Hootsuite
Hatzenpichler: At the single-cell level, what about regulatory, structural proteins, metabolic components? Over time? #ASM2014
8:35am May 19th 2014 via Hootsuite
Roland Hatzenpichler (CalTech): "Click Chemistry Mediated Protein Tagging: A New Approach for Studying Microbial in situ Activity" #ASM2014
8:34am May 19th 2014 via Hootsuite
Woyke: Q: Why could they not be cultivated? A: Other collaborators are working on it #ASM2014
8:28am May 19th 2014 via Hootsuite
Woyke: Plan also a microbial dark matter project II; looking for sites enriched for candidate phyla #ASM2014
8:27am May 19th 2014 via Hootsuite
