September 2014

Dowds: Reviewing Ion Reporter to filter out 50K variants from an exome to 12, and then down to details on each one #IWT14

6:01am September 15th 2014 via Hootsuite

Dowds: Also in Torrent Suite 4.2.1: multi-run summary view; can run as virtual machine to try new versions, try plug-ins #IWT14

5:55am September 15th 2014 via Hootsuite

Dowds: Now Torrent Suite run-plans with template ability; runs and data tab shows list of reports, now ability to filter here #IWT14

5:53am September 15th 2014 via Hootsuite

Dowds: Showed demo movie of an http://t.co/6puEapf9xO design, v. straightforward process. (Much more interesting than static figs) #IWT14

5:48am September 15th 2014 via Hootsuite

Dowds: Overview: design via http://t.co/6puEapf9xO, Torrent Suite software, and then Ion Reporter for filtering, reporting #IWT14

5:44am September 15th 2014 via Hootsuite

Next: Carl Dowds (Thermo Fisher Scientific) "Ion bioinformatics latest tools and updates" #IWT14

5:40am September 15th 2014 via Hootsuite

Affourtit: Q: for lincRNA? A: For >125bp, fine. Can be done at http://t.co/YMIeplhn73 can just put in gene name to see. #IWT14

5:39am September 15th 2014 via Hootsuite

Affourtit:Q:AmpliSeq Transcriptome for alt splicing? A:Yes - can do isoform-spec panel for AS RNA, quantitatively. #IWT14

5:38am September 15th 2014 via Hootsuite

Affourtit: PGM Dx will have important templating (OneTouch2) in-process control #IWT14

5:37am September 15th 2014 via Hootsuite

Affourtit: Mentioned availability of new long mate-pair kit called TrueMate (Behind the Bench blog here http://t.co/P4NiRINw2S ) #IWT14

5:36am September 15th 2014 via Hootsuite

Affourtit: (More about AmpliSeq Transcriptome on the Behind the Bench blog: http://t.co/eU1HZ3liSb ) #IWT14

5:34am September 15th 2014 via Hootsuite

Affourtit: AmpliSeq Transcriptome has correlation R^2 0.96 with TaqMan; 0.94 with RNA-Seq; Microarray = 0.87 #IWT14

5:30am September 15th 2014 via Hootsuite

Affourtit: 10ng input FFPE-compat. RNA input; 'people use quite a bit less than that'; multiple isoforms from a single primer pair #IWT14

5:29am September 15th 2014 via Hootsuite

Affourtit: New product AmpliSeq Transcriptome: scaling AmpliSeq RNA to >20K genes. Diff gene exp to RefSeq in one tube #IWT14

5:28am September 15th 2014 via Hootsuite

Affourtit: PII expected to go into early access before end of the year. ISPs and well geometry locked; RNA-Seq on PII has R=0.98 #IWT14

5:25am September 15th 2014 via Hootsuite

Affourtit: Proton PI v3 is current kit. 2 runs per init now supported on TS 4.2.1; user bulletin on Ion Comm. http://t.co/5XUCH70Fxz #IWT14

5:21am September 15th 2014 via Hootsuite

Affourtit: Hi-Q PGM data on Ion Chef: 200 and 400bp PGM sequencing both loading and yield improve #IWT14

5:19am September 15th 2014 via Hootsuite

Affourtit: Hi-Q on Proton is Q1 2015; on Ion AmpliSeq Exome indel FP rate 50% reduced (to 2 per megabase) #IWT14

5:17am September 15th 2014 via Hootsuite

Affourtit: Q4 2014 will have new Hi-Q template kit. Can move seq kit to HiQ now, template kit change before end of year #IWT14

5:17am September 15th 2014 via Hootsuite

Affourtit: Showed 2300 amplicon panel, 43% reduction in indel rate; HP in size from 1 to 14bp. Another panel no change of indel error #IWT14

5:15am September 15th 2014 via Hootsuite

Affourtit: Showed Rhodococcus palusris data, marked improvement 'Q>30 thru the 400bp read'. 78% - 94% reduction of indel error #IWT14

5:14am September 15th 2014 via Hootsuite

Affourtit: HiQ on PGM now has one kit (200 & 4000). Indel error reduction for better de novo work; equivalent or better for targeted #IW

5:12am September 15th 2014 via Hootsuite

Affourtit: PGM Hi-Q on OT2, TS 4.2.1 now; Hi-Q on Proton on OT2 before end of 2014, TS 4.4; Hi-Q on Ion Chef + PGM before end of year #IWT14

5:10am September 15th 2014 via Hootsuite

Affourtit: Publications review: almost 800; freq publ. topic is targeted DNA (almost 300). 2 focus areas: accuracy & workflow #IWT14

5:08am September 15th 2014 via Hootsuite

Jason Affortit (Thermo Fisher Scientific), @topoisomerase : "State of the art of Ion Torrent™ technology" #IWT14

5:04am September 15th 2014 via Hootsuite

Here at Ion World Berlin, will be live-tweeting under #IWT14 today and tomorrow. First: @topoisomerase of @iontorrent sharing an update talk

4:56am September 15th 2014 via Hootsuite

Don’t Take Your Vitamins | FiveThirtyEight http://t.co/r7eS46z3vJ

4:00am September 15th 2014 via Hootsuite

How China’s Great Firewall works http://t.co/p9dLhwQjhV

4:00am September 14th 2014 via Hootsuite

RT @iontorrent: NGS profiling lung cancer FFPE samples shows high concordance with Sanger sequencing, IDs mut & CNVs: http://t.co/ZT93zD

4:00am September 13th 2014 via Hootsuite

RT @drbachinsky: Genomic distribution and possible functions of DNA hydroxymethylation in the brain http://t.co/zsbGHzHhHv

10:00pm September 12th 2014 via Hootsuite

UTSW RT @JohnCFierce: ICYMI: Google's stealthy Calico inks R&D deal for new compounds aimed at neurodegeneration http://t.co/eMxWO84cyq

9:00pm September 12th 2014 via Hootsuite

RT @ryneches: Woah. A woman lived 24 years until someone noticed she was missing her whole cerebellum | New Scientist http://t.co/Kr19eeBKeV

8:00pm September 12th 2014 via Hootsuite

RT @TheEconomist: A study shows that being dyslexic in Chinese is not the same as being dyslexic in English http://t.co/DJe8aPy3t3

6:00pm September 12th 2014 via Hootsuite

Discovery of new natural antibiotics RT @Sciwhat: This bit on the human #microbiome is fascinating: http://t.co/dRXVgk5OgS

5:01pm September 12th 2014 via Hootsuite

RT @NatureNews: NASA to return sample from Earth-bound asteroid http://t.co/EnzykeN0Z1

4:01pm September 12th 2014 via Hootsuite

RT @noordinaryco: Rapid DNA sequencing from a simple laptop and the future of technology | Medium http://t.co/xEINpe6DLt #technology

3:01pm September 12th 2014 via Hootsuite

RT @InSequence: Oxford Nanopore Presents Details on New High-throughput Sequencer, Improvements to MinIon http://t.co/tI5g5xt5Ii

2:01pm September 12th 2014 via Hootsuite

Mt. Sinai to Open NGS Facility, Initially Equip with Ion Torrent Platforms | GenomeWeb http://t.co/kXeifWN4xW

1:05pm September 12th 2014 via Hootsuite

RT @iontorrent: The Ion Proton will be deployed in support of Mount Sinai’s Resilience Project http://t.co/stkqIcoerg http://t.co/wsLSR6zKKi

1:00pm September 12th 2014 via Hootsuite

:o) RT @mdshw5: @notSoJunkDNA @BioMickWatson SNV = one nutcase, SNP = a population frequency of nutcases.

12:01pm September 12th 2014 via Hootsuite

@neilfws: This is really good. "10 simple rules for better figures": http://t.co/6lJBP3k957

11:01am September 12th 2014 via Hootsuite

RT @EricTopol: The velocity of change and impact of mobile devices is striking http://t.co/5l8UGlKttP @TheEconomist http://t.co/eooRb6L4hS

10:01am September 12th 2014 via Hootsuite

NIH Director: China Leads U.S. in Genomic Research | Health Data Management http://t.co/VQpFTFkrgZ

9:01am September 12th 2014 via Hootsuite

Ion World Tour #IWT14 Manchester Day 2: Andy Felton giving an Ion PGM Dx update http://t.co/nhodTTuSrZ

8:07am September 12th 2014 via Hootsuite

RT @DNABARCODE: Angelina Jolie’s Surgery May Have Doubled #Genetic Testing at One Clinic - http://t.co/7X2vsFVmpg

8:01am September 12th 2014 via Hootsuite

Tonge: Plans to not only do bacterial but also fungal metagenomics on these 'green wall' plants for high vs low VOC consuming variety #IWT14

7:19am September 12th 2014 via Hootsuite

Tonge: Green walls "Rhizosphere microbiome" - can detoxify environmental volatiles. Root zone has microbiome #IWT14

7:18am September 12th 2014 via Hootsuite

Tonge: Conclude: qualitative (due to copy number), not a viability test (although work underway) #IWT14

7:16am September 12th 2014 via Hootsuite

Tonge: Now 20 spores, many with very close similarities (same genus). All cases species called #IWT14

7:15am September 12th 2014 via Hootsuite