The Starbucks Cup Dilemma | Fast Company http://t.co/3fzZgZfBjz
5:14am September 18th 2014 via Hootsuite
RT @TheAtlantic: Left behind: About 70 percent of U.S. elementary schools still rely on slow Internet connections http://t.co/QP2ka2b5bF
9:05pm September 17th 2014 via Hootsuite in reply to
RT @AAAS_News: The Eruptions of Iceland’s Bardarbunga Volcano (via @in_focus) http://t.co/OdYVm5szlc
8:05pm September 17th 2014 via Hootsuite in reply to
RT @bioitworld: The Latest Scoop on the MinION Portable Sequencer http://t.co/Hl0uMGtqWR
7:05pm September 17th 2014 via Hootsuite in reply to
RT @AnnTran_: At these tech companies, the biggest office perk is getting rid of the office: http://t.co/7UNeNL87lp via @qz
6:05pm September 17th 2014 via Hootsuite in reply to
RT @washingtonpost: China’s president to Chinese travelers: Please eat fewer instant noodles while abroad http://t.co/p68I7P69xb
5:05pm September 17th 2014 via Hootsuite in reply to
RT @Gizmodo: Don’t put iOS 8 on your iPhone 4S: http://t.co/N0juQyu9m2 http://t.co/mjOkW8HieL
4:05pm September 17th 2014 via Hootsuite in reply to
MT @trueanomalies: “top 50 science stars of Twitter”; thinking “wow that’s a lot of white men” http://t.co/vskEKY8Kp3 http://t.co/A0CWroeH04
3:01pm September 17th 2014 via Hootsuite in reply to
RT @engadget: In case you missed it last night, here’s our iPhone 6 / iPhone 6 Plus review: http://t.co/bCARJkfJ5h http://t.co/C03GWVaYzF
2:01pm September 17th 2014 via Hootsuite in reply to
RT @scicurious: Interesting @cragcrest post on the pros and cons of the treadmill desk http://t.co/fgZ0sqkeYN Shamed me into standing up
1:01pm September 17th 2014 via Hootsuite in reply to
Good advice RT @DeniseNatishan: 5 Secrets of the Guy Who Gets Multiple Job Offers https://t.co/0O5RnryHFk via @dailymuse
12:05pm September 17th 2014 via Hootsuite in reply to
RT @waltmossberg: Which new iPhone is best for you? I explain in my review. http://t.co/7OlwqJEA16.
11:05am September 17th 2014 via Hootsuite in reply to
#longread RT @IATV: “Nature vs. Smartphones” http://t.co/dAMGB3xtL4 (http://t.co/v1PgolEe4G)
10:05am September 17th 2014 via Hootsuite in reply to
RT @iontorrent: RNA-seq of iPSCs reveals molecular synaptic defects due to a familial schizophrenia mutation: http://t.co/brk6i7wYmH
9:05am September 17th 2014 via Hootsuite in reply to
RT @gigaom: What the early reviews are saying about the iPhone 6 http://t.co/AHXrC2f2FL
8:05am September 17th 2014 via Hootsuite in reply to
RT @SnoozeInBrief: Worst. Exercise plan. Ever. #indyref http://t.co/rLo30uMrEj
7:07am September 17th 2014 via Hootsuite in reply to
RT @tnhh: Too Few University Jobs For America’s Young Scientists http://t.co/t1bUqj5rar
6:14am September 17th 2014 via Hootsuite in reply to
RT @westr: #10. Hell Engineering: RT @erikailves: 10 Horrifying Technologies That Should Never Be Allowed To Exist http://t.co/kk1VV4gkJf
4:08am September 17th 2014 via Hootsuite in reply to
RT @HuffPostCollege: Americans are saving more than ever for college http://t.co/XrdZLOt2C7
3:05am September 17th 2014 via Hootsuite
RT @iontorrent: RNA-seq of iPSCs reveals underpinnings of synaptic defects due to a familial schizophrenia mutation: http://t.co/brk6i7wYmH
2:05am September 17th 2014 via Hootsuite
RT @nytimesscience: There are as many as 4,500 clinical trials involving stem cells underway in the U.S. right now http://t.co/Ar1vL8wc0V
1:05am September 17th 2014 via Hootsuite
MT @iontorrent: Human populations have grown since the Stone Age, so have the # of rare disease-related genetic var's http://t.co/7l4C9a1KT7
12:05am September 17th 2014 via Hootsuite
Physicians' Genomics Knowledge, Basic Skills Still Barrier to Adoption | GenomeWeb http://t.co/TjZFmtVNM0
11:00pm September 16th 2014 via Hootsuite
Behind the Bench: Fishing for Rare Pathogenic De Novo Variants in a Sea of SNVs http://t.co/hQThITT14m
10:00pm September 16th 2014 via Hootsuite
New post: Human Identification by NGS | Behind the Bench http://t.co/V5nSvrFm9A
9:00pm September 16th 2014 via Hootsuite
The missing piece to changing the university culture : Nature Biotech http://t.co/GN3WFnDJGP A depressing figure of PhDs vs. acad. positions
8:01pm September 16th 2014 via Hootsuite
RT @SwedishCanary: This salad tastes like I'd rather be fat.
7:00pm September 16th 2014 via Hootsuite
MT @sritchie73: Lee Hood advocating a holy trinity: biology, technology and computation. Seems to miss the final key: statistics #icsb2014
6:00pm September 16th 2014 via Hootsuite
Almost one-half of wealthy Chinese plan to leave | WSJ http://t.co/oURAy5KiNY
11:02am September 16th 2014 via Hootsuite
Vanhatalo: (Points out why the usable key signal is only 3 bp) #IWT14
10:11am September 16th 2014 via Hootsuite
Vanhatalo: Ion Chef library amts range from 50-100pM, 5x more than the rec'd 11pM for OT2 #IWT14
9:59am September 16th 2014 via Hootsuite
Vanhatalo: Qubit, BioAnalyzer, qPCR all good for library quant. No to nanodrop or a gel - not helpful #IWT14
9:56am September 16th 2014 via Hootsuite
Vanhatalo: "Covaris and Biorupter have less variability across sample types, compared to enzymatic methods" #IWT14
9:52am September 16th 2014 via Hootsuite
Vanhatalo: Qualifying starting DNA with RNAseP real-time PCR (document on Ion Community: http://t.co/tS5LKrBgYf ) #IWT14
9:47am September 16th 2014 via Hootsuite
Vanhatalo: Best practices document at Ion Community (free reg) http://t.co/K7OwkAifOW #IWT14
9:43am September 16th 2014 via Hootsuite
Last: Jussi Vanhatalo (Thermo Fisher Scientific): "Best practices, quality control along the Ion Workflow and more" #IWT14
9:42am September 16th 2014 via Hootsuite
Tyrelle:Q:What is common signal shift? A:The key sequences, shifted up (or down) along the read, a parameter #IWT14
9:33am September 16th 2014 via Hootsuite
Tyrelle: Alllele=no call: hotspot DEL filtered due to 'common signal shift'. Can dig back in and look at how close to threshold #IWT14
9:30am September 16th 2014 via Hootsuite
Tyrelle: Source=hotspot, Type=MNP: a hotspot file generated. Contrast: missing SNV in hotspot. 0.0% freq. 3rd: not-called novel Del #IWT14
9:27am September 16th 2014 via Hootsuite
Tyrelle: In essence, going back to data the bases were called originally, now in the context of all the other signals at that base #IWT14
9:19am September 16th 2014 via Hootsuite
Tyrelle: TVC looks at the underlying raw data; signal properties in the aggregate examined (these are flowspace signals) #IWT14
9:18am September 16th 2014 via Hootsuite
Tyrelle: De-amination metric - not filtered on, just a parameter for FFPE or other samples where effect can be seen #IWT14
9:16am September 16th 2014 via Hootsuite
Tyrelle: MNP=multi-nucleotide polymorphism. COMPLEX=block-substitutions. #IWT14
9:14am September 16th 2014 via Hootsuite
Tyrelle: TVC (Torrent Variant Caller) is available at GitHub under GPLv2 located here: http://t.co/MEERCfqf7y #IWT14
9:13am September 16th 2014 via Hootsuite
Greg Tyrelle (Thermo Fisher Scientific) "Variant Calling, Mutation Analysis, and Data Interpretation" #IWT14
9:12am September 16th 2014 via Hootsuite
Sartori: 'Further developments for Hematology currently under evaluation'; open for discussion and input for the next project #IWT14
9:01am September 16th 2014 via Hootsuite
Sartori: Can call most mutations, still needs more tuning, confident that with more data will finish work on the Reporter plug-in #IWT14
8:57am September 16th 2014 via Hootsuite
Sartori: Uniformity 94.3%, mean depth 2218x, average on-target mapping 88%; 90% of bases at 500x. #IWT14
8:54am September 16th 2014 via Hootsuite
Sartori: Verification testing begun, 120 total samples, 6 samples on 318. From about 40 samples: 1 amplicon <100x (DNMT3A), 4 <500x #I
8:53am September 16th 2014 via Hootsuite
Sartori: Poorest prior performing amplicons: now significantly improved numbers for CEBPA (9 amplicons) Lowest one still >500x #IWT14
8:51am September 16th 2014 via Hootsuite
