GO:Thus: major limitation for maize is nutrients, not water. N2 and phosphorus. Too little in dev world; too much in 1st wrld #PAGXXIII

11:05am January 14th 2015 via Hootsuite

GO: Map of global consumption 2012 Mueller Nature ref http://t.co/zSmEgU7gZy Overlay with Maize-prod areas, nutrient-limited #PAGXXIII

11:03am January 14th 2015 via Hootsuite

GO:Solving the nitrogen problem - we are heavily dependent. N2 fertilizers causing nitrification, algal blooms #PAGXXIII

11:02am January 14th 2015 via Hootsuite

Last plenary: Giles Oldroyd (John Innes Centre UK) Engineering Nitrogen-Fixing Symbiotic Associations in Cereals #PAGXXIII

11:00am January 14th 2015 via Hootsuite

Over next weeks my updates from #PAGXXIII (both video and posts) will be live on @lifetech's Behind the Bench http://t.co/FUiRKO0eJh

10:41am January 14th 2015 via Hootsuite

RT @moorejh: Great Nature Reviews Genetics paper on data integration by @MarylynRitchie & @sap_PSU http://t.co/atzTMeD8BA #bioinformatic

10:31am January 14th 2015 via Hootsuite

RT @biomickwatson: Some details on the new HiSeq 3000 and 4000 http://t.co/M9a9ojeP6d

9:25am January 14th 2015 via Hootsuite

More at #AGBT15 MT @genetics_blog: 10X Genomics Raises $55.5M. Seems to be a hack on ILMN machine to get long reads. http://t.co/dmPVEdnS5F

8:20am January 14th 2015 via Hootsuite

RT @Farrell__JD: “@klsywd: Some of the vendors are robots this year #PAGXXIII http://t.co/NiKM2PXuL9” @Data2Bio

7:47am January 14th 2015 via Twitter Web Client

RT @mclafave: http://t.co/rUj5CDsSvv now has notes from the #PAGXXIII @PacBio workshop. ~50x PacBio seq gives you a pretty solid assembly.

7:45am January 14th 2015 via Hootsuite

MT @moorejh: ID'ing & amending mislabeled #DNA sequences in #genomics DB's - The Scientist http://t.co/sEY1Fg2TgP http://t.co/XK3ZxVDZMW

7:20am January 14th 2015 via Hootsuite

RT @KAMChapman: Wonderful - sometimes a speech goes even further than even a paper. http://t.co/eQATQl0TPp @npch #Force2015

5:45am January 14th 2015 via Hootsuite

RT @globeandmail: ICYMI: Scientists unleash the power of immunotherapy on stubborn cancers http://t.co/aiWOwgFGJT

4:15am January 14th 2015 via Hootsuite

RT @SMToolsGo: How to Power-Up OS X Spotlight with Flashlight via @lifehacker http://t.co/hSUN1c1kfU

3:05am January 14th 2015 via Hootsuite

RT @lexnederbragt: The PromethION as 'the Borg version of the MinION' http://t.co/W1g0kC0Sk0 Nicely done by @OmicsOmicsBlog #nanopore

2:01am January 14th 2015 via Hootsuite

RT @humanlongevity: Personal Genome Diagnostics, Inc. and Human Longevity, Inc. Sign Cancer Genomics Agreement http://t.co/bNId7DbHDg

1:05am January 14th 2015 via Hootsuite

Woman recreates portraits of her ancestors going back 200 years | i100 http://t.co/SrXU00BEt3

12:20am January 14th 2015 via Hootsuite

RT @cbtadvisors: Look out, @Organovo MT @3dersorg OxSyBio raises £1m 3D printer 4synth tissue http://t.co/FfFLVCEL4z http://t.co/TFgcftOMoH

11:50pm January 13th 2015 via Hootsuite

RT @AstroTerry: #Astrobutch took this early this morning- Mid Atlantic USA with #aurora and #sunrise- wow! http://t.co/e0baMWi2eq

10:40pm January 13th 2015 via Hootsuite

NT:New trials established for TME14. Acknowledges @gatesfoundation #PAGXXIII

8:45pm January 13th 2015 via Hootsuite

NT:But most plants also affected by Cassava Mosaic Disease - now acquired susceptibility. CMD2 appears turned off (!) #PAGXXIII

8:43pm January 13th 2015 via Hootsuite

NT:Concl: siRNA strategy 'appears to hold sig potential for controlling CBSD' Ongoing: virus sequencing, graft challenge PAGXXIII

8:41pm January 13th 2015 via Hootsuite

NT: Overlapping: Mtwapa (Coastal Kenya), to test different context of viruses. 19 lines, very high resistance 18/19 <5% symptoms #PAGXXII

8:39pm January 13th 2015 via Hootsuite

NT:8 mos later with stake-derived plants (not cultured), very similar results. #PAGXXIII

8:38pm January 13th 2015 via Hootsuite

NT:11 lines had <5% any disease symptoms; correlated with high siRNA expression #PAGXXIII

8:36pm January 13th 2015 via Hootsuite

NT: Every plot surrounded by CBSD-infected plots TME204. Every plant scored for loss (1-5). Photos - wt vs transgenics #PAGXXIII

8:35pm January 13th 2015 via Hootsuite

NT: At 14w post-graft; 5/9 were CBSV and UCBSV-resistant, correlated to high expressors. April 2013 25 transgenic lines planted #PAGXXIII

8:33pm January 13th 2015 via Hootsuite

NT:Developed CBSD res TME204; after 425 transgeneic lines (2011-12), 350 screened for low-copy. 52 met low-copy, VBB-free #PAGXXIII

8:32pm January 13th 2015 via Hootsuite

.@JChrisPires Certainly - I view it as a public duty of sorts, although sometimes very difficult to look up with only a name+year! #PAGXXIII

8:28pm January 13th 2015 via Hootsuite in reply to

NT: Cassava brown streak disease (CBSD), transmitted by white flies (like CMD), leaf mottling, brown lesions on storage roots #PAGXXIII

8:27pm January 13th 2015 via Hootsuite

NT:Provided at no charge from the tech developers; a public-service project. Partners w/KALRI (Kenya), Uganda etc. #PAGXXIII

8:26pm January 13th 2015 via Hootsuite

NT:RNAi-mediated resistance; confined field trials; virology; regulatory; infrastructure and regulatory capacity; comm's #PAGXXIII

8:25pm January 13th 2015 via Hootsuite

NT:VIRCA is a product-dev project: generating preferred cassava varieties to small-holder farmers in Africa #PAGXXIII

8:24pm January 13th 2015 via Hootsuite

Nigel Taylor (Donald Danforth Plant Science MO) "The VIRCA Project" (Virus Resistant Cassava for Africa) #PAGXXIII

8:23pm January 13th 2015 via Hootsuite

MW:Q:What about CMD1? A:If anyone has the paper... can't locate that SSR marker... #PAGXXIII

8:20pm January 13th 2015 via Hootsuite

MW:First GWAS in Cassava - w/rapid progress in determining CM2 location. One new small resistance effect gene located on chr14 #PAGXXIII

8:19pm January 13th 2015 via Hootsuite

MW: 11MB region; co-locates with known SSR markers. Work suggests a third locus (CMD3) #PAGXXIII

8:17pm January 13th 2015 via Hootsuite

MW:15y, 13 locations of phenotype data (38.8K observations), scored on CMD severity. Showed GWAS, major QTL on chr8 #PAGXXIII

8:10pm January 13th 2015 via Hootsuite

MW:6128 clones genotyped & phenotyped; 40K SNPs (MAF >0.05). 2 gen of genomic selection progeny #PAGXXIII

8:07pm January 13th 2015 via Hootsuite

MW:Existing improvements are built on a narrow base (CM2) 2014 Virus Res http://t.co/yS8BLCRp4u #PAGXXIII

8:06pm January 13th 2015 via Hootsuite

MW:Part of NextGen Cassava Project http://t.co/VyXDAQPdsz Describes Cassava Mosaic Disease, progress in dev. CMD resistance #PAGXXIII

8:04pm January 13th 2015 via Hootsuite

Marnin Wolfe (Cornell Univ) "Genome-Wide Association Analyses Reveal Key Aspects of Genetic Architecture Cassava in Africa" #PAGXXIII

8:02pm January 13th 2015 via Hootsuite

RP: Want to use genome editing to target ID'd deleterious muts. Again credits the @gatesfoundation for their support. #PAGXXIII

8:01pm January 13th 2015 via Hootsuite

RP:In progress: RNA-Seq transcriptome assemblies from 6 strains to obtain annotations #PAGXXIII

7:55pm January 13th 2015 via Hootsuite

RT @KATAphone: Link to BayesR software coded by Moser. Input is PLINK! http://t.co/IBQU9xnNfj .Thank you @drhansdd !! #PAGXXIII @Farrell__JD

7:51pm January 13th 2015 via Hootsuite

RP:Obj: ID deleterious mutations, then model it. Generate Cassava hapmap, use annotations. Test against inbred NaCRRI pops #PAGXXIII

7:48pm January 13th 2015 via Hootsuite

RP: Clonally propagated - deleterious muts accumulate. A constant mutational pressure. Est 9 deleterious muts in maize/gen #PAGXXIII

7:46pm January 13th 2015 via Hootsuite