AB:Shows data from transgenic plants where the secalin protein ID'd and down-regulated. Tested mixing, data for stickiness #PAGXXIII
2:50pm January 13th 2015 via Hootsuite
AB:Rye translocations in wheat cause stickiness - ID omega secalins (storage protein of rye). Test via RNAi #PAGXXIII
2:45pm January 13th 2015 via Hootsuite
Ann Blechl (USDA-ARS Albany) Using Proteomics in Transgenic Wheat... RNA Interference Targets a Family of Seed Storage Proteins #PAGXXIII
2:43pm January 13th 2015 via Hootsuite
YL:Looked at relationships between 147 ID'd genes; edges show 19 correlated with fiber length #PAGXXIII
2:25pm January 13th 2015 via Hootsuite
YL:In multi-year field trial - 474 GFL genes; 2y used in field trial; each gene had small effect; 11.4% pos effect when up-reg #PAGXXIII
2:20pm January 13th 2015 via Hootsuite
YL: Using forward genetics approach: high thruput, can be done reference-free. ID new gene Gossypium Fiber Length (GFL) #PAGXXIII
2:19pm January 13th 2015 via Hootsuite
YL:500K ESTs; 1K QTLs controlling fiber quality; only 151 related to fiber length. Goal to ID mechanism, improve lengths #PAGXXIII
2:17pm January 13th 2015 via Hootsuite
Yun-Hua Liu (Texas A&M) Molec. Mech of Crop Quality: Cloning and Systems Analysis of 474 Cotton Genes Controlling Fiber Length #PAGXXIII
2:15pm January 13th 2015 via Hootsuite
HZ:Concl: host-pathogen interaction is complex, nuanced, where genetics do play an important role #PAGXXIII
2:11pm January 13th 2015 via Hootsuite
New Post: TaqMan Universal PCR Master Mix - Accept No Substitutes | Behind the Bench http://t.co/msOBN9kfER
2:10pm January 13th 2015 via Hootsuite
HZ:Using LC MS/MS, can ID thousands of proteins between two groups. 2-3K proteins ID'd. Diff expression. Better overlap cp to RNA #PAGXXIII
2:08pm January 13th 2015 via Hootsuite
HZ:Pathway analysis with subtle diff gene exp levels: RIG-I-like receptor signalling in trachea. Also gene-gene interactions #PAGXXIII
2:06pm January 13th 2015 via Hootsuite
HZ:Looking at RNA-Seq data to look at differential expression between tissues; wanted larger systems biology approach #PAGXXIII
2:03pm January 13th 2015 via Hootsuite
HZ: Looked at HTP proteomics and RNA-Seq both host proteins and viral proteins from infected tissues. Fayoumi more resistant #PAGXXIII
1:59pm January 13th 2015 via Hootsuite
HZ: Promising alternative approach is genetic selection for AIV resistance. Two diverse inbred chicken lines Legorn Fayoumi #PAGXXIII
1:58pm January 13th 2015 via Hootsuite
HZ: AIV is type A. HA attaches, NA releases virions from cells. 2y ago H7N9 from China: dozens of fatalities. Major econ loss #PAGXXIII
1:54pm January 13th 2015 via Hootsuite
HZ: Influenza - Avian bird flu is a human health concern. Highly pathogenic strains arise sporadically in CA, WA #PAGXXIII
1:53pm January 13th 2015 via Hootsuite
Huaijun Zhou (UC Davis) Systems Biology Analysis of Mechanism of Host Response to Avian Influenza Virus in Two Chicken Lines #PAGXXIII
1:50pm January 13th 2015 via Hootsuite
RT @KristaTernus: .@mike_schatz is describing his Oxford Nanopore MinION work. Learn more in his preprint http://t.co/1EhkUGCb0O #PAGXXIII
1:46pm January 13th 2015 via Hootsuite
SH:Methylation patterns in both gene-body and intergenic regions; evidence for tissue-spec gene exp #PAGXIII
1:45pm January 13th 2015 via Hootsuite
SH:Looking at single-cell epigenetic modification, BS-Seq w/heat stress. DMR's changes in both single- and multi-cells #PAGXIII
SH: Describing novel miRNAs called gma-miR171 targeting GRAS1, nodulation phenotype #PAGXIII
1:41pm January 13th 2015 via Hootsuite
Shakhawat Hossain (U Missouri) Soybean Root Hair, a Single Cell Model to Study Plant-Microbe Interactions, Epigenetic Regulation #PAGXXIII
1:40pm January 13th 2015 via Hootsuite
MG: Not a problem of more markers or WGS - just a problem of many markers of very small effect #PAGXXIII
12:15pm January 13th 2015 via Hootsuite
RT @pop_gen_JED: MG extremes of traits cause other health and fitness issues (eg dwarfs and giants). Small effects receive less selection p…
12:07pm January 13th 2015 via Twitter Web Client
RT @pop_gen_JED: MG mutations of large effect get strongly selected against. As a group explain small portion of total variance. #PAGXXIII
MG: Est proportion of SNPs using this method: across four traits (food conversion, weight etc.) mean 19 SNPs acct for 1% #PAGXXIII
11:52am January 13th 2015 via Hootsuite
MG: A better cattle model: BayesR fits a mix of normals (2012 Erbe et al J Dairy Sci http://t.co/GhHRIVEkX7 ) #PAGXXIII
11:50am January 13th 2015 via Hootsuite
MG: 2005 Johnson & Barton paper: http://t.co/h9ECYQbxjl Showing GWAS for cattle weight - long-distance LD. Large # effects #PAGXXIII
11:48am January 13th 2015 via Hootsuite
MG: Quotes Johnson & Barton 2005 "observing remarkably robust patterns... yet seeing no compelling explanation for them" #PAGXXIII
11:45am January 13th 2015 via Hootsuite
MG: By architecture: underlying # of loci, size of effects, allele freq, and how they come about as a result of drift, selection #PAGXXIII
Mike Goddard (Univ Melbourne, Australia) "The Evolution and Genetic Architecture of Complex Traits" #PAGXXIII
11:41am January 13th 2015 via Hootsuite
XC:Q:URT1 prefers truncated; does URT1 prefer erroneously-cleaved DICER prods? A:Yes, likely #PAGXXIII
11:40am January 13th 2015 via Hootsuite
XC:Q:Why Uridylation before degradation? A:Perh. to mark for degradation, some nucleases may prefer them #PAGXXIII
XC: miRNA, siRNA, piRNA, pre-miRNA can all be uridylated then degraded as well #PAGXXIII
11:38am January 13th 2015 via Hootsuite
XC: Ref 2004 Science paper http://t.co/n7j88dBbAc about addition of U to mRNA after miRNA-mediated cleavage. Now 'rediscovered' #PAGXXIII
11:37am January 13th 2015 via Hootsuite
XC: In animals, piRNA methyl by HEN1; illus. different modes of action bet plants & animals 2010 Silence http://t.co/r3M71GMUv8 #PAGXXII
11:33am January 13th 2015 via Hootsuite
XC: Idea - target RNA induces miRNA deg.; synthetic target (uncleavable), supports this 2012 Plant Cell http://t.co/ufxAD57BaL #PAGXXIII
11:30am January 13th 2015 via Hootsuite
XC: Function of 3' tails: 2012 Ann Rev Cell Dev Biol http://t.co/HhIf0SH7x3 #PAGXXIII
11:28am January 13th 2015 via Hootsuite
XC: Full-length miRNA; trunc.by SDN, tailed by URT1/HESO1. Unk enz degrades tails. TBD whether AGO1 binding affects degradation #PAGXXIII
11:24am January 13th 2015 via Hootsuite
XC: hen1 muts shown; other 9 nucleotidyl transferases w/identical patterns of tailing; A vs U pref of URT1 cp to HESO1 #PAGXXIII
11:22am January 13th 2015 via Hootsuite
XC: What deposits tail? Zhao 2012 Curr Biol http://t.co/RlZiMYRGHv describes hen1 suppressor (heso1) reducing tailing #PAGXXIII
11:19am January 13th 2015 via Hootsuite
XC: Cp full-length, truncated, truncated & tailed, tailed-only species via NGS. SDN proteins resp for miRNA 3' truncation #PAGXXIII
11:17am January 13th 2015 via Hootsuite
XC: Work from 2008 Science http://t.co/8o4dtStFJ5 of Small Degrading Nuclease genes SDN1/2 genes #PAGXXIII
11:15am January 13th 2015 via Hootsuite
XC: Conservation of HEN1 mechanism in both plants / animals. Uridylation protection 2014 Curr Biol http://t.co/2fUZ6A13V5 #PAGXXIII
11:14am January 13th 2015 via Hootsuite
XC: Steady state between biogenesis / degradation: little known about the latter. 2'-O-methylation key step of biogenesis #PAGXXIII
11:12am January 13th 2015 via Hootsuite
XC: piRNA, siRNA, miRNA impact 'nearly all biological processes' - patterning, reprod, development #PAGXXIII
11:08am January 13th 2015 via Hootsuite
RT @PhilippBayer: 2988 registered participants now! #PAGXXIII
11:07am January 13th 2015 via Hootsuite
Xuemi Chen (UC Riverside USA) "A tale of tails: Plant microRNAs: biogenesis, degradation, and modes of action" #PAGXXIII
11:06am January 13th 2015 via Hootsuite
RT @kmkubo: What College Professors Can Learn From Alan Alda — TIME http://t.co/rFia6kTnet via @AldaCenter
9:10am January 13th 2015 via Hootsuite
