ZW: ID'd thousands of events, but overlap not high. Det. 163 cancer-spec alternative splicings, about 5x than expected by chance #NHLBI
11:50am March 19th 2015 via Hootsuite
ZW: Using @TCGAupdates data, ID'ing splicing changes indep of tissue type. First focused on Br, Liver, Lung #NHLBI
11:45am March 19th 2015 via Hootsuite
ZW: In NSCLC pts - looking at levels of RBM4, Bcl-xL, Meier-Kaplan of survival shifts with differential RBM4 expression #NHLBI
11:41am March 19th 2015 via Hootsuite
ZW: RBM4 antagonizes the oncogenic splicing factor SRSF1 to inhibit mTOR activation. Data from cell lines #NHLBI
11:39am March 19th 2015 via Hootsuite
ZW:By inhibition of bcl-xL form, RBM4 induces apoptosis. Can rescue phenotype by reinducing bcl-xL #NHLBI
11:38am March 19th 2015 via Hootsuite
ZW: Looking at bcl-x splicing, regulated by RBM4, by expts that mutated the RBM4 binding site (reduced bcl) then restored #NHLBI
11:34am March 19th 2015 via Hootsuite
ZW: In NSCLC mouse model xenografts - showed RBM4 inhibited tumor growth #NHLBI
11:32am March 19th 2015 via Hootsuite
ZW: This RBM4 inhibits cancer cell growth and migration; data from many different cell line types. #NHLBI
11:31am March 19th 2015 via Hootsuite
ZW: Trans control, RNA processing, cell-migration, mRNA metabolism, NF-kB cascade #NHLBI
11:30am March 19th 2015 via Hootsuite
ZW: Of these, six different types of alt splicing (exon skip, tandem UTR etc); found RBM4 controls five types of cancer-related genes #NHLBI
11:29am March 19th 2015 via Hootsuite
ZW: A family of ~20 RBM trans-factors; in a cell-line w/low RBM4 endogenous, looked at role in alt splicing. ID'd 200 events #NHLBI
11:28am March 19th 2015 via Hootsuite
ZW: Focused on one: RBM4 2014 Cancer Cell ref: http://t.co/m52t1IYWXw Acts as a general splicing inhibitor. In-vitro data #NHLBI
11:27am March 19th 2015 via Hootsuite
ZW: These factrors (starred in his diagram) some 10 are trans-factors involved with cancer, tumor progression #NHLBI
11:25am March 19th 2015 via Hootsuite
ZW: 'An overlapping network of trans-factors recognize ISSs and ISEs'. Used HeLa extracts, sites are AC-rich #NHLBI
ZW: Confirmed hnRNP L and Y-box binding proteins in 2/4 bands. 2012 ref: http://t.co/FCfGOa0YAj /KxBH1 Showed trans-factor network #NHLBI
11:23am March 19th 2015 via Hootsuite
ZW: Trans-factors that bind: use affinity purification of ISS 7 groups of 7-mers 'cores'. Via Mass-spec, ID'd 4 protein bands #NHLBI
11:21am March 19th 2015 via Hootsuite
ZW: Looking at enhancers (SE), used GFP insert; ID'd 117 ISE 10-mers, 109 unique. All ISE's function as ESS when in exons #NHLBI
11:18am March 19th 2015 via Hootsuite
ZW:Method: FAS-ISS 2013 Ref: http://t.co/oBNBS12g92 Screened 114 ISS 10-mers ID'd, tested function. Only about 15bp away from U1 #NHLBI
11:17am March 19th 2015 via Hootsuite
ZW: Looks at both trans-factors (snRNPs on U1/2) and cis-factors (Exon SE and SS, Intron SE and SS) SS=splicing suppressor E=enhancer #NHLBI
11:15am March 19th 2015 via Hootsuite
ZW: Splice sites only have about 1/2 the information needed. 2001 Lim PNAS http://t.co/dVTeQ2PFt3 Rest is cis-elements #NHLBI
11:13am March 19th 2015 via Hootsuite
ZW: Both exons and introns have enhancers and suppressors of splicing in cis #NHLBI
11:12am March 19th 2015 via Hootsuite
ZW: Working out code, discuss mis-regulated splicing code in disease, and then (future) the promise to reprogram the code. #NHLBI
11:11am March 19th 2015 via Hootsuite
ZW: Shows UCSC track of scored splice sites, shows many pseudo-exons that never get spliced in. No imposters get spliced in, ever #NHLBI
11:10am March 19th 2015 via Hootsuite
ZW: Pre-mRNA splicing controlled at 3' and 5' sites; higher scores match to consensus seq. snRNPs have U1/U2; U4-U6 forms spliceosome #NHLBI
11:09am March 19th 2015 via Hootsuite
ZW: More than 90% of genes undergo alt splicing; different isoforms have different (and often opposite) functions. #NHLBI
11:07am March 19th 2015 via Hootsuite
ZW: Jun Zhu introduces Dr. Wang speaking @nih_nhlbi - Zefeng will soon be moving to the Max Planck Inst. presence in China.
11:06am March 19th 2015 via Hootsuite
Zefeng Wang (UNC) here at NIH/NHLBI: "Towards a Splicing Code-regulation and Mis-regulation of Alternative Splicing in Cancers"
11:04am March 19th 2015 via Hootsuite
What Your Tweets Say About You - The New Yorker http://t.co/y7OVg6ffbs HT @drbachinsky
10:55am March 19th 2015 via Hootsuite
RT @NoahFierer: New blog post: Pet peeves in 16S rRNA-based microbiome studies http://t.co/N33Jh60QaF
9:25am March 19th 2015 via Hootsuite
8,000 Years Ago, 17 Women Reproduced for Every One Man - Pacific Standard http://t.co/RT2Suqno3i
8:45am March 19th 2015 via Hootsuite
New Post: #AGBT15 Ion Torrent Technologies Update with Alan Williams | Behind the Bench http://t.co/bjBqfeDLQo
7:15am March 19th 2015 via Hootsuite
Neuroscience Virtual Event 18-19 Mar 2015 | Labroots http://t.co/lDMf7Spp5M
6:40am March 19th 2015 via Hootsuite
RT @IBMWatson: What does your writing style say? Find out with Personality Insights http://t.co/t4jlhqIZVa http://t.co/dXZV3lgPhV
5:25am March 19th 2015 via Hootsuite
A new study finds there are too many scientific studies - Vox http://t.co/ZIV5QvgPuu
4:40am March 19th 2015 via Hootsuite
RT @BuzzFeedScience: The history of Europe lies in British people’s DNA http://t.co/QoTYb2zld9 http://t.co/UQbiHl8hPz
10:25pm March 18th 2015 via Hootsuite
RT @NatureNews: British Isles mapped out by genetic ancestry http://t.co/IseiL8IgQ4 http://t.co/VAFzd1Wzeq
9:20pm March 18th 2015 via Hootsuite
While listening to @mendelspod 'This month in biotech' http://t.co/XFLm8wsLxK is this gem from @genomenathan http://t.co/5OMokBUTvH
8:51pm March 18th 2015 via Hootsuite
RT @fluidigm: We make microfluidic and mass cytometry systems for #singlecell RNA, DNA, and protein. Our events aren't bad either. http://t…
7:54pm March 18th 2015 via Twitter Web Client
Pearson Under Fire for Monitoring Students' Twitter Posts - http://t.co/dDcpBPkdXU http://t.co/NzFHKIaCHp
7:15pm March 18th 2015 via Hootsuite
RT @moorejh: Great post from @EcoDevoEvo on 'Simulating #complexity and predicting the future' http://t.co/zY53eCEbNx #precisionmedicine
6:50pm March 18th 2015 via Hootsuite
RT @MishaAngrist: "The role of regulatory variation in complex traits and disease" @leonidkruglyak http://t.co/BAZl1bX4sw
5:50pm March 18th 2015 via Hootsuite
How far a paycheck goes in 191 U.S. cities - The Washington Post http://t.co/ARzwl1rlnl
4:00pm March 18th 2015 via Hootsuite
Functional annotation of native enhancers with a Cas9-histone demethylase fusion | Nature Methods http://t.co/D83ImGuUtG HT @billbarendse
3:40pm March 18th 2015 via Hootsuite
RT @billbarendse: RT @NatureNews “We are going down the path of having a myopia epidemic” Why? http://t.co/Cx6JWOvFyq http://t.co/YHUiluqOFn
2:40pm March 18th 2015 via Hootsuite
RT @infoecho: Like to participate next revolution using long reads @PacBio with your bioinformatics/algorithmic insights? DM me.
1:05pm March 18th 2015 via Hootsuite
Germline Genome Editing Debate | GenomeWeb http://t.co/Wc4OxWorKT
12:20pm March 18th 2015 via Hootsuite
RT @edyong209: This fish uses a tongue made of water to swallow its food while it walks on land. http://t.co/z8WGWCMqmT
11:31am March 18th 2015 via Hootsuite
Scientists’ New Goal: Growing Old Without Disease - WSJ http://t.co/w5u3IeiGsP
10:40am March 18th 2015 via Hootsuite
Great stuff. Genomes, Hype, and a Realistic Pathway to Personalized Medicine - YouTube http://t.co/SG9SnbppNG #precisionmedicine
9:15am March 18th 2015 via Hootsuite
A Sucker Is Optimized Every Minute - http://t.co/dDcpBPkdXU http://t.co/PZQLH7yC2E
8:15am March 18th 2015 via Hootsuite
