FD: DNA Multiplex eval - moving from SGMplus, eval DNA17 and DNA20+ kits for repeatability, send, mixed & partial, analysis #HIDS15
7:36am March 11th 2015 via Twitter Web Client
FD: Outside interface is Police Scotland interaction, and UK National DNA database #HIDS15
FD: No R&D 'unfortunately at this time'. Showed flowchart of their model - scene examination, forensic services, then database #HIDS15
7:35am March 11th 2015 via Twitter Web Client
FD: Scene examination, physical sciences, biology (incl. DNA), business support. Reduced costs and better efficiency #HIDS15
FD: '13 move to new facility. Modernization took 4 different labs and combined them. Function: crime scene to court w/best practice #HIDS15
FD: 8 year changes in Scotland: '07 Police Services Authority, '10-'13 Forensic Modernization, '13 single Police authority #HIDS15
Fiona Douglas, Forensic Services (SPA), Glasgow, Scotland. Building a state of the art forensic service #HIDS15
CH: Contamination rate: cut in half to 6%. Electronic reporting. # of FTE's now is 6 for all sample handling #HIDS15
CH:FTE requirements: 12FTE to start, Jun '14: only 6. Now in Nov '14: only 2. Staff valuable for reporting unit #HIDS15
7:34am March 11th 2015 via Twitter Web Client
CH: Chart of turn-around time. Apr '14:10-12d; Jun '14:4d, Nov '14: only 2d via small adjustments in staffing, processing #HIDS15
CH: They have the capacity for 70K/yr. Remote monitored as well (for @appliedbio 7500 and AB3500 too) #HIDS15
CH: After lysis - another robot for extraction, then another for STR setup, then PCR, then robot for CE setup. 35K/year thrupt #HIDS15
CH:On-robot centrifugation, lifting the inner tube, etc. Protocol GANTT-like chart shown with overlapping protocols. #HIDS15
CH: RAT tube association to electronic file - can be racked and scanned automatically. Recapping, lysis, all automated #HIDS15
CH: Sample receipt in sealed individual barcoded envelope; electronic information automatically uploaded to their LIMS #HIDS15
CH:A field Robot Acceptable Tube (RAT), no manual handling of samples. #HIDS15
CH: Autolysis tubes - tube-in-tube device, lift-and-lock separates tube from lysate, removal of debris via inner tube. Barcoded #HIDS15
CH: Hamilton auto-lysis - manual takes 3 FTE's 4h hands-on for 96. Automated: one FTE 30m hands-on for 96. Takes 3h #HIDS15
CH: Robot ready-tubes, but lysis still labor-intensive. Buffer, incubate, remove substrate for spinning, centrifuge, onto robot #HIDS15
7:33am March 11th 2015 via Twitter Web Client
CH: Before '11: all items sent, collect samples via swab, tape-lift, time-consuming. Initiative for field sub-sampling #HIDS15
CH:'12 AutoMate Express, '13 new CE robot; 2014 automated lysis and lab refurbished #HIDS15
CH:'09 all manual; then: 6 Tecans for quant, STR. '10, '11: PrepFiler (dev of lysis suitable for automation) and LIMS #HIDS15
CH: Australia is 23M; NSW is 7.5M (32% of country); NSW is 'almost double the area of Spain' Focus on automation #HIDS15
Catherine Hitchcock (NSW Health Pathology, Australia) End-to-end automation driving efficiencies in high throughput DNA analysis #HIDS15
AA: Concludes w/Globalfiler equivalent overall performance; beneficial to improve discrimination power #HIDS15
AA: Showed samples of low and high degradation with partial NGM Select and Globalfiler profiles #HIDS15
AA: Series of 3ng total DNA at 10:1 mix ratios. Then chart of mixed samples from GHEB / GEDNAP proficiency samples #HIDS15
AA: Globalfiler profile of ref. saliva swab shown; showed 22 PCR-neg controls (75-450bp); chart of 5 colors & RFU stats #HIDS15
7:32am March 11th 2015 via Twitter Web Client
AA: Slide on extraction, quant via Quantifier Duo, AB3500 detection. #HIDS15
AA:IDNADEX - published in FSI Genetics http://t.co/cEmKSEBH0F Will present validation parameters (sens., DNA mixture data) #HIDS15
AA: Validating a single 21 STR typing system based on 6-dye CE detection. Large chart of ESS STR, CODIS, other STR, Spanish db #HIDS15
Antonio Alonso (National Toxicological Institute for Forensic Sciences, Madrid, Spain) Improving DNA data exchange #HIDS15
SV: At >33pg/uL, DI >4, expect partial profile from GlobalFiler and 1ng input #HIDS15
SV: Tested 22 samples: 1ng GF, 0.5ng GF or NGM-SE 0.5ng. When DI >4, partial or no profile expected at 3-33 pg/uL, use Mini-STR #HIDS15
SV: % of markers called above allele length charted against marker type, binned against DI index, at both 0.5ng and 45pg-500pg #HIDS15
7:31am March 11th 2015 via Twitter Web Client
SV: Deduced DI index range: <1.5 no degradation; 1.5-4 mild; 4-10 degraded; >10 severely degraded #HIDS15
SV: W/GlobalFiler, showed a reasonable linear relationship (r^2=0.8) of value p to DI plot using regression model #HIDS15
SV: Showed peak heights as a fn of DI value; Used Morling's '12 ref http://t.co/32Ood7PIhx of degradation coefficient #HIDS15
SV: Compared QF Trio to QF Duo - 181 samples, showed pie chart w/quant data. Info on Quantifier Trio https://t.co/bVsJTtkn0T #HIDS15
SV: One Small Autosomal (80bp), Large Autosomal (214bp) and Y-chr (75bp). Index = [SA]/[LA]. DI =1 little degradation #HIDS15
SV: Cost, time and sample-consuming: many times degraded & precious. Quantifier Trio: both conc and quality #HIDS15
SV:Illustrates the issues of DNA degradation; typically using mini STRs, combining different STR kits, and/or increase DNA #HIDS15
7:30am March 11th 2015 via Twitter Web Client
Stefano Vernarecci Rome Police: "Quantifiler Trio kit and forensic samples management: a matter of degradation" #HIDS15
PS:Q:European exclusive? A:No, it's international and free to join. #HIDS15
PS: Also networking, ethical & legal & societal dimensions of forensic genetics #HIDS15
PS: Has organized EuroForGen >180 laboratories http://t.co/tX3XrxWG1h A 'virtual institute' with training, original research #HIDS15
PS: Pie chart of US funding (NIJ) Total 2008-2014 $30M, $6.8M in '14 #HIDS15
PS: Article about EU's leadership http://t.co/5e2ERdYvpn Yet contrasted with % of paper decline in 2 forensics journals #HID15
7:29am March 11th 2015 via Twitter Web Client
PS: The evidence challenge: what cell-type did the DNA originate from? ("The CSI effect") DNA transfer, persistence, ass'ns #HIDS15
PS: mRNA is a novel tool for forensics - what was source of the sample? (While DNA gives 'who'.) It can help reconstruct event #HIDS15
