RT @kirkenglehardt: Technology meets musical instruments - the ten coolest right here http://t.co/OrONb5SCSO @GeorgiaTech
9:15am March 4th 2015 via Hootsuite
RT @mashable: How do you warn someone 10,000 years into the future? http://t.co/y7pLfHyhPF via @TheAtlantic
8:31am March 4th 2015 via Hootsuite
RT @macworld: Have we reached peak smartphone? If so, what's next? http://t.co/51WAGwL9LJ http://t.co/XxDXfcAuxj
7:20am March 4th 2015 via Hootsuite
.@milospm1206 (Check out #HIDS15 for my live-tweeting notes FWIW on this forensics conference.)
6:58am March 4th 2015 via Hootsuite in reply to
.@milospm1206 Earlier today I interviewed Tom Parsons, incredible story. http://t.co/UJ9MhxFiRZ 35K victims from Bosnia, ID'ing 70% of them.
6:57am March 4th 2015 via Hootsuite in reply to
.@milospm1206 I never thought I'd ever be in front of a camera. Oh well you never know where an interest will lead.
6:56am March 4th 2015 via Hootsuite in reply to
RT @Forbes: “If the phone companies had made SMS free, it would have been difficult to have been successful:" http://t.co/VXd6xEbHyM
6:50am March 4th 2015 via Hootsuite
.@SamanthaZeitlin @westr @ctitusbrown It's spelled 'hydatidiform' and here's the Genome Res paper: http://t.co/w9IAd3UcOW
5:55am March 4th 2015 via Hootsuite in reply to
RT @PiescheM: Cutting-edge study of potential #HIV 'cure' wins #FDA nod http://t.co/285TcrsBuk
5:50am March 4th 2015 via Hootsuite
.@shanemuk Was it this "Every life has value" podcast? http://t.co/l3ygyy50hz
5:32am March 4th 2015 via Hootsuite in reply to
.@westr @SamanthaZeitlin @ctitusbrown Alas no messages (yet) of 'unsequencing the unsequence-able'. Complex regions - http://t.co/7hV1EN0m8p
4:47am March 4th 2015 via Hootsuite
.@westr @SamanthaZeitlin @ctitusbrown While PacBio covered long-reads at AGBT, focus was on their hydatidiform mole human WGS de-novo assy
4:41am March 4th 2015 via Hootsuite in reply to
RT @BBCWorld: Looted by the Nazis and missing for 50 years, paintings are on public display http://t.co/leunGJ7keS http://t.co/NQAq75gB0j
4:05am March 4th 2015 via Hootsuite
RT @GenomeWeb: New biomedical group at @CanonUSA sets sights on molecular testing platform http://t.co/kPN5yQCpDN
11:30pm March 3rd 2015 via Hootsuite
RT @ctitusbrown: #G10k Stephen O'Brien: tl;dr? "let's sequence everything we can catch!"
9:40pm March 3rd 2015 via Hootsuite
Mental Health May Depend on Creatures in the Gut - Scientific American http://t.co/1FeJde4NTg
8:30pm March 3rd 2015 via Hootsuite
#AGBT15: Video and Poster: CTC's and Low Frequency Variant Detection http://t.co/eFV7fSdBbl
7:05pm March 3rd 2015 via Hootsuite
AW: <$1k/run for PII; 24 samples/chip; up to 6 whole transcriptomes/chip (dep on depth req'd) #AGBT15
6:41pm March 3rd 2015 via Hootsuite
AW: First product for PII will be AmpliSeq Transcriptome. Product info http://t.co/Up1Kg5ISTC #AGBT15
6:40pm March 3rd 2015 via Hootsuite
AW: Sample was human genomic DNA, fragment library. EA first half, full launch second half of 2015 #AGBT15
AW: 5GB/s data: PII run, >30G total bases, >300M aligned; 115 base mode readlength. Target is 200-300M read range. #AGBT15
AW: 3um wells, 1.3um PI w/165M wells, 0.5um PII w/660M wells. Challenges - chemistry, electrical. 'We have a revised chip now' #AGBT15
AW: OneTouch launched this month, Ion Chef soon. (One measured on Ion AmpliSeq Exome >50K variants) #AGBT15
AW:Hi-Q fully launched on both PGM and Proton, OneTouch and Ion Chef. Showed 75% and 81% reduction in indel FP's #AGBT15
6:39pm March 3rd 2015 via Hootsuite
First: Alan Williams of Thermo Fisher, 'Continuing the evolution of Ion Torrent NGS Technology' #AGBT15
Warning: live-tweeting from the Thermo Fisher workshop from http://t.co/DkrG24evul #AGBT15
DG:Work is ongoing but read-depth does indicate gene amplification (i.e. determine copy number via a custom AmpliSeq panel) #AGBT15
6:38pm March 3rd 2015 via Twitter Web Client
DG:Designing a CNV research panel: @iontorrent 'white glove' team assisted, data of 10ng, 5ng and 1ng identical #AGBT15
DG:Found depth of >10,000x; 1ng or 10ng they got the same data; was able to drive limit of detection down to 1% #AGBT15
DG:More recently, designed a custom panel to push limit of sensitivity. Used AmpliSeq Designer (online) w/shorter amplicons #AGBT15
6:37pm March 3rd 2015 via Twitter Web Client
DG: Using Cancer Hotspot Panel v.2, of >500 samples, have been able to detect down to 3% #AGBT15
DG: SNV 98% detection for 5% minor variant frequency. Found her2 amplification that they could pick up. #AGBT15
DG:Reviews cfDNA - downstream of apoptosis, necrosis, active secretion. Why popular: accessibilty, could yield all subclone info #AGBT15
David Guttery (U Leicester) Targeted seq of cell-free DNA using the Ion AmpliSeq™ Cancer Hotspot & custom DNA research panels #AGBT15
MW: Acknowledges the responsiveness of @iontorrent, Hamilton at MDA, Sklar at Yale, Iafrete at MassGen #AGBT15
MW:Outlines next steps in preparation of submission of data to FDA as part of the IND, then NY State. Over 2y effort. #AGBT15
6:36pm March 3rd 2015 via Twitter Web Client
MW:'We were surprised at the reproducibility across sites and samples'. 25 SNVs, 10 indels, 10 large indels, 10 CNV, 10 fusions #AGBT15
MW: 3 HapMaps (NA12878, GM18507, GM18526) no discordance at all. Cell lines similarly clean. Of 44 test samples - 96.7% sens #AGBT15
MW: Decided to wait until Ion Reporter v.4.4 for 44 test samples; along w/10 cell lines & 3 HapMap samples #AGBT15
MW:Has 14d turnaround, MD Anderson will do extraction. Central data analysis via Ion Reporter v4.4. #AGBT15
MW: Then harmonized to one SOP, found robustness of assay, locked SOP. Not finished yet but 'everything is looking good' #AGBT15
MW: Required input material, performance metrics, then went with plan to the FDA. Used draft SOPs independently across sites #AGBT15
MW: Mentions the four labs chosen to run the assays - Yale, MD Anderson, Mass Gen, NCI #AGBT15
MW: Information about Oncomine Research Panel here https://t.co/T3t9UofyAt #AGBT15
MW:Another consideration was batch size. Learned from @servingscinece that the Oncomine panel was in development #AGBT15
6:35pm March 3rd 2015 via Twitter Web Client
MW:Evaluation was via RFI; collected a lot of info. & feedback. Selected PGM due to low input req., also speed of turnaround #AGBT15
MW:Reviews eligibility guidelines, want to go for a broad spectrum of tumor types. #AGBT15
MW: ~2,400 sites, incl. comm. hospitals; 1 umbrella IND w/central IRB, single db; will ID mut's, amplifications & translocations #AGBT15
MW: Discussion with FDA and the CDER extensively, used as an investigational device. Molecular Analysis for Therapy Choice #AGBT15
MW:'First of all, I want to say that @servingscience has been a great partner'. #AGBT15
