SR: Took each of the six, mutated back to wild-type, ID'd the second minigene that lost activity. Kinesin family member 2C gene. #NIH
4:43pm April 29th 2015 via Hootsuite
SR: Pt.3: 71 non-syn mut's; made 12 minigenes (6 apiece); only tandem minigene 1 responded. Which of the six in minigene 1? #NIH
4:42pm April 29th 2015 via Hootsuite
SR: Express minigene protein in-vitro, and transfect translated protein directly into antigen presenting cell (!) #NIH
4:41pm April 29th 2015 via Hootsuite
SR: Go back to DNA level - take 25-mer aa's, construct strings of 'minigenes' together (six times); clone & express #NIH
4:40pm April 29th 2015 via Hootsuite
SR: Tandem minigene approach - bind to all of the pt's MHC molecules, simultaneous evaluation, no prediction needed #NIH
SR: Pt2 - GAS7 and HAUS3 proteins were immunogenic. The problem: 100's of Class I/II MHC molecules immunogenic. #NIH
4:39pm April 29th 2015 via Hootsuite
.@sesiegler I'm on it! Thanks for listening.
4:38pm April 29th 2015 via Hootsuite in reply to sesiegler
SR: #2 and #16 (by prediction) bound strongly via TIL co-culture assay. PLEKHM2 and PP1R3B mutants in that individual #NIH
4:38pm April 29th 2015 via Hootsuite
SR: Showed list of all 21-mers w/modified aa's. Pt was HLA-A1, listed candidates, took top 9-mers, synthesized & tested TILs #NIH
4:37pm April 29th 2015 via Hootsuite
SR: Synthesized predicted, and look for in-vitro recognition. One pt: TIL 2369, WES ID'd 595 nsSNVs. Large liver deposit, full remission#NIH
4:36pm April 29th 2015 via Hootsuite
SR: Can ID the 10-11 normal aa on either side of the mutated aa's. Thus that 21aa sequence has all possible 11aa MHC peptides in it #NIH
4:34pm April 29th 2015 via Hootsuite
SR: Thus only a 'small percentage of mutations' are antigenic. ID of which mutation - WES '13 Nature Med ref http://t.co/hZIad0kLMn #NIH
SR: Sequence of their pts - average of 220 ns mutations. For a cancer antigen (Ag): 9-11aa peptide; must fit onto MHC groove #NIH
4:32pm April 29th 2015 via Hootsuite
SR: A puzzle: why is melanoma so immunogenic? Points to a figure from this '13 Nature ref http://t.co/cWPA84e84u melanoma on far right #NIH
4:31pm April 29th 2015 via Hootsuite
SR: K-M curve of complete regression - out to 10y. 'can mediate complete, durable' response. #NIH
4:30pm April 29th 2015 via Hootsuite
SR: No relationship betw. the bulk of the tumor & the response. Graphic, huge melanomas shown, amazing reduction, 5y compl. regression #
4:28pm April 29th 2015 via Hootsuite
SR: 22% of the 93 (20) complete and durable response over many years. Xray of liver: 34d visible decline of lung tumor. #NIH
4:27pm April 29th 2015 via Hootsuite
SR: Lympho-depletion - around 8d process, at maximum depletion transfer is performed. Long followup: 93 cases, 9.8y mean 56% obj. resp #NIH
4:26pm April 29th 2015 via Hootsuite
SR:An also alter the host environment for the transferred cells. Shows slide of adoptive transfer of Tumor Infiltrating Lymphocytes #NIH
4:25pm April 29th 2015 via Hootsuite
SR: Adv of cell xfer therapy: 10^11 cells grown in culture w/high avidity; can activate ex-vivo. ID exact cell sub-populations. #NIH
4:24pm April 29th 2015 via Hootsuite
SR:3rd approach: passively xfer activated immune cells w/anti-tumor activity. (Adoptive immunotherapy) #NIH
4:23pm April 29th 2015 via Hootsuite
SR: Why immunotherapy: non-spec stim (IL-2) or inhibit CTLA4 (10% melanoma), PD-1 (30% melanoma). 2nd form: active immunization: vaccine#NIH
4:22pm April 29th 2015 via Hootsuite
SR: ~1.2M cases of invasive cancer; 1/3 will be affected; 1/6 will die of it;Chemo + radiation will cure ~50%. Metastatic: poor chances #NIH
4:20pm April 29th 2015 via Hootsuite
During the introduction - after 40+ y as head of an NCI division, SR described as a 'transformational leader' at the #NIH
4:19pm April 29th 2015 via Hootsuite
At the #NIH, Steve Rosenberg NCI "Cancer Immunotherapy by Targeting Unique Cancer Mutations" SR (I missed his #AACR15 talk, a 2nd chance!)
4:18pm April 29th 2015 via Hootsuite
The next generation of medical tools may be home-brewed — Medium http://t.co/Uji9wt9IVR
3:30pm April 29th 2015 via Hootsuite
#AACR15 Post Roundup | Behind the Bench http://t.co/B5uyM8XYN2
The Untold Story of Silk Road | WIRED http://t.co/GQ8I4SbOmZ Great piece.
2:20pm April 29th 2015 via Hootsuite
Only 250K Pieces. This Guy’s Giant Lego Collection Proves Everything Is Awesome | Houzz http://t.co/OPoDeRZ9ho
1:30pm April 29th 2015 via Hootsuite
What Tyson’s pledge to stop using human antibiotics in chicken means for the future of superbugs - Washington Post http://t.co/OwPJPJJIXG
12:45pm April 29th 2015 via Hootsuite
All scientific posters from @iontorrent and @appliedbio presented at #AACR15 now available http://t.co/MJTnlgC44n
11:25am April 29th 2015 via Hootsuite
Apple's ResearchKit - The Real Impact on Clinical Trials | Merrilyn Datta | Bioscience Tech http://t.co/kiX6Ghso8o
10:25am April 29th 2015 via Hootsuite
RT @DailyNewsGW: Scientist Find Cas9 Enzyme That Targets HCV in Human Cells. http://t.co/YPjLKkdYGc
9:25am April 29th 2015 via Hootsuite
What you really need to know about Baltimore, from a reporter who’s lived there for over 30 years - Washington Post http://t.co/XAPKinNORL
8:45am April 29th 2015 via Hootsuite
RT @DanKoboldt: “The two most important days in your life are the day you are born and the day you find out why.” -Mark Twain
7:00am April 29th 2015 via Hootsuite in reply to DanKoboldt
RT @_bioengineer: Photovoltaic retinal implant could restore functional sight - Read more: http://t.co/zNX9tvspcr http://t.co/HyP2NpHatI
6:05am April 29th 2015 via Hootsuite in reply to _bioengineer
RT @MicrobiomDigest: Cp of the gut microbiota of obese and lean people in France and Saudi Arabia (12 obese French!) http://t.co/0dNxpxegj7
5:45am April 29th 2015 via Hootsuite in reply to MicrobiomDigest
RT @Helena_LB: The effect of music performance on the transcriptome of professional musicians (@SciReports) http://t.co/a9iwW2ixfA
4:20am April 29th 2015 via Hootsuite
Baltimore Riots: Social Media and the Crisis on My Doorstep - WSJ ($) http://t.co/i2FpdpPn32 by @mims
11:45pm April 28th 2015 via Hootsuite
RT @TheEconomist: Genetically modified humans: scientists in China have crossed one of biotechnology's red lines http://t.co/33WGpS2w3Z
10:20pm April 28th 2015 via Hootsuite
RT @EricTopol: The Hospital Microbiome Project http://t.co/hZawzjgRcy Add the elevator buttons http://t.co/mAQIriNjir http://t.co/psDVQUHB2r
9:25pm April 28th 2015 via Hootsuite
"For most people... WGS is an absurd medical test" - LA Times http://t.co/KIGNrTONsx HT @drbachinsky
8:50pm April 28th 2015 via Hootsuite
RT @drbachinsky: Integrative analysis of RNA, translation and protein levels reveals distinct regulatory variation across humans http://t.c…
8:36pm April 28th 2015 via Hootsuite
RT @moorejh: MT @FastCompany Tool tracks #socialmedia content to show how stories spread http://t.co/KadQx8n36C http://t.co/X7gL4abldd
7:30pm April 28th 2015 via Hootsuite
RT @tvansipe: 15 Secrets of Really Persuasive People http://t.co/o9AbJ9LNcj via @Entrepreneur
6:30pm April 28th 2015 via Hootsuite
Vessyl - do people >really< need a 'smart cup'? | YouTube http://t.co/5x9meYEzXL
5:30pm April 28th 2015 via Hootsuite
RT @tgoetz: Personalized medicine with genomics? Yup -try asking patients what they want. http://t.co/NmesVmXgop
4:10pm April 28th 2015 via Hootsuite in reply to tgoetz
RT @aeonmag: Now is a good time to revisit D. Watkins' piece on the racial divide in #Baltimore: http://t.co/ZpACz6LElf http://t.co/MigJtWH…
3:45pm April 28th 2015 via Twitter Web Client
DNA Phenotyping in Hong King to Discourage Litter | Ecozine http://t.co/bUsFeN3B95
3:40pm April 28th 2015 via Hootsuite
In Phys Rev Lett? Really? Newly Discovered Bacterial Crystals Are a Biophysics Mystery | Motherboard http://t.co/wJkS5WydPL
2:10pm April 28th 2015 via Hootsuite
