DP:ID of novel druggable targets for combination therapy: run parallel in-vitro and in-vivo shRNA screens #AACR15
10:34am April 18th 2015 via Twitter Web Client
RT @meissner_t: DP: copywriteR https://t.co/2UjMTlikXy off-target reads to improve CNV detection on exome seq #AACR15 no reference needed
10:31am April 18th 2015 via Twitter Web Client
DP:Showing MITF loss ass'd with in/extrinsic BRAF1 resisence; also seen in acquired resistance. AXL induced #AACR15
DP:Also copy-number alterations (CNA); CopywriteR works on either WGS or targeted sets (360 genes), reference-free #AACR15
10:26am April 18th 2015 via Twitter Web Client
DP: Showing pleiotropy in targeted drug resistance; several mutations ubiquitous, others shared, still others unique #AACR15
10:24am April 18th 2015 via Twitter Web Client
DP:Challenge is # of mutations; melanoma in particular from Alexandrov 2013 http://t.co/QG1EL0Fklx 30K mutations #AACR15
10:23am April 18th 2015 via Twitter Web Client
DP: Started with striking photos of individ. from Wagle 2011 paper, pretreatment, much better at 4mos, much worse at 6mos. #AACR15
10:21am April 18th 2015 via Twitter Web Client
Big data session: Daniel Peeper (Netherlands Cancer Inst) “Large-scene perturbations to reveal cancer dependencies” DP #AACR15
10:19am April 18th 2015 via Twitter Web Client
AT: CD47, MET, CD44+ EPCAM 'contain functional and transplantable Metastasis Initiating Cells'. Freq: 1%-44% of CTCs #AACR15
10:02am April 18th 2015 via Twitter Web Client
AT: Summary: CD44, MET, CD47 markers of metastatic stem cells; 12% of all tumor cells in aggressive disease #AACR15
9:58am April 18th 2015 via Twitter Web Client
AT: High-risk individs (>5CTCs/7.5mL) easier to get successful xenografts. #AACR15
9:54am April 18th 2015 via Twitter Web Client
AT: "Phenotype and biology of CTC's are almost a 'black box'" From br ca samples, enumerate, ID EPCAM and show metastatic potential #AACR15
AT: CellSearch has been 'instrumental in the last 20y'. Showed Meier-Kaplan for br ca, EPCAM and survival. http://t.co/ep02bYoYVv #AACR15
9:52am April 18th 2015 via Twitter Web Client
AT: They expect metastasis stem cell from CTCs. Fig from 2012 http://t.co/85AYTLR7N0 Distant met has to have stem-cell-like capacity #AACR15
9:51am April 18th 2015 via Twitter Web Client
AT: This 2014 ref http://t.co/K1EAUSfkoD figure - 'it's clear a combination of genetic and epigenetic factors' for CSCs #AACR15
9:49am April 18th 2015 via Twitter Web Client
AT: Refers to recent Vogelstein correlation between total stem cell divisions in smaller vs larger organs #AACR15
9:46am April 18th 2015 via Twitter Web Client
AT: Niche reprogramming - showed 5 major papers, one NEJM http://t.co/nqqgmmIEFg first mut's in normal HSCs #AACR15
AT: SC self-renewal combines proliferation & inhibition of differentiation. Epigenetic control of 3 fates via niche inhibition #AACR15
9:44am April 18th 2015 via Twitter Web Client
AT: View from 2001: Cancer stem cell figure from ref http://t.co/ra2g6e4JUa In 2015: Genetic and CSC hetergeneity (epistatic) #AACR15
9:43am April 18th 2015 via Twitter Web Client
AT: Adult tissue stem cells 'essential to maintain and repair regenrative tissues' Billion/day, 100g/week. #AACR15
9:41am April 18th 2015 via Twitter Web Client
Andreas Trumpp (German Cancer Ctr Heidelburg) "Malignant stem cells in the circulation" AT #AACR15
9:40am April 18th 2015 via Twitter Web Client
CD: Q about CTC's and COPD: "Evidence that CTC's leave the mother ship early in carcinogenesis" #AACR15
CD: Concludes with an individual with aggressive SCLC and its characterization. #AACR15
9:38am April 18th 2015 via Twitter Web Client
CD: Referred to this 2014 BMC Genomics http://t.co/jpx6GEowSV doing single-cell RNA-Seq Also data from scWES #AACR15
9:34am April 18th 2015 via Twitter Web Client
CD: PCA plot of CNV data from 6414 protein-coding genes from 3 individs - responsive, & 2 refractory; some trends observed #AACR15
9:33am April 18th 2015 via Twitter Web Client
CD: Cell-by-cell heterogeneity - 'we need to do more of this to understand it more fully'. #AACR15
9:31am April 18th 2015 via Twitter Web Client
CD: Can do single-cell sequencing; shows spec TP53 mutation in bulk. Laid out bioinformatic workflow; WBCs -ve control #AACR15
9:30am April 18th 2015 via Twitter Web Client
CD: DEPArray instrument is 'a big American fridge' (large); explains dilectric cages. Blog on BtB: http://t.co/7rkGgtS9tY #AACR15
CD: Their workflow - 'analyze w/in 4d' w/CellSearch or Parasortix. Biobank in glycerol. Can do cfDNA. CTC isol. w/DEPArray. #AACR15
9:28am April 18th 2015 via Twitter Web Client
CD: Looking at CDX models from individual samples, can look at copy-number: clear pattern of gain/loss between sensitive / non-sens #AACR15
9:26am April 18th 2015 via Twitter Web Client
CD: Showed difference between chemo-sens and chemo-refractory. Get CTC-Derived Explants (called CDX) in-vitro model #AACR15
9:25am April 18th 2015 via Twitter Web Client
CD: Can bank CTCs in glycerol - 'an important step for us' b/c of real time nature. 2014 Nature Med http://t.co/vK8DoRglVr #AACR15
9:23am April 18th 2015 via Twitter Web Client
CD: CellSearch - showed data from this 2012 JCO paper http://t.co/3qwailpeG6 #AACR15
9:21am April 18th 2015 via Twitter Web Client
CD: SCLC (20% of all lung ca): CTC research 'has a real role to play' since the biology is poorly understood, small tissue amounts #AACR15
9:19am April 18th 2015 via Twitter Web Client
CD: 'CTC research is not for the faint hearted'. Not plug-and-play. #AACR15
9:17am April 18th 2015 via Twitter Web Client
CD: Turnaround time? Real time analysis or bank? Cost? Bioinformatics? What to measure (DNA, RNA, protein)? #AACR15
CD: Which to choose? "It depends on what you want'. Is it marker-dependent? Sample coll? QC? Fixed? Single or pools of cells? #AACR15
9:16am April 18th 2015 via Twitter Web Client
CD: Vitatex is a functional selection (for invasiveness). Gilupi nano detector - looking for EpCAM. They use DEP Array & ImageStream #AA
9:15am April 18th 2015 via Twitter Web Client
CD: DEPArray, ImageStream for imaging. 'Marker-dep and indep approaches, larger blood volumes' #AACR15
9:14am April 18th 2015 via Twitter Web Client
CD: Figure of technical challenges - CellSearch, iChip, ISET, Cell Sieve, Spiral Chip, Parsortix, ALS, Vitatex. Then: DEP Array #AACR15
9:13am April 18th 2015 via Twitter Web Client
CD: Biopsies are challenging; 'serial biopsies pre- and post- more challenging still' Is enrichment good enough or pure needed? #AACR15
9:12am April 18th 2015 via Twitter Web Client
CD: Are CTC's representative of a tumor? May be overall tumor burden, and in particular - subset of aggressive subtypes. #AACR15
9:11am April 18th 2015 via Twitter Web Client
Caroline Dive (CRUK Manchester) ‘Circulating tumor cell biopsies’ - is there (still) an elephant in the room? #AACR15
9:10am April 18th 2015 via Twitter Web Client
Caught end of Muneesh Tewari (UMich) 'Circulating microRNAs'. Cited 2013 ref cp between ddPCR & qRT-PCR http://t.co/hs25p4Ar8m #AACR15
9:07am April 18th 2015 via Twitter Web Client
For the next 5 days expect a flood of #AACR15 tweets thru 4/22 from 7am-6pm all about #cancer http://t.co/eNLrb64DCu
9:00am April 18th 2015 via Hootsuite
RT @vocativ: Science says your altruism is just evolution trying to make you attractive http://t.co/dnKR6OKr6i http://t.co/ODNXRtDw40
8:15am April 18th 2015 via Hootsuite
RT @iGEM: Many thanks to @Google for their sponsorship of #iGEM2015!!
7:15am April 18th 2015 via Hootsuite
RT @omespeak: San Diego to be featured in Nat Geo's 'World's Smartest Cities' program http://t.co/BZSe4Ai4WT
6:55am April 18th 2015 via Hootsuite
RT @Lee_Flea: Lion Torrent at the National Zoo, South Africa. See what I did there :) #IonMiniTour @iontorrent http://t.co/IzLTcA3RTh
5:55am April 18th 2015 via Hootsuite
RT @Xilouw: Ion Torrent Mini driving through Golden Gate @LifeTech #ionminitour #Travel #nature http://t.co/r7LhzQqtkH
4:50am April 18th 2015 via Hootsuite
