Pfeifer: 1 week of fixation: no 'major difference' in % of mapped reads. Increased coverage var, lower unique reads #FDANGSWorkshop
9:13am February 25th 2016 via Twitter Web Client
Pfeifer: The reality: high concordance, only 1/10kb differences; but 100x higher depending inter-tumor heterogeneity #FDANGSWorkshop
9:12am February 25th 2016 via Twitter Web Client
Pfeifer: Also chemical artifacts, transitions (C -> U) rate of 0.0015 and 4x more common than fresh #FDANGSWorkshop
9:11am February 25th 2016 via Twitter Web Client
Pfeifer: Clinical FFPE tissue: Formalin fixation #FDANGSWorkshop https://t.co/oK0Q0CLWuT and looking at GC content
9:10am February 25th 2016 via Twitter Web Client
Pfeifer: #FDANGSWorkshop Points to this 2010 Nature fig https://t.co/cmT9as4zoE on heterogeneity and sampling
9:08am February 25th 2016 via Twitter Web Client
Pfeifer: Not only quant and qual of DNA, but 'something small called library complexity' #FDANGSWorkshop
9:06am February 25th 2016 via Twitter Web Client
Pfeifer: Starts on pre-analytical steps 'before step 1' of NGS. Gross processing, tissue proc, histopath review; DNA pur #FDANGSWorkshop
Schetter: Intro panelists John Pfeifer (WashU), Dara Aisner (U CO) M. Berger (MSK), R Luthra (MDA), M.Rossi (Emory U) #FDANGSWorkshop
9:05am February 25th 2016 via Twitter Web Client
Philip: Even sequencing platform differences, and effect on analytical validation #FDANGSWorkshop
9:02am February 25th 2016 via Hootsuite
Philip: And what validation needed to support claims. And analytical validation and bioinf: from CDx to VUS Q's on validity #FDANGSWorkshop
8:58am February 25th 2016 via Hootsuite
Philip: Seeking input on Pre-analytical and Quality Metric approaches. Which steps most critical? What tissue types hard? #FDANGSWorkshop
8:57am February 25th 2016 via Hootsuite
Philip: In Table 2: Include tissue types? What level of clin validity outside tables 1/2? Invites feedback to docket #FDANGSWorkshop
8:56am February 25th 2016 via Hootsuite
Philip: #FDANGSWorkshop Discussion paper PDF: https://t.co/HfazwulstZ has intended use statement. Table 2 intended use generates add'l Q's
8:54am February 25th 2016 via Hootsuite
Philip: Questions: what genes / vars to include? Limits of reporting? Matching blood? Units of validation? Difficult units? #FDANGSWorkshop
8:51am February 25th 2016 via Hootsuite
Philip: Entire system validation - from collection through generation of reporting; all performance char's #FDANGSWorkshop
8:50am February 25th 2016 via Hootsuite
Philip: Hypothetical case: 10-gene NGS panel; 5 CDx genes, DNA/RNA 100 SNVs, 10 indels, 50 fusions, 10 CNVs. CRC and NSCLC #FDANGSWorkshop
Philip: Intended use: CDx and alternative Rx. Today doesn't cover ctDNA (July 19th planned). No WES/WGS, carrier screening #FDANGSWorkshop
8:48am February 25th 2016 via Hootsuite
Philip: Today's goals: obtain stakeholder input to establ. performance char's for rare variant types; labeling, performance #FDANGSWorkshop
8:47am February 25th 2016 via Hootsuite
Philip: PDF of CDx guideline here: ://ow.ly/YK7Hv Reviews Clinical utility, validity. CDx to-date has been single-analyte #FDANGSWorkshop
8:45am February 25th 2016 via Hootsuite
Reena Philip starts #FDANGSWorkshop with a discussion of Personalized Med and companion diagnostic's role. CDx guidelines Aug 2014
8:42am February 25th 2016 via Hootsuite
#FDANGSWorkshop Begins - Next Gen Sequencing-Based Oncology Panels, 2/25/16 https://t.co/d06mPZl5bO https://t.co/cM51DzNfma
8:36am February 25th 2016 via Hootsuite
RT @atulbutte: Wow! Inova Health unveils $100 million venture fund focused on #precisionmedicine! https://t.co/Xefjk54eT1
7:45am February 25th 2016 via Hootsuite
RT @FDADeviceInfo: Public Workshop - Next Gen Sequencing-Based Oncology Panels, 2/25/16 https://t.co/6Or0w4Hxc4 #fda #medicaldevice
6:55am February 25th 2016 via Twitter Web Client
Illumina Sues Oxford Nanopore Technologies Over Composition of Nanopores - Bio-IT World https://t.co/XN444FyVV8
6:50am February 25th 2016 via Hootsuite
Register for Friday's noon ET / 5 GMT NGS Oncology Panels and the FDA Webinar | TOMABio @SeraCare https://t.co/EQuOFh5TFt
5:15am February 25th 2016 via Hootsuite
RT @Clive_G_Brown: With Patent Suit, Illumina Looks to Tame Emerging British Rival, Oxford Nanopore https://t.co/V3VICiYP7E
4:27am February 25th 2016 via Hootsuite
RT @pickover: First-Place Science Project. https://t.co/Tww2FO6ZEs
9:03pm February 24th 2016 via Hootsuite
Google's Verily, Dexcom aim to launch mini glucose monitor in 2018, with next-gen by 2021 - FierceMedicalDevices https://t.co/PkuTogACvS
8:15pm February 24th 2016 via Hootsuite
Why reporting on health and science is a good way to lose friends and alienate people - Vox https://t.co/ncE8ZGMfbz
6:10pm February 24th 2016 via Hootsuite
4:15pm February 24th 2016 via Hootsuite
Genes with monoallelic expression contribute disproportionately to genetic diversity in humans : Nature Genetics ($) https://t.co/Yp7B6QjroM
4:05pm February 24th 2016 via Hootsuite
Identification of neutral tumor evolution across cancer types : Nature Genetics ($) https://t.co/1Bo05BtwEi
3:01pm February 24th 2016 via Hootsuite
Single molecule nanopore ion evaporation MS as a sequencing method? Latest Clinical OMICs https://t.co/fBGuEKUE4m
1:00pm February 24th 2016 via Hootsuite
Epigenetic-Chronological Age Mismatch Warns of Cancer | ClinicalOMICs https://t.co/YLTIA2xtXp 71 methylation marks
12:50pm February 24th 2016 via Hootsuite
Illumina Sues Oxford Nanopore for Patent Infringement | GenomeWeb https://t.co/WJsUiRekV4
11:50am February 24th 2016 via Hootsuite
Foundation Medicine's Revenues Grow 39 Percent in Q4, Net Loss Widens | GenomeWeb https://t.co/TeLeLuv2An
10:36am February 24th 2016 via Hootsuite
Shots fired – nanopore wars part II | https://t.co/GmdjrQaWKU
9:20am February 24th 2016 via Hootsuite
The prognostic significance of KRAS mutation subtypes in lung adenocarcinomas in Chinese | OncoTargets and Therapy https://t.co/r7d8D0Vuwu
7:50am February 24th 2016 via Hootsuite
WEBINAR: Oncology Panels and the FDA Friday noon ET / 5pm GMT https://t.co/EQuOFh5TFt
6:19am February 24th 2016 via Hootsuite
Roche's Upcoming Heat-Seq Target Enrichment Product Promises Automation-Friendly Workflow | GenomeWeb ($) https://t.co/AHCyh5Y3dp
5:25am February 24th 2016 via Hootsuite
Tech of the Future | GenomeWeb https://t.co/T4nUSgkq2U
10:05pm February 23rd 2016 via Hootsuite
Drinking more coffee may undo liver damage from booze | Reuters https://t.co/yZYC8OGl02
9:50pm February 23rd 2016 via Hootsuite
RT @justinehausheer: A Guide to Scientific Crowdfunding https://t.co/lPwW21rkc9 new study from @PLOSBiology
8:00pm February 23rd 2016 via Hootsuite
Silicon Chip with Integrated Laser Paves Way for Data Processing with Light | Scientific Computing https://t.co/1Wnvxpg0ZU
7:15pm February 23rd 2016 via Hootsuite
Two Superpowers We Wish We Had | Bill Gates 2016 Annual Letter https://t.co/B5kIVkReW6
6:10pm February 23rd 2016 via Hootsuite
Extreme Numbers: The Unimaginably Large and Small Pop Up in Recent Experiments | Scientific Computing https://t.co/p7xeAfYi0Y
5:10pm February 23rd 2016 via Hootsuite
Confessions of a social engineer: "(People are) the weakest link in any company’s security" | Daily Dot https://t.co/XmYRXVIxdR
4:55pm February 23rd 2016 via Hootsuite
RT @NatureNews: Leaving academia: "I didn’t expect the process to be so emotionally difficult" https://t.co/Gi0yTq5RR5
3:01pm February 23rd 2016 via Hootsuite
Vienna named world's top city for quality of life | The Guardian https://t.co/1IF8oYyyE7
2:05pm February 23rd 2016 via Hootsuite
Air pollution, health and cancer — a new report calls for action — Medium https://t.co/WW2kY4ONzp
1:05pm February 23rd 2016 via Hootsuite
