Daber: Now w/RNA sequencing high res, hypothesis-free. #TRICON
6:07pm March 9th 2016 via Hootsuite
Daber: For background starts with BRC-ABL, imatinib. Started w/Chr9/22, low-res. Then specific hypothesis. RT-qPCR high-res, spec #TRICON
Bob Daber (BioReference) Best practices for fusion detection by targeted RNA sequencing - pre-analytical considerations #TRICON
6:05pm March 9th 2016 via Hootsuite
Velculescu: Shows data from '15 ref https://t.co/3UUeIEGGo6 in Panc Ca - 6 mos earlier detection possible via ctDNA cp to imaging #TRICON
2:56pm March 9th 2016 via Hootsuite
Velculescu: Detected ERBB2 amplification in plasma '12 ref https://t.co/y9acTWeuxr and rearrangements #TRICON
2:54pm March 9th 2016 via Hootsuite
Velculescu:Variability, but for late-stage pts widely observed. Many ways to detect - '10 ref https://t.co/xYdPT7uKau (used SOLiD!) #TRICON
2:53pm March 9th 2016 via Hootsuite
Velculescu: Study included 600 pts, CRC, breast, have high fx of patients with ctDNA. Others difficult (glioma, thyroid) #TRICON
2:51pm March 9th 2016 via Hootsuite
Velculescu: Beyond therapy: as tumors grow cells and DNA release into the bloodstream '14 ref https://t.co/ucufYDYBD6 #TRICON
2:49pm March 9th 2016 via Hootsuite
Velculescu: Challenges - accurate somatic mut detection; det mutation load; discriminate non vs responders; improve MHC-I prediction #TRICON
2:47pm March 9th 2016 via Hootsuite
Velculescu: Some pts have high mutation burden but don't response; also the opposite. Multiple layers to overcome applying immunoRx #TRICON
2:46pm March 9th 2016 via Hootsuite
Velculescu: Response rate lower in NSCLC PIII study in NEJM '15 https://t.co/kUUP19sivc Dev ranking for neoantigens and response #TRICON
2:45pm March 9th 2016 via Hootsuite
Velculescu: Shows waterfall plot for PD-1 blockade and mutation burden in mismatch repair genes '15 NEJM https://t.co/ps0MNdd4UV #TRICON
2:43pm March 9th 2016 via Hootsuite
Velculescu: Applications today: Immunotherapy and focus on neoantigens. Subset of lung/colon ca w/mismatch repair mut's #TRICON
2:41pm March 9th 2016 via Hootsuite
Velculescu: Tumor-only vs tumor-normal analysis: discovery of both somatic and germline var's #TRICON GenomeWeb: https://t.co/jLC78ZtjvE
2:40pm March 9th 2016 via Hootsuite
Velculescu: Now connection to immunotherapy: neoantigen work '08 ref https://t.co/blDnRAMHIa #TRICON
2:38pm March 9th 2016 via Hootsuite
Velculescu: New targets: shows figure from '15 ref https://t.co/zoEydDo1MM Majority have entry into clinical trials #TRICON
2:36pm March 9th 2016 via Hootsuite
Velculescu: (Discloses PGDx founder): discoveries from first 100 cancer genomes - the genetic landscape. PIK3CA, IDH1/2; ARID1A etc #TRICON
2:35pm March 9th 2016 via Hootsuite
Victor Velculescu (Hopkins) Cancer genome analyses in tissue and liquid biopsies #TRICON
2:33pm March 9th 2016 via Hootsuite
Q:People in/out of the system? A:Butte: Wants a 'sticky' system, and opt-out/in; possible to monitor via devices; also claims data #TRICON
2:32pm March 9th 2016 via Hootsuite
Butte:A2:Not to get rich, but to get this to patients. Not just writing papers. Stop thinking 'either or': a continuum of innovation #TRICON
2:31pm March 9th 2016 via Hootsuite
Q:Ability to bring to other palces? Butte:No real magic, academia hurts itself not talking about starting companies #TRICON
2:30pm March 9th 2016 via Hootsuite
Butte: 9 Institutes of NIH, support from HHMI, UCSF etc #TRICON
2:29pm March 9th 2016 via Hootsuite
Butte: An 'accountable care organization'. 'W/mobile devices and all the rest' - what the UC Health system transforms into. Amazing! #TRICON
2:28pm March 9th 2016 via Hootsuite
Butte: Mean age, notes, colors, dots: what will happen, and what we will do about it to prevent that disease/death. #TRICON
2:27pm March 9th 2016 via Hootsuite
Butte: 2019 new facility: imagines a map of disease, similar to Google Maps. A map of death and disease- awesome animation #TRICON
Butte: It is the EDW for the entire UC Health system. 14.1M pts, 4% of the US pop, putting all the data into a ReX explorer db #TRICON
2:24pm March 9th 2016 via Hootsuite
Butte: "I'm slumming it out in this brand-new building, as they are getting ready another brand-new building" at UCSF; UC Health #TRICON
2:23pm March 9th 2016 via Hootsuite
Butte: NuMedii now chasing down the biology. $3.5M funding, Allergan 'taps digital discovery platform for psoriasis R&D' #TRICON
2:22pm March 9th 2016 via Hootsuite
Butte: Psych anti-depressant imipramine - activity against SCLC - shown in triple knockout mouse model. Credits Joel Dudley #TRICON
2:19pm March 9th 2016 via Hootsuite
Butte: Looking at gene correlation and drug effect. No cause, no cure, just treatment. Seizure drug for Crohn's - repositioning #TRICON
2:18pm March 9th 2016 via Hootsuite
Butte: Now for drug discovery - datasets w/ and w/o drug treatment. Diseased vs healthy. "A https://t.co/EizDlUTK8M for drugs" #TRICON
Butte: "I'm giving away secrets here" b/c the need is so great. #TRICON
2:17pm March 9th 2016 via Hootsuite
Butte:$2M in funding - not to brag, but Carmenta funded by private dollars. https://t.co/G1f8yCgyLU acquired https://t.co/POm9wW5PUN #TRICON
2:16pm March 9th 2016 via Hootsuite
Butte: Anything in common? Chased down as protein form: #TRICON '13 ref https://t.co/5fQGQ4rYFf via March of Dimes
2:15pm March 9th 2016 via Hootsuite
Butte: Preeclampsia - how husbands lose wives around the world. 5-8% of all pregnancies, search db's: hundreds of samples present #TRICON
2:14pm March 9th 2016 via Hootsuite
Butte: "RNA is funny, it'll degrade if you look at it. I like protein diagnostics." #TRICON
2:13pm March 9th 2016 via Hootsuite
Butte: A few stories: Looking at 'every human disease', calculate signatures for diagnostics, blood tests. #TRICON
2:12pm March 9th 2016 via Hootsuite
Butte: 'A case of retroactive crowdsourcing" - as a newbie, you have 2600 researchers already helping you. #TRICON
2:11pm March 9th 2016 via Hootsuite
Butte: And 2600 investigators have deposited br ca data. GEO datasets via NCBI https://t.co/ljUF9Yqvco #TRICON
2:10pm March 9th 2016 via Hootsuite
Butte: What does it mean? Anyone can search, even a student doing a science fair project. 66K br ca of gene exp data available #TRICON
2:09pm March 9th 2016 via Hootsuite
Butte: Req'ment for public deposition of data: milestone in Aug '12 - doubling every 3-4y. Then 1M - now 2M samples #TRICON
2:08pm March 9th 2016 via Hootsuite
.@atulbutte showing at #TriCon the formats of Affymetrix microarrays to 384-wells https://t.co/nMRweROIL0
2:07pm March 9th 2016 via Hootsuite
Butte: (Love hearing @atulbutte present - both entertaining and informative.) #TRICON
2:02pm March 9th 2016 via Hootsuite
Butte: "Physicians laugh at this" - code for spacecraft crashing (if a passenger) V97.33XD meme - getting sucked into a jet engine! #TRICON
Butte: #PMINetwork Initiative - how do you classify medicines? Diseases? ICD now in the 10th ed (started in the 1870s!) #TRICON
2:01pm March 9th 2016 via Hootsuite
Butte: 8 of his students have started co's- Flipora, MyTime, Praedicat, NunaHealth, Stimulomics, Serendipity, Carmenta, Numedii #TRICON
1:59pm March 9th 2016 via Hootsuite
Butte: 'Translating a trillion points of data into therapies and new insights in health and disease' Huge list of conflicts! #TRICON
1:58pm March 9th 2016 via Hootsuite
Atul Butte (UCSF) "Precision Medicine and Cancer Drug Discovery" @atulbutte #TRICON
1:57pm March 9th 2016 via Hootsuite
Q:Long Q abt parsimony and personalized medicine A:Andruss Binary decisions for many, but a long tail for the rest #TRICON
12:34pm March 9th 2016 via Hootsuite
Beechem: Not an Ab co, resolution about 6x6 cells, <100um resolution. Aiming for single-cell #TRICON
12:29pm March 9th 2016 via Hootsuite
