Q:What about pre-analytic var's for NanoString? A:Beechem:Not trying 'big iron MS shops' but getting in labs; barcoding not spectral #TRICON
12:29pm March 9th 2016 via Hootsuite
Beechem: A lot of add'l information downstream. KOLs in immuno-oncology really wanted this capability #TRICON
12:19pm March 9th 2016 via Hootsuite
Pothier: "I want that NanoString ability: merging a lot of tech; will it replace or add-on?" Beechem: Merges IHC and profiling #TRICON
12:18pm March 9th 2016 via Hootsuite
Garlick: Advantage of biosynthetics, can adapt controls to changing field, and work with IVD manufacturers #TRICON
12:15pm March 9th 2016 via Hootsuite
Garlick: During assay design, want to challenge the assay (detection limits, breadth of variation) to make sure the assay working #TRICON
12:14pm March 9th 2016 via Hootsuite
Pothier: SeraCare is in 'we need to have that category.' How do you work as a customize-able fashion" A:Formulates custom products #TRICON
Andruss: Developed a Sanger-based method, and software for specialized analysis called AmplideX FMR1 Reporter SW #TRICON
12:05pm March 9th 2016 via Hootsuite
Andruss: Can go to RNA, leverage to companion Dx, expand content. Onto Fragile X: CGG repeat size, methyl status, AGG status; FMR1 #TRICON
Andruss: Their QuantideX NGS Pan Cancer Kit, integrates sample QC, controls, library prep, informatics (DNA) #TRICON
12:02pm March 9th 2016 via Hootsuite
RT @AMcMAssociates: #tricon Russell garlic tells us Only 10-20% cancer patients get tumour genetic profiling - shocking! #tricon @seracare
12:01pm March 9th 2016 via Hootsuite
Andruss: How hard to run? Expertise? Interpretation? Workflow? Asuragen provides 'complete systems' incl. FDA clearance strategy #TRICON
12:00pm March 9th 2016 via Hootsuite
Bernie Andruss (Asuragen) Enhancing adoption of new diagnostic technologies #TRICON
11:59am March 9th 2016 via Hootsuite
Beechem:Summary "Technology much be simple/robust enough to be delivered to the clinic". Keytruda, anti-PD1, deal w/Merck #TRICON
11:57am March 9th 2016 via Hootsuite
Beechem:(NB - this is amazing IMHO - looking at SNVs by spatial location, mRNA by location; protein by location in FFPE...) #TRICON
Beechem:360nm light illuminates, sip the cleaved barcodes, and measure. Then move the objective; can look at '3D' via this system! #TRICON
11:56am March 9th 2016 via Hootsuite
Beechem: Label FFPE slide w/ Ab mix, illuminate region of interest; made the barcodes linked to Ab w/cleavable linker #TRICON
11:55am March 9th 2016 via Hootsuite
Beechem: Spatial representation - at #AACR16 will show a 'molecular-profiling microscope' by overlay of optical barcodes #TRICON
11:54am March 9th 2016 via Hootsuite
Beechem: showing mRNA plus protein modeling: NK, CD8+, DC cell types by Z-score shown #TRICON
Beechem: Input is 5K to 100K cells; shows melanoma data changes linked to genotype (+/- TKI phosph. status) w/mRNA pathways #TRICON
11:53am March 9th 2016 via Hootsuite
Beechem: 5ng DNA; 50ng for mRNA; 500ng for protein. Non-amplified readout, simultaneous detection of all 3 analytes, 12 lanes/cart #TRICON
11:51am March 9th 2016 via Hootsuite
Beechem: CTLA-4 and PD-1/PD-L1 has a small peptide; he came from @appliedbio to develop 'optical barcode everything' #TRICON
11:50am March 9th 2016 via Hootsuite
Beechem: Immuno-onology is 'systems biology at work'. Understand host, tumor biology, microbiome, and immune system interaction #TRICON
Joe Beechem (NanoString) 3D Biology simultaneous DNA, RNA and protein analysis from limiting sample #TRICON
11:49am March 9th 2016 via Hootsuite
Garlick: (Mentions 3pm talk today at #TRICON ) Summary- multiplexed controls allows pooling of data, ability to monitor across time, labs
11:48am March 9th 2016 via Hootsuite
Garlick: Visual chart cross labs for outliers (95% binomial), 3/4 on Ion Torrent around 5%; 1/3 ILMN labs around 5% #TRICON
11:46am March 9th 2016 via Hootsuite
Garlick: 18% allele freq; 10% allele freq; 26 var's per assay. Can draw true comparisons. Crossplatform; outlier analysis shown #TRICON
11:45am March 9th 2016 via Hootsuite
Garlick: Multi-lab study: NCI-Frederick, Dartmouth, BioReference; Weill Cornell; Jackson Labs. 357 assays/8 weeks #TRICON
11:44am March 9th 2016 via Hootsuite
Garlick: Take-home: "Highly multiplexed assays require highly multiplexed controls" #TRICON
Garlick: SeraCare started w/seroconversion panels for HIV; immunoassays; multiplexed HBsAg. Today: precision diagnostics #TRICON
11:43am March 9th 2016 via Hootsuite
Garlick: We are still 'crossing this chasm' from developers to medical centers. At FDA mtg - only about 10% getting gene testing #TRICON
11:42am March 9th 2016 via Hootsuite
Garlick: Many uses of NGS, but with new tech, need new approaches to quality control. Starts with 'Crossing the Chasm' tech adoption #TRICON
11:41am March 9th 2016 via Hootsuite
Russell Garlick (SeraCare) New approaches to next-generation sequencing QC #TRICON
11:40am March 9th 2016 via Hootsuite
Chenchik: Their 'multiplex RNAseq' apparently does higher dynamic range cp to RNAseq #TRICON
Chenchik: RT-qPCR - up to 100,000 dynamic range; but doesn't scale to expressed genes. RNA-Seq/Microarray typically 1,000 dyn rnge #TRICON
11:38am March 9th 2016 via Hootsuite
Chenchik: Tool enables enumeration of immune cells: shows chart of # of expressed genes - dynamic range of gene expression tech #TRICON
11:37am March 9th 2016 via Hootsuite
Chenchik: Cellecta has built tool ImmuneNet for gene profiling of immune cell-types. RNAseq of 2500 immmune-assoc'd genes #TRICON
11:36am March 9th 2016 via Hootsuite
Chenchik: Reviews unique gene expression from GTeX project (overview at https://t.co/j9lfUnDr0r https://t.co/4A29jWwfPu ) #TRICON
11:35am March 9th 2016 via Hootsuite
Chenchik:(2011 Hanahan ref on Hallmarks of Cancer Revisited: https://t.co/VY5GqeysLB ICYMI) #TRICON
11:34am March 9th 2016 via Hootsuite
Alex Chenchik (Cellectis) Starts w/systems biology of cancer; microenvironment's role (uses diagram from Hallmarks of Cancer) #TRICON
11:32am March 9th 2016 via Hootsuite
Coker: Tech from search engines, evidence-based rules/ontologies, automatic data acquisition, machine learning, system training #TRICON
11:29am March 9th 2016 via Hootsuite
Coker: 'Massive scale semantic processing' - goal to provide high-volume/high quality interpretation (currently 7-10 d, manual) #TRICON
Coker: Time cover story Mar 30 '15 'Closing the Cancer Gap': two women on the cover, two different stories, different outcomes #TRICON
11:26am March 9th 2016 via Hootsuite
Coker: 3% of adult cancer pts enrolled in clinical trials; 6% enroll on-time. At the end, 'we're failing patients' #TRICON
11:25am March 9th 2016 via Hootsuite
Kevin Coker (Molecular Match) Clinical intelligence on demand; out of MD Anderson #TRICON
11:23am March 9th 2016 via Hootsuite
Lim: Pt sampling pre-, during and post-treatment, comparing cultured cells downstream via RNA/DNA analysis #TRICON
11:22am March 9th 2016 via Hootsuite
Lim: Can propagate rare CTCs, also complex immune cells. Launching 2 clinical trials in the fall w/UCSF #TRICON
11:21am March 9th 2016 via Hootsuite
Lim: Hypoxia and pressure - mimic the environment in a fibrotic tumor; pressure affects growth '11 ref https://t.co/Zlwyo6Ajs2 #TRICON
11:20am March 9th 2016 via Hootsuite
Lim: Developed 3D collagen-based hydrogels; xeno-free, chemically defined culture media; and a novel bioreactor (02 and pressure) #TRICON
11:18am March 9th 2016 via Hootsuite
Lim: Cell have adapted over 60y to plastic, animal serum, incubator conditions. Feel the future are patient-derived cells #TRICON
Lim: HelLa has been used for 60y; HPV discovery awards Nobel in '08; vaccine approved in '06 #TRICON
11:16am March 9th 2016 via Hootsuite
