Fleischhacker: Has posters TT51 (Sat AM) and OTH10 (Sat PM) Q: Streck and methylation inability? A: Unknown #AMP2016
8:44am November 9th 2016 via Hootsuite
Fleischhacker: Showed 7d at RT didn't lead to gDNA contamination, cp to Streck. ccfDNA quant and integrity didn't change #AMP2016
8:43am November 9th 2016 via Hootsuite
Fleischhacker: Showed decline in quant cp EDTA to PAX; both DNA and methylation assays. Shows similar data via QIAsymphony purif. #AMP2016
8:41am November 9th 2016 via Hootsuite
Fleischhacker: Used ERV-3 single-copy TaqMan, both 79bp and 297bp. Methylation assayed via b-actin + mSHOX2 duplex TaqMan #AMP2016
8:37am November 9th 2016 via Hootsuite
Fleischhacker: PAXGene is designed for both manual and automated purification; shows comparison to EDTA tubes in duplicate #AMP2016
8:36am November 9th 2016 via Hootsuite
Fleischhacker: Also minimize freeze/thaws, -80C storage. Onto PAXgene Blood ccfDNA - reviews claims of stability, draw-tube #AMP2016
8:34am November 9th 2016 via Hootsuite
Fleischhacker: Spin twice (low/hi speeds); storage lower=better; most applications plasma better than serum; use dedicated kits #AMP2016
8:31am November 9th 2016 via Hootsuite
Fleischhacker: Freeze/thaws should be limited to 3 or less. Major points: min hemolysis, separate cells w/in 6-8h. Store in EDTA #AMP2016
8:30am November 9th 2016 via Hootsuite
Fleischhacker: Shows data fig from Devonshire '14; spread via qPCR of 7 ref genes. Copies/mL appears consistent across 17 donors #AMP2016
8:29am November 9th 2016 via Hootsuite
Fleischhacker: Worth 'playing around' with pur/elution conditions - 3x difference in yield '15 ref https://t.co/QKjyRybGbq #AMP2016
8:27am November 9th 2016 via Hootsuite
Fleischhacker: Purification comparison '14 ref https://t.co/Izdu8EE2mI 4 dedicated kits - QIAamp came out on-top #AMP2016
8:26am November 9th 2016 via Hootsuite
Fleischhacker: 8h not much diff w/Heparin, Citrate, EDTA. After 1d, only EDTA (RT storage) #AMP2016
8:24am November 9th 2016 via Hootsuite
Fleischhacker: Nuclease activity in serum about 15x plasma. EDTA confers protection. #AMP2016
8:22am November 9th 2016 via Hootsuite
Fleischhacker: Shows data for 4y storage for pre-natal Dx at -20C. For ctDNA -80C '05 ref https://t.co/hv1snoIqm5 #AMP2016
RT @SeraCare: Clinical Laboratories: You Are Not Alone. #AMP2016 #NGS https://t.co/YDHZgOlpmG by @seracare https://t.co/Aby8O19rzm
8:18am November 9th 2016 via Hootsuite
Fleischhacker: Best method - '01 ref https://t.co/JwmdUNpsRd best is low-speed spins, not Percoll #AMP2016
8:16am November 9th 2016 via Hootsuite
Fleischhacker: Wong Clin Chem '13 ref https://t.co/MKpQj7IJmL shows NIPT effect w/Streck. ctDNA ref https://t.co/28hl7jayXF #AMP2016
8:14am November 9th 2016 via Hootsuite
Fleischhacker: '03 ref https://t.co/5bvygHiOXr 8h at RT 'no effect' via beta-globin conc. Use of Streck for NIPT - no diff at 2d 4C #AMP2016
8:13am November 9th 2016 via Hootsuite
Fleischhacker: Rasmussen '16 ref https://t.co/jDiCmiwVyT Tourniquet stasis, other var's showing no effect on ccfDNA #AMP2016
8:10am November 9th 2016 via Hootsuite
Fleischhacker: Mentions CNAPS european mtg. SPIDIA is EU for clin dx stds for pre-analytic steps https://t.co/CtdmixtrP8 #AMP2016
8:08am November 9th 2016 via Hootsuite
Fleischhacker: ccfDNA discovered in '48. Heitzer '15 ref https://t.co/eT3NLo6deu standardized pre-analytical mthds #AMP2016
8:07am November 9th 2016 via Hootsuite
Fleischhacker: (rest of title) "... the solution for a long-standing pre-analytical issue? (in liquid biopsy)" #AMP2016
8:05am November 9th 2016 via Hootsuite
First-up: @QIAGEN and PreAnalytiX ccfDNA. Dr. Michael Fleischhacker in Univ Halle/Saale Germany. 'PAXgene blood ccfDNA...' #AMP2016
8:04am November 9th 2016 via Hootsuite
For those listening in on #AMP2016 over Twitter - stay tuned, today's a full day of corporate workshops. @RocheDiaUSA @QIAGEN @iontorrent
8:02am November 9th 2016 via Hootsuite
Good morning #AMP2016 - @seracare has a workshop at 9am Rm 217 about NGS-based assay validation and QC.
8:00am November 9th 2016 via Hootsuite
Illumina Launches TruSight Tumor 170 | Yahoo Finance https://t.co/YNFnUwXbX4
2:50pm November 7th 2016 via Hootsuite
Illumina Submits Companion Dx to FDA; China Business Flourishes Despite Disappointing Quarter | GenomeWeb ($) https://t.co/2NGvJ3zdrA
1:50pm November 7th 2016 via Hootsuite
Guardant Health Expands Guardant360 Panel, Reports Increased Sensitivity | GenomeWeb https://t.co/tUaEGEHr88
11:50am November 7th 2016 via Hootsuite
Newborn Carrier Screening May Cause Confusion For Parents, Study Finds | GenomeWeb https://t.co/uwrXdHwpu4
10:35am November 7th 2016 via Hootsuite
Media’s Next Challenge: Overcoming the Threat of Fake News - The New York Times https://t.co/RIdfCLKKKN
9:39am November 7th 2016 via Hootsuite
Here’s the America Trump doesn’t get - The Washington Post https://t.co/fMkHS90pzv
6:40am November 7th 2016 via Hootsuite
Why you shouldn’t organize your email - The Washington Post https://t.co/9n8iEm2DKM
10:30am November 6th 2016 via Hootsuite
Join me in supporting National Capital Area Council Boy Scouts of America @AmazonSmile. #YouShopAmazonGives https://t.co/hFKoPy922V
8:01am November 6th 2016 via Twitter Web Client
How the Internet Is Loosening Our Grip on the Truth - The New York Times https://t.co/3XnCx8b9hi
8:45pm November 2nd 2016 via Hootsuite
On Wall Street, a high-ranking few still avoid email | Reuters https://t.co/UMSPuhQhm6
7:29pm November 2nd 2016 via Hootsuite
