Panel session Companion Dx: Where is the incentive? Mya Thomae Illumina, Sheila Walcoff Goldbug Strategies, Karen Heichman ARUP #PMWC17
7:04pm January 25th 2017 via Twitter Web Client
Weissman: Idea is to use 'dead' CRISPR/Cas9 to turn on or off genes. Target the RNA but no cleavage event. https://t.co/CDKyfIPHee #PMWC17
5:00pm January 25th 2017 via Twitter Web Client
Jonathan Weissman (UCSF/HHMI) Controlling the volume of gene expression with CRISPRi and CRISPRa #PMWC17
4:57pm January 25th 2017 via Twitter Web Client
Shafer: For acknowledgements, puts up the @ScienceMarchDC to advocate a cause. #PMWC17
Shafer: Did lentiviral vs synthetic RNA comparison for screening. Problem: RNA lasts only about 48h. #PMWC17
4:54pm January 25th 2017 via Twitter Web Client
Shafer: Sanger Inst generated a whole-genome arayed lentiviral CRISPR knockout for screening. #PMWC17
4:53pm January 25th 2017 via Twitter Web Client
Shafer: 6-GT treatment, and shows top genes up and down regulated; beat to publication by the Broad https://t.co/HCPirC13jI #PMWC17
4:50pm January 25th 2017 via Twitter Web Client
Shafer: Slew of papers with this concept in '14 Nature Biotech https://t.co/Ply0M740qi #PMWC17
4:48pm January 25th 2017 via Twitter Web Client
Shafer: Adv of stable integration via lentivirus: can select for CRISPR components; persistent, stable of gRNA; unique gRNA barcode #PMWC17
4:46pm January 25th 2017 via Twitter Web Client
Shafer: 17-21 bases needed for RNA complementarity, can be used as a screening tool w/1000's or 100's of 1000's of targets #PMWC17
Shafer: NHEJ can have a wide variety of indels, a random repair mechanism. Pooled lentiviral CRISPR knockout screening. #PMWC17
4:45pm January 25th 2017 via Twitter Web Client
Shafer: Mechanisms can be Homol-dep, or non-homol end joining (NHEJ). Look for the first 2/3 of the ORF of coding seq's #PMWC17
4:44pm January 25th 2017 via Twitter Web Client
Shafer: Before, RNAi was the way to do knockdown expts. With genome editing, can get knockouts. #PMWC17
4:43pm January 25th 2017 via Twitter Web Client
Shafer: Timeline for genome editing tools: Targetron in 2002; ZFNs in 2007; TALs in 2009; CRISPR 2012. #PMWC17
4:42pm January 25th 2017 via Twitter Web Client
Shawn Shafer (Millipore) CRISPR Screening - from GeCKO to Sanger #PMWC17
Doudna: Longstanding collaboration w/Roche, MPI Berlin Charpentier lab #PMWC17
4:41pm January 25th 2017 via Twitter Web Client
Doudna:Last summer, first clinical trial announced. https://t.co/ohAq9QICaa Many more expected. #PMWC17
4:40pm January 25th 2017 via Twitter Web Client
Doudna:Expanded toolbox figure from '16 Nature Biotech https://t.co/pKciXaIwAU #PMWC17
4:39pm January 25th 2017 via Twitter Web Client
Doudna:Cas9 RNPs for editing the brain: fig from Staahl et al submitted Can inject pre-assembled complexes, localized in mice brain #PMWC17
4:37pm January 25th 2017 via Twitter Web Client
Photo of DNA/RNA hybrid w/Cas9 based upon '14 Nature 3D structure https://t.co/2JCjKBOfoJ Add'l enzymes with other functions #PMWC17
4:35pm January 25th 2017 via Twitter Web Client
Doudna: A powerful technology not only for somatic cells but also in the germline. Shows a 3D printed model of Cas9 #PMWC17
4:33pm January 25th 2017 via Twitter Web Client
Doudna: Can work in plants, fungi, other bacteria. Challenges: delivery, controlling repair pathways, ethics #PMWC17
Doudna: Evolved in bacteria, but proteins with RNA-guided DNA cutting can handle eukaryotic histone organization #PMWC17
4:31pm January 25th 2017 via Twitter Web Client
Enjoyed lunch here at #PMWC17 with the one-and-only @atulbutte and @iGenomics - engaging discussion on data re-use. How needed!
Doudna: Shows a animated video on guide RNAs and Cas9; as an RNA-guided system, site-spec cleavage #PMWC17
4:29pm January 25th 2017 via Twitter Web Client
Doudna: Started with curiosity about how bacterial fight viruses #PMWC17
4:27pm January 25th 2017 via Twitter Web Client
Jennifer Doudna (UC Berkeley) CRISPR Technology: Impacts and challenges of DNA Editing #PMWC17
Avey: Cancer became a genetic problem, because of sequencing. #PMWC17
3:32pm January 25th 2017 via Twitter Web Client
Avey: The Broad and others are following the tech - part of the problem. So much other information to focus on. #PMWC17
3:31pm January 25th 2017 via Twitter Web Client
Reid: The stakes are so high, the knowledge is so low, the gap is so large... Molecular biology cp to software is so complex. #PMWC17
Avey: So many 23andMe customers never came back. Access the report and they were gone. It would be great if Helix can succeed. #PMWC17
3:30pm January 25th 2017 via Twitter Web Client
Q:(Helix engineer) Foresee Angry Birds for genetics? A fun experience? Avey: Goes back to UIX, Helix's platform very interesting. #PMWC17
3:29pm January 25th 2017 via Twitter Web Client
Q:What if pts don't really care? Billing: Alternative truth? What if people choose to believe w/o any basis? #PMWC17
3:28pm January 25th 2017 via Twitter Web Client
Billings: What part of the old system to preserve? Hagenkord: Band-aid over band-aid, I participate in many of the societies. #PMWC17
3:26pm January 25th 2017 via Twitter Web Client
Hagenkord: Healthcare, insurance, 'will buckle under its own weight'. And we're at an exciting time. #PMWC17
3:25pm January 25th 2017 via Twitter Web Client
Avey:Can go back to old medicine, changes in diet. Hagenkord: Pt-powered research will rock foundation of fundamentals of medicine #PMWC17
Reid: Antidepressants... Avey: B12 community aware their problem is w/metabolism. No std of care, will drive conversation #PMWC17
3:24pm January 25th 2017 via Twitter Web Client
Avey:What's legal will become less-used; what's illegal (w/microdosing). As a society - too medicated, ineffective #PMWC17
3:23pm January 25th 2017 via Twitter Web Client
Avey: Will see blurring between legal and illegal drugs; mimicking clinical trials. In US weird boundaries. #PMWC17
3:22pm January 25th 2017 via Twitter Web Client
Hagenkord: Some legal precedent - 'duty to warn'. #PMWC17
3:21pm January 25th 2017 via Twitter Web Client
Reid: Where in the world? Billings: Australia there's some mandatory reporting. Perhaps other Commonweath countries. #PMWC17
3:20pm January 25th 2017 via Twitter Web Client
Reid: GINA doesn't apply to life insurance. Will it change? Billings: Will need reform, having been personally involved. #PMWC17
3:19pm January 25th 2017 via Twitter Web Client
Billings: It may be led by the life insurance industry, to know more about genetics. #PMWC17
3:18pm January 25th 2017 via Twitter Web Client
Billings: Predicts ability to communicate within families is fracturing. Some kind of mandatory reporting (inf disease) expected. #PMWC17
Reid: At Complete he worked on it - the barriers are so high, while the effort for databases is laudatory, he's pessimistic #PMWC17
3:17pm January 25th 2017 via Twitter Web Client
Reid: So many barriers - financial, social, personal. 'Another mechanism' - thus he's working on solving that #PMWC17
3:16pm January 25th 2017 via Twitter Web Client
Reid: Interest groups sequencing, phenotyping larger collections, no federated databases. Predict: will fail #PMWC17
3:15pm January 25th 2017 via Twitter Web Client
Avey: Make it quick and convey complex information? A design problem. #PMWC17
3:13pm January 25th 2017 via Twitter Web Client
Q: Can you trust these results? Avey: Have to dig into details of reports (i.e. how many click thru the science behind the result) #PMWC17
Comment from a 15y ca survivor:Life in LA, taking CoQ10. People don't understand aspects of mammography tests, PSA test. #PMWC17
3:12pm January 25th 2017 via Twitter Web Client
