Patel: Another '14 NEJM https://t.co/vv4InWAmHp Clonal hematopoesis may cause mutations to appear in healthy individuals #MDxEU17
4:17am April 11th 2017 via Hootsuite
Patel: KRAS mutants exist in pre-cancerous lesions in lung tissue, for example. No good answer. '14 NEJM https://t.co/LhrEDOm2VW #MDxEU17
4:15am April 11th 2017 via Hootsuite
Patel: Do early-stage tumors release sufficient DNA? Are the same muts found in healthy individuals? #MDxEU17
4:14am April 11th 2017 via Hootsuite
Patel: Background error correction must be employed, as well as broad mutation coverage; biological challenges remain #MDxEU17
4:13am April 11th 2017 via Hootsuite
Patel: Tumor-derived biomarker portion is in the context of (relatively) high background; ctDNA is, in principle, only frm tumor #MDxEU17
4:11am April 11th 2017 via Hootsuite
Patel: His focus today - treatment response assessment, and early detection. Protein biomarkers - PSA for everyone is non-zero #MDxEU17
4:10am April 11th 2017 via Hootsuite
Patel: Expertise in therapeutic radiology. Non-invasive genotyping, resistance mutations, residual disease, disease recurr... #MDxEU17
4:09am April 11th 2017 via Hootsuite
Abhijit Patel (Yale Univ CT) Early detection using cfDNA: promise and challenges #MDxEU17
4:05am April 11th 2017 via Hootsuite
Helen Winter (Univ Oxford UK) Into at #MDxEU17 - two themes, early detection, and pt selection/stratification. Real challenge: translation
RT @NatureNews: The DIY electronics transforming research, from Raspberry Pi to Arduino: https://t.co/X5zauDxCbz https://t.co/Uza0aUsLC2
4:02am April 11th 2017 via Hootsuite
@larshaakon Hi - this was only her summary of #AACR17 likely from this plenary https://t.co/2sELB2zsN4 which I didn… https://t.co/WgO0MvgiKo
11:11am April 10th 2017 via Hootsuite in reply to larshaakon
Bernal: Onto proteomic markers; shows volcano plot to find statistical sig by magnitude of change, in serial in-labor plasma #MDxEU17
11:04am April 10th 2017 via Hootsuite
Bernal: There is a connection between infection and pre-term birth, but prostaglandin as a surrogate marker may be a red herring #MDxEU17
Bernal: #MDxEU17 Early excitement about accurate tests https://t.co/KhTiqGhAbi all have same problems of low PPV
10:58am April 10th 2017 via Hootsuite
Bernal: #MDxEU17 Some metabolic work in '10 https://t.co/SAuC8QnQu2 Shows summary predictive value of 'normal' preg https://t.co/NsLsS1Tn1D
10:57am April 10th 2017 via Hootsuite
Bernal: In human labor, no drop in progesterone. Not yet discovered yet in humans. Known a fetal zone w/fetal adrenal gland #MDxEU17
10:54am April 10th 2017 via Hootsuite
Bernal: Fetus and mother 'work together' as a maternal-fetal-placental unit. Conjugated androgens prod by placenta - DHA-S #MDxEU17
10:53am April 10th 2017 via Hootsuite
Bernal: Another risk factor - prev pre-term birth. Summarizes this section w/heterogeneous causes of preterm birth #MDxEU17
10:51am April 10th 2017 via Hootsuite
Bernal: And even passive (second-hand) smoke. Mechanisms: could be hypoxia of fetus, risk of membrane rupture. #MDxEU17
10:47am April 10th 2017 via Hootsuite
Bernal: #MDxEU17 15K women - showed active smoking and pre-term birth, one of few preventable causes '15 ref https://t.co/y4cFwjl93b
10:45am April 10th 2017 via Hootsuite
Bernal: Etiology - 45% is spontaneous. '10 ref https://t.co/QnLJlQ1bYe shows socioeconomic factors - tobacco, 'social problems' #MDxEU17
10:44am April 10th 2017 via Hootsuite
Bernal: #MDxEU17 Uses definitions from this '08 ref https://t.co/k1TKPSJs8d Very pre-term ~32w. Shows survival limit at 24w
10:42am April 10th 2017 via Hootsuite
Bernal: Cervix changes as well - a hard organ softening driven by matrix metallopoteinases (MMP-9) #MDxEU17
10:39am April 10th 2017 via Hootsuite
Bernal: Myometrial contractility via biochemistry, and ion channels regulating action potential w/calcium build-up. #MDxEU17
10:38am April 10th 2017 via Hootsuite
Bernal: 7M children <12yo are affected by preterm birth. Shows a painting from 1300's of Eve being extracted from Adam for context #MDxEU
10:37am April 10th 2017 via Hootsuite
Bernal: Uterus is a remarkable organ, also hard to study - due to changes in the smooth muscle in pregnancy. Yet contracts in labor #MDxEU17
10:36am April 10th 2017 via Hootsuite
A Lopez Bernal (Univ Bristol UK) Risk factors and proteomic biomarkers for pre-term labour #MDxEU17
10:34am April 10th 2017 via Hootsuite
Q: Number of women to treat via aspirin to prevent 1 case? Meiri: For high-risk, it is 1:10 (1 response for 10 treated) #MDxEU17
10:33am April 10th 2017 via Hootsuite
Q: Prevalence of mutation freq w/in ethnicities? Meiri: Black females in SA vs UK - different tribal assoc'ns #MDxEU17
10:32am April 10th 2017 via Hootsuite
Meiri: Shows in-vivo affect of WT PP13 protein admin in a rat model, cp to truncated PP13 '16 ref https://t.co/fTAf5FtQ5O #MDxEU17
10:27am April 10th 2017 via Hootsuite
Meiri: Normally, trophoblast invasion; pre-ecl fails. PP13 diverted in immune tolerance in preg mother; PP13 induces WBC apoptosis #MDxEU17
10:25am April 10th 2017 via Hootsuite
Meiri: DelT221 in PP13 among South Africans, enrichment for pre-eclampsia (7.2% vs. 2.4%) #MDxEU17
10:23am April 10th 2017 via Hootsuite
Meiri: Promoter polymorphism lowers expression; also stop codon for exon 2 (out of four total) of premature stop, truncating PP13 #MDxEU17
10:21am April 10th 2017 via Hootsuite
Meiri: (Background of ASPRE from EU funding organization https://t.co/OsM4a69ZSH ) #MDxEU17
10:20am April 10th 2017 via Hootsuite
Meiri: First trimester pts, Sanger seq of promoter region "-98 A/C" C base is protective allele. #MDxEU17
10:17am April 10th 2017 via Hootsuite
Meiri: Maternal blood PP13 - shows data from '08 ref on PP13 https://t.co/Q7G0d6lYlj in cases of pre-eclampsia during pregnancy #MDxEU17
10:16am April 10th 2017 via Hootsuite
Nice to meet @KaitlinSearfoss in-person here at #MDxEU17 to talk about live-tweeting conferences. Much harder than it appears...
10:14am April 10th 2017 via Hootsuite
Meiri: 150mg/d from 12-36wks. 10% of top 1:100 risk; went after one of several risk factors ID'd #MDxEU17
10:12am April 10th 2017 via Hootsuite
Meiri: 80% drop in pre-eclampsia at <34 weeks, manuscript submitted to NEJM. Aspirin enlarges spiral arteries - but why early? #MDxEU17
Meiri: Risk factors are age, weight, height, ethnicity, obstetric history. 14 centers thru-out EU. 100% prediction <32y, 44% >37y. #MD
10:09am April 10th 2017 via Hootsuite
Meiri: Signals sent to maternal blood. ASPRE project - first trimester prediction and preventioin via aspirin. #MDxEU17
10:08am April 10th 2017 via Hootsuite
Meiri: 2-5% of circul in normal life; blood volume inc 50X during development. Placental remodeling of spiral. Preecl. - no remodel #MDxEU17
10:07am April 10th 2017 via Hootsuite
Meiri: Fetus has many affects - obesity, diabetes in adolescence, physiol injury. Pts born under pre-eclampsia likely to be affected#MDxEU17
10:06am April 10th 2017 via Hootsuite
Meiri: Pre-eclampsia many underlying causes. 59K mothers die of this every year; CNS seizures, hepatic failure, platelet disorder #MDxEU17
10:05am April 10th 2017 via Hootsuite
Hamutal Meiri (ASPRE Is) Polymorphism in the promoter region of the placenta protein 13/LGALS13 gene as biomarker for pre-eclampsia #MDxEu17
10:03am April 10th 2017 via Hootsuite
RT @JosephVacca: Welcome #mdxeu17 attendees! Please make note of the dedicated exhibit hall/poster viewing hours. https://t.co/qKDdYTDnNj
9:50am April 10th 2017 via Twitter Web Client
RT @kaitlinsearfoss: Hear from Ida Vogel on Chromosomal Microarray in #Prenatal #Diagnostics– Lessons from More Than 1500 Analyses. #MDxEU17
9:49am April 10th 2017 via Hootsuite
Q: What limitations of NGS need to be overcome? Nevado: Prenatal NGS may not be a crazy idea, aCGH for prenatal impt #MDxE17
9:12am April 10th 2017 via Hootsuite
Nevado: Mentions in conclusion - is sequencing from the start (birth) crazy (referring to '13 Nature news piece)? No it isn't. #MDxE17
9:11am April 10th 2017 via Hootsuite
Nevado: Future - want to replace conven. karyotyping with invasive prenatal samples w/aCGH. NIPT is not a Dx test; it is screning #MDxE17
9:09am April 10th 2017 via Hootsuite
