Diehn: Adv NSCLC, looking at L858R, Exon 19 del, T790M: Sen 93%, Spec 100%. #AACR17
11:14am April 4th 2017 via Hootsuite
Diehn: Built models to suppress systematic error further - 'background polishing'. integrated digital error suppression (IDES) #AACR17
11:13am April 4th 2017 via Hootsuite
Diehn: Increasing LOD by increasing muts - cp 1 vs 8. Shows all base changes from 0 to 0.03% - systematic G>T, C>T, G>A, T>C #AA
11:12am April 4th 2017 via Hootsuite
Diehn: wanted to increase recovery, decrease error, more mutations '16 Nature Biotech https://t.co/ieiO0VyxvE #AACR17
11:11am April 4th 2017 via Hootsuite
Diehn: That's 2.5 per million. '14 ref https://t.co/MQDWcwRCQE Showed Stg IIB NSCLC pre-Tx and 5mos later from paper #AACR17
11:10am April 4th 2017 via Hootsuite
Diehn: Describes CAPP-Seq, 300genes, hyb capture-based, sens 0.002% generalized; 0.0025% personalized #AACR17
11:07am April 4th 2017 via Hootsuite
Diehn: After recurrence, systemic Tx response, resistance var already being done. #AACR17
11:06am April 4th 2017 via Hootsuite
Diehn: Non-invasive genotyping routinely done for a number of tumor types. Localized disease next, MRD, surveillance (repl imaging) #AACR17
Max Diehn (Stanford CA) Personalized cancer detection and monitoring via deep sequencing of circulating tumor DNA #AACR17
11:03am April 4th 2017 via Hootsuite
Q: Real-time pharmacodynamic modulation? Haber: Most 4w post-Tx. Still experimental stage. #AACR17
Q: Applied to urine CTCs? Haber: Haven't done, iChip is for 1 in a billion; urine is acellular. Other microfluidics also may apply #AACR17
11:02am April 4th 2017 via Hootsuite
Q: Culture artifact vs original CTC? Haber: 2-4 mo to culture, do repeat sampling. Same pt, same mut's. Need to validate back to pt #AACR17
11:01am April 4th 2017 via Hootsuite
Haber: Impt of high-prevalence disease: PPV: 10% prev -> 92% TPs. 0.1% prev -> 9% TPs. 0.01% -> 0.9% TPs #AACR17
10:58am April 4th 2017 via Hootsuite
Haber: And also distinguish between aging-associated molecular abnormalities. And high-risk population very impt. #AACR17
10:57am April 4th 2017 via Hootsuite
Haber: droplet PCR, sequence-based will help sens/spec. Need for tissue-of-origin, distinction from indolent proliferation #AACR17
10:56am April 4th 2017 via Hootsuite
Haber: Idea - serial monitoring for higher liver CTC-score, AFP, +/- liver ultrasound. As a screen need very high sens/spec assays #AACR17
Haber: Shows AUC of 0.8 for ROC of untreated HCC. For early detection: AFP and CTC-score together can give orthogonal higher det rt #AACR17
10:55am April 4th 2017 via Hootsuite
Haber: Liver cancer - HCC 80-90% in cirrhosis, 0.5% to 8%/yr. 200M at high risk from chronic Hep B. https://t.co/ABsLUDnT7U #AACR17
10:53am April 4th 2017 via Hootsuite
Haber: Showing markers that show up during progression of CTC-derived markers in prostate cancer. #AACR17
10:51am April 4th 2017 via Hootsuite
Haber: Now showing 'do not post' data - #AACR17 poster abstract from yesterday: https://t.co/33ic7dAW6y
10:48am April 4th 2017 via Hootsuite
Haber: '13 Science https://t.co/4Wjsgp9lzh ref on prostate cancer CTC work. After single-cell iChip enrichment, char, then sc cDNA #AACR17
10:46am April 4th 2017 via Hootsuite
Haber: Selecting single CTCs via color, then char by RNA-Seq. Now doing ddPCR from single cells. For prostate CTCs, 8-20 markers #AACR17
10:44am April 4th 2017 via Hootsuite
Haber: Acquired HER2 heterogeneity. Janssen and MGH commercializing technology. Next work: toward RNA-based diagnostics. #AACR17
10:42am April 4th 2017 via Hootsuite
Haber: Shows drug sens of cultured CTCs. Then dynamic equilibrium between cell states. HER2+ acq '16 ref https://t.co/RR0cxvE0Vi #AACR17
10:40am April 4th 2017 via Hootsuite
Haber: Can be cultured ex-vivo, 'as few as 200 cells' '14 ref https://t.co/7anICCqYES and shows new muts appearing during trtmt #AACR17
10:39am April 4th 2017 via Hootsuite
Haber: Shows instrument, cells from FNA, and staining of melanoma CTCs. Can monitor longitudinally '13 ref https://t.co/tPwx4hPeP3 #AACR17
10:37am April 4th 2017 via Hootsuite
Haber: Then inertial focusing, then magnetophoresis. Now a CTC-iChip is fixed - 3 different regions, 10mL blood/h up to 30mL #AACR17
10:36am April 4th 2017 via Hootsuite
Haber: Working with Toner of MGH, the iChip '13 ref https://t.co/izCxWhi8OH Anti-CD45 mag beads; hydrodynamic sorting... #AACR17
10:35am April 4th 2017 via Hootsuite
Haber: Intravastation from primary Noninvasive monitoring, and potential for early detection #AACR17
10:34am April 4th 2017 via Hootsuite
Daniel Haber (Mass Gen MA) RNA-based analysis of circulating tumor cells #AACR17
10:32am April 4th 2017 via Hootsuite
Nelson: 'And some of you will figure this out.' #AACR17
10:04am April 4th 2017 via Hootsuite
Nelson: Of smokers 16% will get cancer; exposures 80%, inheritance 40%, replication as many as 65%. 185%? #AACR17
Nelson: Per cell division, about 5-10 bases are mutated per cell division. Shows figure from '15 ref https://t.co/kr7LxPDSBv #AACR17
10:03am April 4th 2017 via Hootsuite
Nelson: Shows collard greens: should be a superfood. 'The best way to cook greens is not in bacon grease' #AACR17
10:01am April 4th 2017 via Hootsuite
Nelson: #AACR17 Data from restaurant meat mutagens '00 ref https://t.co/8SeoY8zKLI
10:00am April 4th 2017 via Hootsuite
Nelson: Obesity rates - sobering, map of BMI >30 in the US. Overcooking, charbroiling creates carcinogens. Heterocyclic amines #AACR17
9:58am April 4th 2017 via Hootsuite
Nelson: 'Stop Smoking!' - no question it causes cancer. Diet: most what we know comes from questionnaires; a tricky question #AACR17
9:57am April 4th 2017 via Hootsuite
Nelson:Environment vs inheritance '06 WHO ref https://t.co/81usyoNyuj data of Japanese in Japan vs Japanese in the US and cancer #AACR17
9:56am April 4th 2017 via Hootsuite
Nelson: 44.7k Scandinavian twin pairs, Prostate ca 42%, CR 35%, Breast 27% evidence '00 ref https://t.co/lkWOsFYKCS #AACR17
9:55am April 4th 2017 via Hootsuite
Nelson: What causes cancer in people? breathing, eating, drinking, parents, bad luck,.. hints from genetics of twins #AACR17
9:53am April 4th 2017 via Hootsuite
Nelson: Fast forward to HGP. 'Each one of you are 99.9% identical'. >3M differences, cancers have at least 10K mutations each #AACR17
9:51am April 4th 2017 via Hootsuite
Nelson: Normal cells have genes that suppress cancers '69 Nature https://t.co/AmqHDCoL6X #AACR17
Nelson: Shows gag, env, pol genes: the cancer gene derived from a normal host gene. Src gene: the idea of defective oncogenes #AACR17
9:49am April 4th 2017 via Hootsuite
Nelson: RSV cancer gene char by Varmus and Bishop, 'an inelegant time for dress' commenting on their attire in the photo #AACR17
9:48am April 4th 2017 via Hootsuite
Nelson: #AACR17 Shows chromosome stain from 1966 ref https://t.co/wUBGux7yiW Viruses - shows image from Rous 1911 https://t.co/VTsLtDm7Wl
9:47am April 4th 2017 via Hootsuite
Nelson: Early 1900's: Boveri speculated chromosomes carry genetic material, cancers viewed with abnormal chromosomes. #AACR17
9:44am April 4th 2017 via Hootsuite
Nelson: Virchow in the 1800's: activation of dormant cells; caused by irritation (inflammation) in the tissues. 'Chronic irritation' #AACR17
Nelson: Hippocrates - carcinos; Celsus translated carcinos to cancer; Galen applied oncos for tumors, from Greek for mass. #AACR17
9:43am April 4th 2017 via Hootsuite
Nelson: Prostate cancer back to Egypt - 2nd oldest cancer case 2,250 years ago. Edwin Smith Papyrus: Imhotep describing breast ca #AACR17
9:41am April 4th 2017 via Hootsuite
Nelson: Outline - what is cancer (hallmarks), and what causes cancer (genetics) #AACR17
9:40am April 4th 2017 via Hootsuite
