Jarosz: If two adjacent smpls share one of the two indexes, get index misassignment. Index hopping when samples mulitplexed w/cap #NGDx17
10:05am August 16th 2017 via Hootsuite
Jarosz: Introduces NGS workflow, and goes onto sample crosstalk problems, and dual indexing strategy available from Illumina w/20 #NGDx17
10:04am August 16th 2017 via Hootsuite
Mirna Jarosz (IDT) Nucleic acid technologies to enable clinical diagnostics - sample integrity to multiplex PCR #NGDx17
10:03am August 16th 2017 via Hootsuite
Epstein: Induce PBMC population w/tumor cells co-culture, isolate MDSC w/anti-CD33. Dev a suppression assay w/flow cytometry readout #NGDx17
9:10am August 16th 2017 via Hootsuite
Epstein: Also GM-CSF PGE2 IL-1b. Tumor cells produce these factors to inhibit MDSC. Shows BALB/c mouse data #NGDx17
9:07am August 16th 2017 via Hootsuite
Epstein: Function of MDSC is to prevent autoimmunity and modulate end-responses. MDSC factors ID'd from tumor cells IL6 TGFb VEGF #NGDx17
9:06am August 16th 2017 via Hootsuite
Epstein: Myeloid-derived suppressor cells (MDSC) are induced by the tumor by a number of cytokines, and closely associated w/Treg #NGDx17
9:03am August 16th 2017 via Hootsuite
Epstein: In about 50% of cancers are HLA negative. Their hypothesis - diverse mechanisms (immune evasion, direct supp, indirect) #NGDx17
9:00am August 16th 2017 via Hootsuite
Epstein: Cancer a balance between active tumor regression, and suppressed response that allows tumor growth. #NGDx17
8:57am August 16th 2017 via Hootsuite
Epstein: Recurrent and metastatic disease is the greatest mobidity, mortality. There are critical window for improving outcomes #NGDx17
8:56am August 16th 2017 via Hootsuite
Epstein: NeoAg can be given back, but you have to deal with suppression. Rare pop'ln of cells, <1% in normal. #NGDx17
8:55am August 16th 2017 via Hootsuite
Alan Epstein (Univ So Calif) The MDSC clinical assay for the immunodiagnosis and monitoring of solid tumors #NGDx17
8:54am August 16th 2017 via Hootsuite
Q: In silico validity? Mandal: Not very good right now (well, 'still') need to acknowledge and improve #NGDx17
8:53am August 16th 2017 via Hootsuite
Q: Min number of muts? Mandal: Not just the number, it's the quality. If 1 mut is immunogenic, that's all you need #NGDx17
8:52am August 16th 2017 via Hootsuite
Q: Company that does co-culturing? Mandal: Genocea More info on ID of specific, individual T-cell antigens https://t.co/zYZl29Hx1F #NGDx17
8:51am August 16th 2017 via Hootsuite
Mandal: #NGDx17 FInishes with a figure from a '16 review https://t.co/n6K7vxkFNZ on how the future of I-O may play out.
8:49am August 16th 2017 via Hootsuite
Mandal: Suggests a neoAg pipeline: PMBCs, take autologous dendtritic cells, co-culture to ID the antigen. #NGDx17
8:47am August 16th 2017 via Hootsuite
Mandal: WES and RNASeq give T-cell repertoire, eval clonality, look at neoAg, Class I/II processing. #NGDx17
8:45am August 16th 2017 via Hootsuite
Mandal: #NGDx17 '16 JCI Insight https://t.co/lKGMLmACvF Head and Neck - shows high NK cell infiltration; correlated to positive outcome
8:44am August 16th 2017 via Hootsuite
Mandal: Shows clonality in these tumors. Also defectsin Ag presentatio can cause immune escape, incl defects in methylation #NGDx17
8:43am August 16th 2017 via Hootsuite
Mandal: MMR deficiency across cancers '15 Nature Med https://t.co/vg3qFkijJI #NGDx17
8:41am August 16th 2017 via Hootsuite
Mandal: #NGDx17 '15 NEJM https://t.co/vd9w1Tb1Md shows MMR deficiency. MMR causes slippage, MSH2/6/MLH1/PMS2. Any one impairs repair
Mandal: Shows fig from Rizvi '15 of distribution of DNA repair genes in NSCLC responders to Pembro #NGDx17
8:39am August 16th 2017 via Hootsuite
Mandal: In NSCLC, shows mutational burden w/Pembro '15 Science https://t.co/lNYrslYgX7 #NGDx17
8:38am August 16th 2017 via Hootsuite
Mandal: Snyder NEJM '14 shows mut load correlates with clinical benefit in melanoma / ipi https://t.co/7rzvg7XnPH #NGDx17
8:37am August 16th 2017 via Hootsuite
Mandal: Shows neoAg by MHC to CD8+ cells. Fig from Chen and Mellman '13 Cancer-Immune cycle and promotion of anti-tumor activity #NGDx17
8:36am August 16th 2017 via Hootsuite
Mandal: Shows type of neo-Ag. Type I, II is invisible, mutation causes HLA presentation. III is frameshift casting novel peptide #NGDx17
8:35am August 16th 2017 via Hootsuite
Mandal: I-O is a fire extinguisher. Shows Ferris NEJM 2016 https://t.co/yWtpnHnWyi figure on poor prognosis of PD1/L1 as biomarker #NGDx17
8:34am August 16th 2017 via Hootsuite
Mandal: If cancer is a fire, there may be different types of fuel: p53, RAS, BRAF, PIK3, EGFR. Targeted Rx = other fuel sources #NGDx17
8:31am August 16th 2017 via Hootsuite
Raj Mandal (MSKCC NY) The genomic and immunologic determinants of response to head & neck cancer immunotherapy #NGDx17
8:30am August 16th 2017 via Hootsuite
New post: A door opens at Pillar Biosciences https://t.co/aeiuCtplgb
3:31am August 16th 2017 via WP to TWTR on Yuzuki.org
Sequencing Patient’s Circulating Tumor Cells Reveals Possible Treatment Approaches | GenomeWeb https://t.co/rhfd68xtEs
11:40pm August 15th 2017 via Hootsuite
Metastatic, Relapsed Breast Cancer Genomic Study Reveals Relationship to Primary Tumors | GenomeWeb https://t.co/uFdDiaHDwd
10:40pm August 15th 2017 via Hootsuite
A Competitor Rises to Watson’s Defense | Praful Krishna | LinkedIn https://t.co/YBD7XDeySJ
9:44pm August 15th 2017 via Hootsuite
ASCO Updates NSCLC Guidelines With New Directives for Genomic Testing | GenomeWeb https://t.co/CEASz7QcVL
9:35pm August 15th 2017 via Hootsuite
The Mediterranean diet works for the wealthy and well-educated — Quartz https://t.co/NgtCqFOQJg
4:05pm August 15th 2017 via Hootsuite
How Not to Die: ‘Stupid Cancer’ Community Helps Angry Young People Live | Newsweek https://t.co/3Q0bjhcRQL
Is the world really better than ever? | The Guardian https://t.co/Bj33PPdyRL
3:55pm August 15th 2017 via Hootsuite
RT @joe_pickrell Blog post: it is time to replace genotyping arrays with sequencing https://t.co/bHmOxh3W26 https://t.co/TQa3aaQrld
2:55pm August 15th 2017 via Hootsuite
Science should be taught like art or music: grab a test tube and have a go | Opinion | The Guardian https://t.co/yfUXbqAu27
2:35pm August 15th 2017 via Hootsuite
RT @ODILeeds: Cities drowning in #data turn to 'citizen scientists' - https://t.co/J2ZQI08HCt #datascience #opendata
1:55pm August 15th 2017 via Hootsuite
RT @GenomeAtlantic What can genetic testing really tell you? https://t.co/LeklP1Fube via @PopSci
1:50pm August 15th 2017 via Hootsuite
RT @guardianscience ‘Most spectacular thing I’ve ever seen in my life’: US readies for total eclipse https://t.co/SYRkfdVng7
12:50pm August 15th 2017 via Hootsuite
RT @BioMickWatson Not all heroes wear capes https://t.co/XpJ2HGPKRk
11:50am August 15th 2017 via Hootsuite
MT @TrevorWBrown: At DC #NGDx17 Check out Russell Garlick’s talk @ 12:10 on Tue on our new ccfDNA ref materials! https://t.co/YNI75VR8TS
10:45am August 15th 2017 via Hootsuite
NSF launches new SBIR website - nice look! | NSF SBIR https://t.co/q50rJ7MVRM
9:40am August 15th 2017 via Hootsuite
This fall, Inova Health will launch its Personalized Health Accelerator - Technical.ly DC https://t.co/cZy0Hfblwx
8:35am August 15th 2017 via Hootsuite
RT @SmithGenomicMed: Did you know? @hudsonalpha’s @lizworthey is presenting @CHI_Healthtech #NGDx17 tomorrow. Learn more about her work htt…
6:52am August 15th 2017 via Hootsuite
RT @DeciBio: The Next Generation Dx Summit kicks off tomorrow in DC. Say hello to the DeciBio team if you are attending. #NGDx17 https://t.…
6:51am August 15th 2017 via Hootsuite
Appeals Court Affirms Thermo Fisher Scientific Does Not Infringe Enzo Patent | GenomeWeb https://t.co/VEqenxElMK
7:31pm August 14th 2017 via Hootsuite
