RT @vsbuffalo: Manipulating things 100 different ways before you can do a single useful thing with it is ~90% of bioinformatics. https://t.…
6:33am August 24th 2017 via Hootsuite
RT @medtechMarion: These areas will be impacted by AI George Poste #pmls2017 https://t.co/qLF8HGdndW
6:05am August 24th 2017 via Hootsuite
RT @medtechMarion: Something will break George Poste said #pmls2017 https://t.co/0IWnYSC5Cy
6:00am August 24th 2017 via Hootsuite
RT @lexnederbragt: BGI's 180 sequencers are churning out around 5 terabases of data per day #ngs #datadeluge http://bit.ly/h49cBo
12:38pm August 23rd 2017 via Twitter Web Client
RT @Dr_ThomasZ: At the speed of darkness...watch as #SolarEclipse2017 shadow moves across our beautiful planet at <1 mile/second; as seen
11:32am August 23rd 2017 via Hootsuite
RT @foxerinr: Novartis CEO opens door to cancer patient demanding ‘fair’ CAR-T pricing https://t.co/GBtFFOmtyY
RT @mdeziel: "Either write something worth reading, or do something worth writing about" — Ben Franklin
6:25am August 23rd 2017 via Hootsuite
New Post: Quanterix SR-Plex benchtop single-molecule digital detection technology | Next Generation Technologist https://t.co/rU1AfQqLwL
12:30pm August 22nd 2017 via Hootsuite
China’s embrace of embryo selection (preimplantation genetic diagnosis) raises thorny questions : Nature News https://t.co/WoO9k3tVlx
12:05pm August 22nd 2017 via Hootsuite
VelaDx Receives CE-IVD for NGS-Based HIV Genotyping Assay | GenomeWeb https://t.co/ybI3g8bQyc
11:20am August 22nd 2017 via Hootsuite
A Speedier Way to Catalog Human Cells (All 37 Trillion of Them) - The New York Times https://t.co/MAdBemXX3I @JShendure in the news
11:05am August 22nd 2017 via Hootsuite
TJU-Led Team Wins $2.7M NCI Grant to Study Circulating Tumor Cells in Breast Cancer | GenomeWeb https://t.co/cMNMTR6HKw
10:05am August 22nd 2017 via Hootsuite
Heart Disease Risk Alleles Reported In 17th Century Korean Mummy | GenomeWeb https://t.co/ZPDxOzKHrG
9:01am August 22nd 2017 via Hootsuite
Rapid DNA Act Signed Into Law | GenomeWeb https://t.co/NIYiwKHQKK
FYI @Pillar_Bio has an updated website https://t.co/gtiYACbCdQ if you are looking for NGS enrichment that's faster, better and...
7:46am August 22nd 2017 via Hootsuite
New post: Quanterix SR-Plex benchtop single-molecule digital detection technology https://t.co/rU1AfQqLwL
4:46am August 22nd 2017 via WP to TWTR on Yuzuki.org
Trump quietly signs new FDA user fee agreement into law | Stat News https://t.co/75890fbJMD
9:45am August 21st 2017 via Hootsuite
What a way to get your SO to read the manuscript you've worked so hard to write. https://t.co/iWb9Hxx9F5
7:20am August 21st 2017 via Hootsuite
RT @investinskane: Lund/Malmö join Silicon Valley, Eindhoven, Cambridge, MA in list of "smartest places on Earth". #lifescience #innovatio…
6:47am August 21st 2017 via Hootsuite
For those interested in what I'm doing now with @Pillar_Bio check out our new and shiny website: https://t.co/jPXCkve5Fj
6:08am August 21st 2017 via Hootsuite
In Nashville TN for the #GallatinEclipse event tomorrow. Going to be a blast! The Facebook page here: https://t.co/QCTayYlHnK
4:46pm August 20th 2017 via Hootsuite
Can a 'liquid biopsy' detect cancer and save lives? | Stat News https://t.co/uoUMORyBIw
9:55pm August 19th 2017 via Hootsuite
RT @wef: This Japanese doctor lived to 105. Here's his advice for a long life https://t.co/5zJRJpyLBD #Japan #ageing https://t.co/QuAn44hm6R
9:11pm August 19th 2017 via Hootsuite
RT @murtazalab: enjoyed presenting our #cfDNA quality assessment #ddPCR assay at #NGDx17. preprint for this work: https://t.co/3djL5Tc16P
8:45am August 19th 2017 via Hootsuite
RT @bill_easterly: Headlines we should see but don't: "World is now experiencing greatest child mortality reduction ever." https://t.co/5Np…
7:39am August 19th 2017 via Hootsuite
Schlappi: Future - multiplex both spp and ABX, CDC urgent threats (gonorrea may grow more slowly) #NGDx17
3:35pm August 18th 2017 via Hootsuite
Schlappi: With >50 samples, AUC 0.956 able to go from urine cup in <30 min. Can do 15 min E coli quick ID. #NGDx17
3:33pm August 18th 2017 via Hootsuite
Schlappi: Now just take treated and control w/ABX, shows difference in amplification. Shows ratio of 1.6x #NGDx17
3:31pm August 18th 2017 via Hootsuite
Schlappi: '14 rev https://t.co/ZVefT54cMc Have a single-molecule amplification in <15' w/optimized LAMP #NGDx17
3:30pm August 18th 2017 via Hootsuite
Schlappi: Another adorable video - the readout in a black showbox, taking a photo with a phone. #NGDx17
3:28pm August 18th 2017 via Hootsuite
Schlappi: Adorable video - 'so simple a kid can use it'. Shows blood sample filling by capillary flow. Slip = 'and now its done' #NGDx17
3:27pm August 18th 2017 via Hootsuite
Schlappi: SlipChip is 1000's of compartments, 2 glass slides. Load in a long channel, slip in 90 deg orientation #NGDx17
3:26pm August 18th 2017 via Hootsuite
Schlappi: Shows add'l organisms don't affect signal. W/51 samples, AUC = 0.985. So used LAMP (isothermal). Got signal in 5' #NGDx17
3:24pm August 18th 2017 via Hootsuite
Schlappi: qPCR is about a ratio of 2; dPCR is a 1.2 ratio. Need to resolve 1.4x within 20 min. Clin samples may have commensals #NGDx17
3:22pm August 18th 2017 via Hootsuite
Schlappi: Prior work still needed culture, plus 2h for ddPCR. Shows cartoon of submitted manuscript: UTI urine, +/- ABX, use a 1.1x #NGDx17
3:21pm August 18th 2017 via Hootsuite
Schlappi: 15 min exposure of antibiotics (ABX), shows 15 and 30 min difference w/dPCR. '16 ref https://t.co/FuEm2ZhyAW #NGDx17
3:18pm August 18th 2017 via Hootsuite
Schlappi: For UTI's: 10M visits/yr. Limited number of pathogens - 70% are E.coli, and four classes of antibiotics. #NGDx17
3:16pm August 18th 2017 via Hootsuite
Schlappi: Digital PCR: limited by pipetting accuracy. qPCR 1.3x in conc is 0.4 cT. vs. ddPCR to get quant #NGDx17
3:15pm August 18th 2017 via Hootsuite
Schlappi: Look at r23S as a proxy for growth. Problem w/real-time qPCR: not quantitative enough #NGDx17
3:14pm August 18th 2017 via Hootsuite
Schlappi: Need culture for phenotype and fast. DNA replication as organism grows. Treated: no replication. Copy number after 20 min? #NGDx17
3:13pm August 18th 2017 via Hootsuite
Schlappi: There are about 1000 genes for beta-lactamases ID to-date. Need 30-60 minute Dx for antibiotic susceptibility #NGDx17
3:12pm August 18th 2017 via Hootsuite
Schlappi: Many methods to tackle this - rapid Dx with POC. Traditional culture too slow. 'Dx uncertaintly drives irrational use' #NGDx17
3:11pm August 18th 2017 via Hootsuite
Schlappi: Reviews threat of antibiotic resistance (AR), spread of resistance. AMR est to cause 10M deaths (cancer is 8.2M) #NGDx17
3:09pm August 18th 2017 via Hootsuite
Travis Schlappi (CalTech CA) Minue pathogen-specific phenotypic antibiotic susceptibility test using digital LAMP Quantification #NGDx17
3:08pm August 18th 2017 via Hootsuite
Mirmira: Is all DNA created equal? Looking at DNA methylation may come up with panels that reflect disease pathogenesis #NGDx17
3:01pm August 18th 2017 via Hootsuite
Mirmira: Concl: Children w/obesity, exhibit heightened beta-cell death. Could be with systemic inflammation. ddPCR 'critical tech' #NGDx17
3:00pm August 18th 2017 via Hootsuite
Mirmira: Hypothesis: less resistant beta-cells die off, and then you do not have anything left. Now in human: longitudinal #NGDx17
2:57pm August 18th 2017 via Hootsuite
Mirmira: High-fat diet (HFD), mouse model where beta-cells can be killed. Shows methylation marker (unpubl) #NGDx17
2:56pm August 18th 2017 via Hootsuite
Raghu Mirmira (Indiana Univ) Digital PCR as a platform for the next generation of diabetes biomarkers #NGDx17
2:54pm August 18th 2017 via Hootsuite
Manne: Lots of focus on validation of biomarkers. Show busy chart from 2005 - where CDx comes in at the end, assisting physicians #NGDx17
2:47pm August 18th 2017 via Hootsuite
