April 2018

Q: Upper limit of multiplexing? Abudayyeh: #ampeurope2018 Now single-plex; 4-plex underway; Ag-Ab interaction dep, likely 10plex

12:09pm April 30th 2018 via Twitter Web Client

Q: What about commercialization? Abudayyeh: #ampeurope2018 1y conversations about how best to do this, inf disease, Ag, Gates Found

12:07pm April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 RNAi doesn't work well in nucleus, but their system works well there to understand mechanism

12:06pm April 30th 2018 via Twitter Web Client

Q: Exploiting lincRNA at all? Abudayyeh: #ampeurope2018 Cas13d new protein for modulating splicing; may expand SHERLOCK.

12:05pm April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Lateral flow is exciting as it is field-deployable. Pushing technology with more applications, variants.

11:53am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Thinking that six-plex is feasible for fluorescence; up to 10-plex on lateral flow. Working on sepsis.

11:52am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Shows results in lateral flow strips. Also showed EGFR ex 19 del. Published in Gootenberg et al '18

11:52am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Onto liquid biopsy with lateral flow: Brigham and Women's collab. RPA 30', LwaCas13a reaction, look for EGFR L858R

11:51am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Single-base Zika mutation det w/lateral flow. N139S assoc'd with microencephaly in Zika, showed results

11:50am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 (Distracted thanks to misspelling of author's name Myhrvold et al in this week's Science)

11:49am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Two application to human samples: Zika, 40 South Am samples. https://t.co/ZY2FWzjb5G

11:49am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Boosting signal via cyclic activator '17 https://t.co/mlQftW4AXH Cas13 + Csm6 RNAses

11:43am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Shows [aM] sens after 1h. 'Likely working down to single molecule'

11:42am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 In lateral flow strip Strepavidin strip capture, if FAM is attached or separated - at the second Ab line readout

11:41am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Portability w/a visual readout? Current method needs a fluoresent plate-reader. Used FAM + Biotin (FAM for Ag det)

11:40am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Getting quantitative: dilution series, optimized primer conc w/real-time readout.

11:39am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Explains multiplexing method - ortholog base preference motifs. Shows 4plex data.

11:39am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Tried freeze-drying reagents on paper-based Dx; 95C 2' for thermal lysis, 37C 20' RPA. Also [aM] sens

11:34am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 SHERLOCK - tested against Zika, from blood or urine. Shown to 1.25 cp/uL Science '17 ref

11:33am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Describes SHERLOCK - spec high sen enzymatic reporter unlocking. 1 buffer 2 aM sens https://t.co/mWY6K3uPdL

11:32am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Collateral RNA: w/a fluor and quencher, a sensing mechanism https://t.co/tQnnYyvZ0m

11:31am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Rapid potential for Dx NEJM '17 https://t.co/naaW0XrAYf

11:30am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Phage RNA, then any other nearby RNA. Useful for 'detection of any nucleic acid'.

11:29am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 New effectors - described here https://t.co/2mlqFEfEGy C2c2 now called Cas13a '16 Science https://t.co/h9MopgmClt

11:28am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Classifications https://t.co/30hZRzKWpn New ones: Class I has many genes vs Class II (Cas9, only 1 gene)

11:24am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Cas9 is the first; many other types of CRISPR systems '14 rev https://t.co/TIg4gQeKKe Emerging CRISPR dx

11:22am April 30th 2018 via Twitter Web Client

Abudayyeh: #ampeurope2018 Other areas - viral vectors, optogenetics, and gene editing CRISPR in particular.

11:20am April 30th 2018 via Twitter Web Client

Omar Abudayyeh (Broad Inst MA) #ampeurope2018 Harnessing novel CRISPR systems for transcriptome engineering and human health

11:19am April 30th 2018 via Twitter Web Client

Deitz: #ampeurope2018 Shows GEq vs unique coverage by input amount; nice correlation.

10:18am April 30th 2018 via Twitter Web Client

Deitz: #ampeurope2018 TKI T780M is 40-55%; T790M w/ampl is another 10%. Copies/mL plasma.

10:15am April 30th 2018 via Twitter Web Client

Deitz: #ampeurope2018 66 ng needs many mLs of plasma. Dynamic monitoring. 15/16 pts mutations det '16 https://t.co/uLF64mmIn4

10:10am April 30th 2018 via Twitter Web Client

Deitz: #ampeurope2018 Chart of tumor derived DNA; if 0.1%, need 1K GEq, 6.6ng. 0.01%, need 10K GEq, 66ng. 0.001%, 100K GEq, 660ng

10:09am April 30th 2018 via Twitter Web Client

Deitz: #ampeurope2018 Nice chart of sens, method, info, cost TAT from https://t.co/V3IhiWuOTK

10:08am April 30th 2018 via Twitter Web Client

Deitz: #ampeurope2018 Shows usefulness of early det, profile, MRD, Rx resp, Rx resis, ID of lethal clones 'a molecular autopsy'

10:07am April 30th 2018 via Twitter Web Client

Steffan Deitz (DKFZ): #ampeurope2018 Application of ctDNA in the context of NSCLC

10:04am April 30th 2018 via Twitter Web Client

Palma: #ampeurope2018 Shows K-M fig from paper, survival the same even if mutant KRAS.

9:50am April 30th 2018 via Twitter Web Client

Palma: #ampeurope2018 Detect and track molecular changes: understanding the patient journey. Fig from '17 https://t.co/o6n2IjClCU

9:47am April 30th 2018 via Twitter Web Client