Alix-Panabieres: >60% of Br Ca pts had CTC's expr PL-L1. Q: were enough cells analyzed? Still too early for screening, early det #AACR18
2:16pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 They were the first in '15 to show PD-L1 expression on Br Ca CTCs. Ref https://t.co/xbodEFvB4C
2:13pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 Fig from '17 ref https://t.co/WPYAMyohJ1 Points out DEPArray system for single CTCs w/Silicon Biosystems platform
2:12pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 Biology - CTC resistant to 'Anoikis apoptosis'. EMT, invasion or intravasation. CTCs can be molecularly char.
2:11pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 EpCAM+/-, shows staining within the drops. Now validating in Pr Ca pts, called EpiDrop technique, Cp w/CellSearch
2:10pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 Can look at PSA molecules/sec excretion (for example from LNCaP cell line) Shows heterogeneity of excretion
2:09pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 Based on what cells can shed for viability. '17 ref https://t.co/2GHZA1t1po Can use microdroplet encapsulation
2:08pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 Reviews fig from '14 https://t.co/DqheLOsImP Impt to target functional CTCs - viable based on short-term culture
2:06pm April 17th 2018 via Hootsuite
Alix-Panabieres: #AACR18 Real-time liquid biopsy - '13 Clin Chem https://t.co/n3ZGDgwlC8 CTC enrichment based on biology or physical props
2:05pm April 17th 2018 via Hootsuite
Catherine Alix-Panabieres (Montpellier FR): Molecular and functional characterization of circulating tumor cells in… https://t.co/6xDlhClm7v
2:04pm April 17th 2018 via Hootsuite
Catherine Alix-Panabieres (Montpellier FR): introductory comments on #AACR18 liquid biopsy 'facts and challenges'. 2010 liquid biopsy
2:02pm April 17th 2018 via Hootsuite
EdX course worth a look #AACR18 https://t.co/AKGNnJUjvY
1:58pm April 17th 2018 via Hootsuite
RT @aftimosp: That was one passionate and inspiring talk by @subhamadhavan on data sharing and data sharing standards. Well done! #AACR18 h…
1:57pm April 17th 2018 via Hootsuite
An #AACR18 poster author interview "Application of ONCOREVEAL Lung & Colon Cancer Panel in CRC samples and effect o… https://t.co/oDQqNZ
1:56pm April 17th 2018 via Hootsuite
Different strokes for different folks at #AACR18 for sure... (And agree that the coffee in the hall is better than… https://t.co/AQ0TsKPr4n
1:45pm April 17th 2018 via Hootsuite
Paten: #AACR18 GA4GH has standard API's: https://t.co/pQ7gx64gUC Essential modules here: https://t.co/c05G35VQIF Iterative and progressive
12:27pm April 17th 2018 via Hootsuite
Paten: #AACR18 And w/workspaces, can lower barriers to entry. Spin up a tensorflow, to launch Jupyter notebook.
12:23pm April 17th 2018 via Hootsuite
Paten: #AACR18 A sandbox: when you perform analysis in cloud, you can decide what data is popular, what features impt. GitHub to roll-back
12:22pm April 17th 2018 via Hootsuite
Paten: #AACR18 Workspaces: we are working with sensitive data; there are rules, so need a security perimeter to operate in the cloud
12:21pm April 17th 2018 via Hootsuite
Paten: #AACR18 Also recipes; CWL clinical workflow language, WDL workflow description language.
12:20pm April 17th 2018 via Hootsuite
Paten: Lightweight docker virtualized containers - but parameterized with CWL/WDL, and sharing CWL/WDL workflows. Links to source #AACR18
12:19pm April 17th 2018 via Hootsuite
Paten: #AACR18 Not via github - b/c it is a lot of work to implement to reproduce the parameters etc. https://t.co/Q6nmDqmnD2 built to solve
12:18pm April 17th 2018 via Hootsuite
RT @CancerWallonia: Top Influencers of #AACR18
12:17pm April 17th 2018 via Hootsuite
Paten: Reviews main points on what it means by modular (decomposable), community driven, open (not proprietary), stds-based (GA4GH) #AACR18
12:14pm April 17th 2018 via Hootsuite
Paten: For the HCA, currently early data submissions, July and Oct milestones. Medium blog post: https://t.co/FmHn2NRyfL #AACR18
12:13pm April 17th 2018 via Hootsuite
Paten: Analysis can be reimported. Search, download, and compute on all data. Web browser UI, command line, multi-clouds #AACR18
12:11pm April 17th 2018 via Hootsuite
Paten: And then to validate the data: all virtualized. Standardized analysis pipelines, full transcript scRNA-Seq, 2' scRNA-Seq #AACR18
12:10pm April 17th 2018 via Hootsuite
Paten: The data generators: many submitters, worldwide, diversity of biology, both specialists and cores. Common UI, API, metadata #AACR18
12:07pm April 17th 2018 via Hootsuite
Paten: Broad, EMBL, Chan-Zuckerberg, and UCSC organizations involved. Cloud-based, Open, Modular, Int'l. #AACR18
12:06pm April 17th 2018 via Hootsuite
Paten: Starts with the Human Cell Atlas project https://t.co/INtbrkT7Cl Data has to be available, a data-collection platform #AACR18
12:05pm April 17th 2018 via Hootsuite
Benedict Paten (UCSC CA) An introduction to the data biosphere #AACR18
12:04pm April 17th 2018 via Hootsuite
Lovly: Wants to address disparaties in cancer care, concludes with https://t.co/rRAc0cVHl2 - 10K visits/week, includes ctDNA content #AACR18
8:57am April 17th 2018 via Hootsuite
Lovly: Cell prolif study; points out TKI's approval for molecular char, and in-vitro work shows effectiveness ('off-label') #AACR18
8:51am April 17th 2018 via Hootsuite
Lovly: #AACR18 Had complication w/low platelet cts, shows struct of EGFR-RAD51 Loss of C-term kinase '16 ref https://t.co/KYg8nEvhUB
8:49am April 17th 2018 via Hootsuite
Lovly: 2nd case: 21yo female, light smoker, StgIV NSCLC No EGFR mut in ex18-21, no ALK rearr. FMI found EGFR-RAD51 #AACR18
8:48am April 17th 2018 via Hootsuite
Lovly: Reminds us that this came from a pt case, onto the biology. Other kinase domain duplications (KDD) - FMI found 600 cases #AACR18
8:45am April 17th 2018 via Hootsuite
Lovly: (Not able to post, work in progress, working out mechanism of heterodimerization via site-directed mut, and kinase assays) #AACR18
8:43am April 17th 2018 via Hootsuite
Lovly: Explains figure of TKI domain, and EC domain of EGFR from '06 Cell https://t.co/rpjzYfyz4P Hypoth: 2 kinase domains dimerize #AACR18
8:33am April 17th 2018 via Hootsuite
Lovly: Idea: EGFR-KDD activated thru constitutive intra-molecular asymmetric dimerization #AACR18
8:31am April 17th 2018 via Hootsuite
Lovly: Found evidence of recurrence in TCGA, MSKCC and FMI datasets. Sens to EGFR TKI's in vitro, adv understanding of EGFR fn #AACR18
8:30am April 17th 2018 via Hootsuite
Lovly: #AACR18 Describes 33yo male never smoker, StgIV Lu cancer, and a novel EGFR mutation called KDD '15 ref https://t.co/djM01wK23F
8:29am April 17th 2018 via Hootsuite
@jdidion Hi John, it said 'Wood et al submitted' so you'll have to setup a PubMed search (with Victor Velculescu) in a few weeks.
8:27am April 17th 2018 via Hootsuite in reply to jdidion
Christine Lovly (Vanderbilt Univ TN) Precision Medicine for Lung Cancer #AACR18
8:26am April 17th 2018 via Hootsuite
Hayes: #AACR18 CTC biomarker in pts progressing on Fulvestrant '16 https://t.co/wFCV1400YN
6:42pm April 16th 2018 via Hootsuite
Hayes: Shows transfection data from '13 ref - different ESR muts have different phenotypes. #AACR18
6:41pm April 16th 2018 via Hootsuite
Hayes: CTC genotyping for ER resistance in mBC: ESR1 mutations first reported in the '90s '13 ref https://t.co/vkJSbSsF1v #AACR18
6:40pm April 16th 2018 via Hootsuite
Hayes: 'We need to improve beyond CTC enumeration" - phenotyping, genotyping. #AACR18
6:36pm April 16th 2018 via Hootsuite
Hayes: #AACR18 Results '14 JCO of SWOG S0500 https://t.co/EvGVrXKEFg Swtiching chemo didn't help OS, chemo-resistant disease
6:34pm April 16th 2018 via Hootsuite
Hayes: SWOG S0500: Met BrCa - first line chemoRx. CTC <5 arm A, CTC >5 onto chemo, and split after 3w of Rx. Shows OS by arm #AACR18
6:33pm April 16th 2018 via Hootsuite
Hayes: CTC enumeration and prognosis: CTCs vast majority of data is from CellSearch.'04 ref https://t.co/PWekg21C3X mBC #AACR18
6:32pm April 16th 2018 via Hootsuite
