Cottrell #AGBT19 n=59, 23 new Dx. Thinking of N of 1 approach - paired tumor/normal WES, RNA-Seq, can also do micr… https://t.co/O16K6aWsBg

7:35pm February 28th 2019 via Twitter Web Client

Cottrell #AGBT19 Comprehensive protocol - to ID targeted Rx, ID clinical trial, make Dx, establish prognosis, det c… https://t.co/18kIXntmCF

7:33pm February 28th 2019 via Twitter Web Client

Catherine Cottrell (Nationwide Children's Hosp) #AGBT19 Design and implementation of a comprehensive genomic profi… https://t.co/pY53h4WEvS

7:30pm February 28th 2019 via Twitter Web Client

Nelson #AGBT19 PTEN, splice variants in Pr ca of the AR receptor readily detected, whether that variant is consiste… https://t.co/joHdFEFEJt

5:21pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Points out that the slides are reusable, a useful thing. Also that these studies were done quickly.

5:20pm February 28th 2019 via Twitter Web Client

Q: Pt mgmt in earlier stage? Nelson #AGBT19 Localized has made progress, but problem is sampling (whether aggressiv… https://t.co/XH5beKeKPt

5:17pm February 28th 2019 via Twitter Web Client

Q: Can vast hetergeneity allow further subsets for Rx? Nelson #AGBT19 First cut will clarify whether it's the same… https://t.co/MD158cmd5S

5:15pm February 28th 2019 via Twitter Web Client

Nelson #AGBT19 Concl - CRPC molecular landscape indicates 'many subtypes with therapeutic implications'. DSP to ass… https://t.co/nAWWSMRGXn

5:13pm February 28th 2019 via Twitter Web Client

Nelson #AGBT19 Shows transition data from AR+/NE-, to different states of genes from different ROI's, illustrating… https://t.co/RlBNbIMwVO

5:08pm February 28th 2019 via Twitter Web Client

Nelson #AGBT19 Series of experiments on 5 FFPE sections: RNA, protein. Reprod between 108-plex and 1412-plex, r2=0.… https://t.co/2AihKnv9Tf

5:04pm February 28th 2019 via Twitter Web Client

Nelson #AGBT19 Prostate ca cell states - double neg look like squamous cells, some molecular classification by gene… https://t.co/wNsVM40YmL

5:01pm February 28th 2019 via Twitter Web Client

Nelson #AGBT19 New Rx - potent pressure on lineage of AR. Prior to 2012 - tumor progressoin on AR+; seeing much mor… https://t.co/TWmDPhVHZE

5:00pm February 28th 2019 via Twitter Web Client

Nelson #AGBT19 Their focus on prostate cancer. 26K deaths in US, due to mPC. Reduce mortality by early det or preve… https://t.co/zChutQQQQj

4:59pm February 28th 2019 via Twitter Web Client

Peter Nelson (Fred Hutch CC) #AGBT19 Mapping the microenvironment composition in metastatic prostate cancer by mul… https://t.co/0uGgksGZAu

4:57pm February 28th 2019 via Twitter Web Client

McPherson #AGBT19 Shows other genes down-reg 'made sense'. Cp melanocytes, epidermis, and immune-rich. Concl: known… https://t.co/makzLT3zzN

4:55pm February 28th 2019 via Twitter Web Client

McPherson #AGBT19 Shows volcano plots - PRAME gene up-regulated. Shows piece of tape - PRAME and LINC00518 by qPCR.… https://t.co/1Eo64Bz89X

4:54pm February 28th 2019 via Twitter Web Client

McPherson #AGBT19 Serial sections of same regions: R2 = 0.96. For technical replicates R2 = 0.98. Melanocytes, staining.

4:51pm February 28th 2019 via Twitter Web Client

McPherson #AGBT19 Four classes (from common nevi to melanoma), and look at 108 genes. Illustrated 200 um ROI's ID'd… https://t.co/YBGffalkkL

4:50pm February 28th 2019 via Twitter Web Client

McPherson #AGBT19 Tissues are small, lots of areas of interest, lots of LCM and trying to sequence. Shows lots of d… https://t.co/YqTs3jxqhk

4:49pm February 28th 2019 via Twitter Web Client

McPherson #AGBT19 Shows KM curve for melanoma, common nevi (moles) are mimics. Molecular tests - IHC. Thought: gene… https://t.co/OBvEWkHjIb

4:48pm February 28th 2019 via Twitter Web Client

John McPherson (UC Davis) #AGBT19 Spatially resolving RNA biomarkers using GeoMx Digital Spatial Profiler for earl… https://t.co/ZAscUNv6pf

4:47pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Hyb&Seq: full-blown, 4096 seq chem working, contract mfr done, much faster, three posters (908...)… https://t.co/bfhwynx

4:45pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Technical details on DSP is poster 514 Friday 440pm; mapping pathways poster 614 also Friday 440pm.… https://t.co/2RmLPGpgwn

4:44pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Molecular profilers and imagers 'live in different worlds' Volcano plots, tSNEs, etc are where the… https://t.co/hq9EkJujDY

4:42pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Poster 514 image: barcoded probes along length of mRNA, and shows 10 data points per xcript. Dropou… https://t.co/y9oc4o0nzR

4:40pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Shows data from Poster 614, the chemokine and cytokine regions are different. Active invasive margin cp to inactive one.

4:38pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Genentech actually wrote up a review '18 https://t.co/TMzHl7ocld before the instrument was launched.

4:36pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 How the UV light is focused: digital micromirrors. Shows CRC; visible wavelength PanCK antibody, gr… https://t.co/OAKkmI8NyF

4:34pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Microcapillary drinks the probes off the tissue in X-Y coordinate space and deposits into a 96-well… https://t.co/VMEIvjMsbD

4:31pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Affinity probes can be FISH for RNA with oligos instead of fluors. Or oligo-labeled antibodies. The… https://t.co/BJyLlESZZu

4:30pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 4y ago in Gordon Mill's office at MD Anderson - want 100K things off a tissue slice. Instead of ass… https://t.co/LRRqUKxwh7

4:28pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 Will update on Hyb & Seq launch 2021. GeoMx DSP focuses on very difficult FFPE samples. Discovery -… https://t.co/NEyMQt

4:27pm February 28th 2019 via Twitter Web Client

Beechem #AGBT19 3D biology - mRNA, proteins, miRNAs, talked about here at AGBT for a few years. Now: as a microscop… https://t.co/7D6y6Xa5TF

4:26pm February 28th 2019 via Twitter Web Client

Joe Beechem (Nanostring) #AGBT19 Emphasis is not on technology of the GeoMx Digital Spatial Profiler, but rather on… https://t.co/pYnj2kaLCH

4:25pm February 28th 2019 via Twitter Web Client

Neafsey #AGBT19 Issue driven by the price of gold (!) and 'everyone is complicit'. Shows a gold toilet, and use as… https://t.co/9LcMSFa4OS

4:03pm February 28th 2019 via Hootsuite Inc.

Neafsey #AGBT19 Talks about Arteminsin resistance, worldwide distribution, evidence it is on the margin, and that r… https://t.co/MyprOYmYXW

4:00pm February 28th 2019 via Hootsuite Inc.

Daniel Neafsey (Harvard) #AGBT19 Rewinding the tape: Historical pathogen population genomics using selective genome… https://t.co/BmeJuZCIDk

3:56pm February 28th 2019 via Hootsuite Inc.

10X Genomics #AGBT19 Introduces Chromium Connect, cells to sequencing library automation in their suite. "I need my… https://t.co/7ulAWsOaLh

12:59pm February 28th 2019 via Twitter Web Client

10X Genomics #AGBT19 Visium Spatial platform available 'H2 2019'.

12:58pm February 28th 2019 via Twitter Web Client

10X Genomics #AGBT19 Improved resolution: 100um spots, 200um center-to-center. Next: denser features and resolution… https://t.co/GTxQW10LTk

12:57pm February 28th 2019 via Twitter Web Client

10X Genomics #AGBT19 Have already shortened (in 3 mos) from 3days down to 9h. Have increased sensitivity >2x; 3x tr… https://t.co/tARBPMc

12:55pm February 28th 2019 via Twitter Web Client

10X Genomics #AGBT19 Spatial is 'coming soon' - end-to-end. Protocols for every step, slide and reagent kits, softw… https://t.co/HghRWvmgNw

12:54pm February 28th 2019 via Twitter Web Client

10X Genomics #ABRF19 Human heart as part of human cell atlas; week 6.5 and 15 cell types, shows t-SNE plot, and det… https://t.co/YjxMgHnw6w

12:51pm February 28th 2019 via Twitter Web Client

10X Genomics #ABRF19 Paper also noted discordances. Biorxiv preprint - looking across multiple slices, and across tumors.

12:49pm February 28th 2019 via Twitter Web Client

10X Genomics #AGBT19 Can start with an image, or start with clustering of genes. In '18 Nature Comm… https://t.co/66424C3Jtd

12:46pm February 28th 2019 via Twitter Web Client

10X Genomics #AGBT19 Shows Pearson 0.97 biological replicates on different arrays. Power - two complementary dataty… https://t.co/PgYGhm5KJL

12:44pm February 28th 2019 via Twitter Web Client

10X Genomics #AGBT19 Announces spatial transcriptomics. reviews '16 Science https://t.co/wVxn4MeNR4 Demonstrated on… https://t.co/RRmlLkK8Gw

12:43pm February 28th 2019 via Twitter Web Client

Stubbington #AGBT19 VDJ database online https://t.co/TQYAd6x8Ds 2,336 TCR-Ag spec 10 Ags from first Nature paper; 2… https://t.co/PxkJU11wjI

12:39pm February 28th 2019 via Twitter Web Client

Stubbington #AGBT19 Dataset: 80M input CD8+ T-cells (from four donors). 44 pMHC multimers, 6 neg controls. Data from 200K single cells.

12:36pm February 28th 2019 via Twitter Web Client