#AACR19 Q:Antigen retrieval? Akoya Kennedy-Darling: Analysis and retrieval on the coverslip isn't standard, current… https://t.co/Qm7d3d0Xqm
2:33pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Aqua analysis invented '99. Patented '07, owned by Novartis in '13 (Navigate biopharma, limited dist.)
2:31pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: QIF: how deep the stain goes? Est only 1-1.5um. Depth may not be important, rather diffusion.
#AACR19 Akoya Q: How thick can CODEX go? Rimm: Up to 10um. Higher - can the Ab get to the tissue. Need to also optimize fluidics protocol.
2:30pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Q: One objection is archiving Rimm: Pretty soon we won't have glass at all for archiving (only digital).
2:29pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Q: Calibration - do you normalize across specimens? Rimm: Can't normalize out pre-analytical var's.
2:28pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Hypothesis - talking on Monday, MOA is on macrophages binding PD-L1; when inhibited, macrophage… https://t.co/axCwVIXxMQ
2:26pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Points to this https://t.co/LQ1dUZE83d and https://t.co/6bg3cC9kRu showing high correlation bet… https://t.co/2ZCbaqLsen
2:23pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Concl "Counting and measuring are related but not equal'. Counting more accurate with rare events.
2:21pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: PFS and OS of RECIST categories - retrospective analysis looking at the charts, validating their criteria.
2:17pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: The problem w/% cells pos: you still need a threshold. Points to this recent CCR piece… https://t.co/qm88PQI5tD
2:16pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Measuring cells with chromagens: percent positive cells, and count via phenotypes. QIF: what's… https://t.co/2fcud6T1Jk
2:14pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: "15y ago when we started everyone thought we were nuts, now everyone is doing (QIF)"
2:13pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Inter-site PD-L1 R2 = 0.81. Taking human judgement out - MITRE w/ QIF represents the first pros… https://t.co/a4aEOZgQvu
#AACR19 Akoya Rimm: Tonsil CD20 slides: new LEDs reduces unmixing issues. Inter- and intra-site. MITRE initial resu… https://t.co/vewOqKOb1y
2:12pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Measuring stain intensity; started with lymph, and cell line TMA ('control pellets') with neg,… https://t.co/ZPZpqeuxo7
2:10pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Standards for quantitative fluorescence - a standardization TMA. Their mIF panel: PD-L1, Cd8, F… https://t.co/elJHCecAdo
#AACR19 Akoya Rimm: A 6-plex panel: PDL1, PD1, CD8, CD68, Foxp3, tumor (CK/lung, Sox10%S100/mel).
2:08pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Results under embargo and will be published 'soon'. MITRE: have a drug, "a validation of the QI… https://t.co/oo9Lkw22hC
2:07pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: Interesting - combinations of modes - get to 0.802 (multiplex IF + IHC). QIF of 0.8 has a low n… https://t.co/L0bjNlLGbK
2:06pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: AUC of metaanalysis for measurements for I/O https://t.co/sTB22EpnNj
2:05pm March 31st 2019 via Twitter for iPhone
#AACR19 Akoya Rimm: At SITC: Steve Lu (Hopkins) to compare biomarker assay modalities (PD-L1) and make AUC curves.… https://t.co/kLamwfJ1Wc
2:03pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Rimm: His disclosure slide is busy because biotech is moving this from the lab to the clinic. Will ta… https://t.co/xQl864FR6j
2:02pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya David Rimm (Yale) Dir of Yale Pathology Tissue Services title: Immunofluorescence - from the lab to the clinic?
2:01pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Working on signal amplification (lowering damage to lower exposure times). Typically… https://t.co/xqkljH1Xta
1:59pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows cell proximity by cell-type from the 2D analysis of breast cancer data.
1:57pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: FF lymph node from breast cancer pt: 29 marker staining; DAPI stain with .fcs file (… https://t.co/a10Bg2Nh4T
1:55pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows overlay data of FFPE human renal cell carcinoma with CODEX tissue analysis, al… https://t.co/o4qcJ95VVK
1:53pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows signal saturation / noise with titer data for spec Abs. Look at +/- countersta… https://t.co/MEDIJ8XdRH
1:50pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Also comparing tissues to databases with known targets. Threshold image of signal/no… https://t.co/wNWNyOYu9S
1:49pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Microscopes are controlled directly by their software. Image processing SW and image… https://t.co/7lVmhA4eJ3
1:48pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Tissue slices onto coverslips, inserted onto stage + gaskets; fluids controlled by Akoya instrument.
1:47pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Dist of Abs 'across 4 channels'. Four fluors, can use Alexa 750. CODEX integrates wi… https://t.co/KWkdzdYkvu
1:46pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows no signal dropoff from cycle 1 to cycle 11. Reagents: kits for staining, conju… https://t.co/KEiI5x87c3
1:45pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows CD38 staining of signal ROI (threshold) cp to noise ROI. Shows high signal/noise, with low carryover.
1:43pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Repeating process 16+ times. Shows movie - human tonsil, shows same tissue stained 1… https://t.co/0o4siUXSWd
1:41pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: Shows instrument - CO-Detection by inDEXing - reagents, fluidics control, software.… https://t.co/oRBitRaOpg
#AACR19 Akoya Kennedy-Darling: Busy slide with TME - DC, NK, B, Th, Tk, adipocytes. Overlaying checkpoints - 39 markers.
1:40pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Kennedy-Darling: highly multiplexed platform CODEX - starts with description of TME: contributions fr… https://t.co/HSVrKAG4xc
1:39pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya Lo: Unmixed, annotated ROI's up to 9-colors using tunable filter tech. CODEX: Julia Kennedy-Darling Dir R&D
1:38pm March 31st 2019 via Twitter Web Client
#AACR19 Akoya CEO David Lo: Phenoptics 2.0 is a Perkin Elmer-dev system, 7 color Opal for Polaris, 6 min (10x-20x faster) whole slide scans.
1:37pm March 31st 2019 via Twitter Web Client
Check out @AkoyaBio at the Exibitor Spotlight B now at #AACR19 Brian McKelligon CEO https://t.co/oyATg6uM1x
1:31pm March 31st 2019 via Twitter for iPhone
Hey #AACR19 get your #HappyCellfie @miltenyibiotec booth 2936 and get a photo and cool ‘Carl Carcinoma’ plushie https://t.co/caJj2VYdPK
1:10pm March 31st 2019 via Hootsuite Inc.
RT @sciam: A new way to fight cancer: metabolic therapy is showing promise in robbing malignant cells of their prim… https://t.co/66CrzaN0Zx
8:10pm March 30th 2019 via Hootsuite Inc.
RT @AACR: Unable to attend #AACR19? You will be able to watch the livestream of Sunday’s Opening Ceremony and Openi… https://t.co/CoMXUfSiiz
7:05pm March 30th 2019 via Hootsuite Inc.
RT @MarceloCorassa: Dr. Papadopoulos on the potential of ctDNA on early stage cancers. Can it be a potential biomarker to guide adjuvant th…
4:54pm March 30th 2019 via Hootsuite Inc.
RT @ChristianRolfo: Nice summary of ctDNA across tumors S. Dawson from Melbourne at #AACR19 @isliquidbiopsy https://t.co/SweQaylzWK
4:47pm March 30th 2019 via Hootsuite Inc.
RT @LabRoots: Hear from Kiana Aran, Ph.D. on her recent Nature publication on the CRISPR-Chip, a gene detecting biosensor, at Molecular Dia…
1:23pm March 30th 2019 via Buffer
SeqOnce Biosciences Launches RhinoSeq Rapid DNA Library Preparation Kit - SeqOnce https://t.co/cNASvgrwyd
1:22pm March 30th 2019 via Buffer
New drugs that unleash the immune system on cancers may backfire, fueling tumor growth | Science/AAAS https://t.co/HAbHh7TmG2
12:21pm March 30th 2019 via Buffer
