Here is a handy list of ‘fundamentals of next-generation sequencing’ posts I wrote way back in 2012, that are as useful today as it was when it was written, for those just starting out.
And for good measure I’ve included third-generation methods (single-molecule sequencing) and a few posts about the technologies in that area.
Lastly targeted selection, and the importance of phasing, which will become increasingly important. (Note to self – write up 10x Genomics once I speak with a real-world user.)
Next-Generation Sequencing – its historical context
Next Generation Sequencing – A Few Fundamental Concepts
Next Generation Sequencing – Library Preparation
Next Generation Sequencing – Template Preparation
Next Generation Sequencing – Sequencing by Pyrophosphate Release
Next Generation Sequencing – Sequencing by Reversible Terminators
What’s so special about single molecule sequencing?
Oxford Nanopore, the first nanopore-based sequencing technology
Single Molecule Sequencing – Pacific Biosciences and their method
An Overview of NGS Targeted Selection Methods and Marketplace
Complete Genomics’ Long Fragment Read Technology and Haplotype Phasing
View Comments