ampliseq – Next Generation Technologist

Ion AmpliSeq™ Exome – 294,000 primer pair PCR

July 1, 2013 by Dale Yuzuki

Ion Torrent Exome, courtesy of Life Technologies

Today Ion Torrent launches a neat product based upon a highly multiplexed technology called AmpliSeq, called the Ion AmpliSeq Exome, covering >97% of the CCDS regions. But before getting into the details, a little context is in order.

An Overview of NGS Targeted Selection Methods and Marketplace

July 11, 2012 by Dale Yuzuki

targeted selection methods and marketplace illustration — Photo courtesy of user {a href=”http://www.flickr.com/photos/vizzzual-dot-com/”}viZZZual.com{/a} via Flickr.

Although the whole genome versus whole exome discussion was held previously, details around the methods of selecting out the whole exome have been not discussed (also called ‘targeted selection’), and the wide array of methods, costs, and effort required can be a rather complicated affair.